<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Vasilakou, M.</style></author><author><style face="normal" font="default" size="100%">Votteas, V.</style></author><author><style face="normal" font="default" size="100%">Kasparian, C.</style></author><author><style face="normal" font="default" size="100%">Pantazopoulos, N.</style></author><author><style face="normal" font="default" size="100%">Dedoussis, G</style></author><author><style face="normal" font="default" size="100%">Deltas, C.</style></author><author><style face="normal" font="default" size="100%">Nastos, P.</style></author><author><style face="normal" font="default" size="100%">Nikolakis, D.</style></author><author><style face="normal" font="default" size="100%">Lamnissou, K.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Lack of association between endothelial nitric oxide synthase gene polymorphisms and risk of premature coronary artery disease in the Greek population</style></title><secondary-title><style face="normal" font="default" size="100%">Acta Cardiologica</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Adult</style></keyword><keyword><style  face="normal" font="default" size="100%">Age Factors</style></keyword><keyword><style  face="normal" font="default" size="100%">Aged</style></keyword><keyword><style  face="normal" font="default" size="100%">Alleles</style></keyword><keyword><style  face="normal" font="default" size="100%">angiocardiography</style></keyword><keyword><style  face="normal" font="default" size="100%">article</style></keyword><keyword><style  face="normal" font="default" size="100%">Asparagine</style></keyword><keyword><style  face="normal" font="default" size="100%">aspartic acid</style></keyword><keyword><style  face="normal" font="default" size="100%">cardiovascular risk</style></keyword><keyword><style  face="normal" font="default" size="100%">Case-Control Studies</style></keyword><keyword><style  face="normal" font="default" size="100%">controlled study</style></keyword><keyword><style  face="normal" font="default" size="100%">Coronary artery disease</style></keyword><keyword><style  face="normal" font="default" size="100%">Endothelial nitric oxide</style></keyword><keyword><style  face="normal" font="default" size="100%">endothelial nitric oxide synthase</style></keyword><keyword><style  face="normal" font="default" size="100%">Endothelium, Vascular</style></keyword><keyword><style  face="normal" font="default" size="100%">exon</style></keyword><keyword><style  face="normal" font="default" size="100%">Exons</style></keyword><keyword><style  face="normal" font="default" size="100%">Female</style></keyword><keyword><style  face="normal" font="default" size="100%">genetic association</style></keyword><keyword><style  face="normal" font="default" size="100%">genetic polymorphism</style></keyword><keyword><style  face="normal" font="default" size="100%">Genotype</style></keyword><keyword><style  face="normal" font="default" size="100%">glutamic acid</style></keyword><keyword><style  face="normal" font="default" size="100%">Glutamine</style></keyword><keyword><style  face="normal" font="default" size="100%">Greece</style></keyword><keyword><style  face="normal" font="default" size="100%">heart infarction</style></keyword><keyword><style  face="normal" font="default" size="100%">human</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Introns</style></keyword><keyword><style  face="normal" font="default" size="100%">major clinical study</style></keyword><keyword><style  face="normal" font="default" size="100%">Male</style></keyword><keyword><style  face="normal" font="default" size="100%">Middle Aged</style></keyword><keyword><style  face="normal" font="default" size="100%">Minisatellite Repeats</style></keyword><keyword><style  face="normal" font="default" size="100%">Myocardial Infarction</style></keyword><keyword><style  face="normal" font="default" size="100%">Nitric oxide</style></keyword><keyword><style  face="normal" font="default" size="100%">Nitric Oxide Synthase</style></keyword><keyword><style  face="normal" font="default" size="100%">Nitric Oxide Synthase Type III</style></keyword><keyword><style  face="normal" font="default" size="100%">NOS3 gene polymorphism</style></keyword><keyword><style  face="normal" font="default" size="100%">polymerase chain reaction</style></keyword><keyword><style  face="normal" font="default" size="100%">Polymorphism, Genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">Polymorphism, Restriction Fragment Length</style></keyword><keyword><style  face="normal" font="default" size="100%">restriction fragment length polymorphism</style></keyword><keyword><style  face="normal" font="default" size="100%">Risk factor</style></keyword><keyword><style  face="normal" font="default" size="100%">Risk Factors</style></keyword><keyword><style  face="normal" font="default" size="100%">variable number of tandem repeat</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2008</style></year><pub-dates><date><style  face="normal" font="default" size="100%">2008</style></date></pub-dates></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.scopus.com/inward/record.url?eid=2-s2.0-56049104685&amp;partnerID=40&amp;md5=a456112407dbb24c44b3689bcb78dbbb</style></url></web-urls></urls><volume><style face="normal" font="default" size="100%">63</style></volume><pages><style face="normal" font="default" size="100%">609 - 614</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Objective - Genetic polymorphisms in the gene for endothelial nitric oxide synthase have been considered as potential risk factors for the development of coronary artery disease in some populations. Methods - We studied two polymorphisms of the NOS3 gene, the VNTR in intron 4 (4VNTR) and the Glu298Asp polymorphism in exon 7, in relation to the existence of premature coronary artery disease and the occurrence of myocardial infarction. A total number of 370 individuals of the Greek population was examined by PCR-RFLP method. The patient group consisted of 209 subjects, aged less than 58 years presenting symptomatic coronary artery disease, documented by coronary angiography. Results - The frequencies for bb, ab and aa genotypes of 4VNTR polymorphism were 0.67, 0.29, 0.04, respectively, for the patient group and 0.73, 0.24, 0.03 for the control group. The frequencies for GG (Glu/ Glu), GT (Glu/Asp), TT (Asp/Asp) of the Glu298Asp polymorphism were 0.52, 0.41, 0.07, respectively, in patients compared to 0.47, 0.46, 0.07, in control subjects. Statistical analysis indicated that there are no significant differences in the frequencies of the genotypes between patients and control subjects for both polymorphisms. The combined analysis of the two polymorphisms indicated no synergistic effect of the a and T alleles on coronary artery disease. Conclusions - We have found no evidence for association between the a allele of the 4VNTR polymorphism, or the T allele of Glu298Asp polymorphism and the risk for premature coronary artery disease or occurrence of myocardial infarction. Furthermore, no synergistic contribution of these polymorphisms to the development of premature coronary artery disease has been observed.</style></abstract><issue><style face="normal" font="default" size="100%">5</style></issue><notes><style face="normal" font="default" size="100%">Cited By :16Export Date: 16 October 2015</style></notes></record></records></xml>