@article {4501,
	title = {Lack of association between endothelial nitric oxide synthase gene polymorphisms and risk of premature coronary artery disease in the Greek population},
	journal = {Acta Cardiologica},
	volume = {63},
	year = {2008},
	note = {Cited By :16Export Date: 16 October 2015},
	month = {2008},
	pages = {609 - 614},
	abstract = {Objective - Genetic polymorphisms in the gene for endothelial nitric oxide synthase have been considered as potential risk factors for the development of coronary artery disease in some populations. Methods - We studied two polymorphisms of the NOS3 gene, the VNTR in intron 4 (4VNTR) and the Glu298Asp polymorphism in exon 7, in relation to the existence of premature coronary artery disease and the occurrence of myocardial infarction. A total number of 370 individuals of the Greek population was examined by PCR-RFLP method. The patient group consisted of 209 subjects, aged less than 58 years presenting symptomatic coronary artery disease, documented by coronary angiography. Results - The frequencies for bb, ab and aa genotypes of 4VNTR polymorphism were 0.67, 0.29, 0.04, respectively, for the patient group and 0.73, 0.24, 0.03 for the control group. The frequencies for GG (Glu/ Glu), GT (Glu/Asp), TT (Asp/Asp) of the Glu298Asp polymorphism were 0.52, 0.41, 0.07, respectively, in patients compared to 0.47, 0.46, 0.07, in control subjects. Statistical analysis indicated that there are no significant differences in the frequencies of the genotypes between patients and control subjects for both polymorphisms. The combined analysis of the two polymorphisms indicated no synergistic effect of the a and T alleles on coronary artery disease. Conclusions - We have found no evidence for association between the a allele of the 4VNTR polymorphism, or the T allele of Glu298Asp polymorphism and the risk for premature coronary artery disease or occurrence of myocardial infarction. Furthermore, no synergistic contribution of these polymorphisms to the development of premature coronary artery disease has been observed.},
	keywords = {Adult, Age Factors, Aged, Alleles, angiocardiography, article, Asparagine, aspartic acid, cardiovascular risk, Case-Control Studies, controlled study, Coronary artery disease, Endothelial nitric oxide, endothelial nitric oxide synthase, Endothelium, Vascular, exon, Exons, Female, genetic association, genetic polymorphism, Genotype, glutamic acid, Glutamine, Greece, heart infarction, human, Humans, Introns, major clinical study, Male, Middle Aged, Minisatellite Repeats, Myocardial Infarction, Nitric oxide, Nitric Oxide Synthase, Nitric Oxide Synthase Type III, NOS3 gene polymorphism, polymerase chain reaction, Polymorphism, Genetic, Polymorphism, Restriction Fragment Length, restriction fragment length polymorphism, Risk factor, Risk Factors, variable number of tandem repeat},
	url = {http://www.scopus.com/inward/record.url?eid=2-s2.0-56049104685\&partnerID=40\&md5=a456112407dbb24c44b3689bcb78dbbb},
	author = {Vasilakou, M. and Votteas, V. and Kasparian, C. and Pantazopoulos, N. and Dedoussis, G and Deltas, C. and Nastos, P. and Nikolakis, D. and Lamnissou, K.}
}
