@article {6496, title = {Leaf tissue arrangement, preliminary phytochemical investigation and callus induction from the medicinal hemi-parasite Osyris alba L.}, journal = {International Journal of Pharmacognosy and Phytochemical Research}, volume = {8}, year = {2016}, note = {Export Date: 7 February 2017Correspondence Address: Mamoucha, S.; Department of Botany, Faculty of Biology, National and Kapodistrian University of AthensGreeceReferences: Woldemichael, G.M., Wink, M., Biomedical System 2010 Ecology, 30, pp. 139-140;Al-Jaber Hala, I., Mosleh, I.M., Mallouh, A., Abu Salim, O.M., Abu Zarga, M.H., Chemical constituents of Osyris alba and their antiparasitic activities (2010) Osyris alba, 12, pp. 814-820; Swamy, B.G.L., The comparative morphology of the Santalaceae: Node, secondary xylem, and pollen (1949) American Journal of Botany, 36, pp. 661-673; Metcalfe, C.R., Chalk, L., (1950) Anatomy of the Dicotyledons, 2, pp. 725-1500. , Clarendon Press, Oxford; Qasem, J.R., Host range of the parasitic weed (2006) Osyris alba, 6, pp. 74-78. , L. in Jordan Weed Biology Manag; Wanntorp, L., Ronse De Craene, L.P., Perianth evolution in the sandalwood order Santalales (2009) American Journal of Botany, 96, pp. 1361-1371; Joel, D.M., Kleifeld, Y., Bucsbaum, H., Osyris alba causing damage in orchards, pp. 378-381. , Proceedings of the 5th International Symposium of Parasitic Weeds (Nairobi, Kenya, 24-30 June 1991 CIMMYT, Nairobi; Woldemichael, G.M., Wink, M., (2002) Biomed. Syst. Ecol, 30, pp. 139-140; Subba, B., Basnet, P., Antimicrobial activity of some medicinal plants from east and central part of Nepal (2014) International Journal of Applied Science Biotechnology, 2, pp. 88-92; Mulaudzi, R.B., Ndhlala, A.R., Kulkarni, M.G., Finnie, J.F., Van Staden, J., Antimicrobial properties and phenolic contents of medicinal plants used by the Venda people for conditions related to venereal diseases (2011) Journal of Ethnopharmacology, 135, pp. 330-337; Girma, S., Mirutse, G., Berhanu, E., Hassen, M., Effect of crude leaf extract of Osyris quadripartita on Plasmodium berghei in Swiss albino mice (2015) BMC Complementary and Alternative Medicine, 15, p. 184; Hellwig, S., Drossard, J., Twyman, R.M., Fische, R., Plant cell cultures for the production of recombinant proteins (2004) Natural Biotechnology, 22, pp. 1415-1422; Barbulova, A., Fabio, A., Colucci, G., Review Plant Cell Cultures as Source of Cosmetic Active Ingredients Cosmetics (2014) Cosmetic, 1, pp. 94-104; Huang, Y., Tan, H., Guo, Z., Wu, X., Zhang, Q., Zhang, L., Diao, Y., The biosynthesis and genetic engineering of bioactive indole alkaloids in plants (2016) Journal of Plant Biology, 59, pp. 203-214; Wu, C.H., Dewir, Y.H., Hahn, E.J., Paek, K.Y., Optimization of culturing conditions for the production of biomass and phenolics from adventitious roots of Echinacea angustifolia (2006) Journal of Plant Biology, 49, pp. 193-195; Christodoulakis, N.S., Kogia, D., Mavroeidi, D., Fasseas, C., Anatomical and cytochemical investigation on the leaf of Teucrium polium L., a pharmaceutical shrub of the Greek phryganic formations (2010) Teucrium polium L., a pharmaceutical shrub of the Greek phryganic formations. Journal of Biological Research, 14, pp. 199-209; Christodoulakis, N.S., Lampri, P.N., Fasseas, C., Structural and cytochemical investigation on the leaf of silverleaf nightshade (Solanum eleagnifolium), a drought Resistant Alien Weed of the Greek Flora (2009) Australian Journal of Botany, 57, pp. 432-438; Murashige, T., Skoog, F., A revised medium for rapid growth and bio-assays with tobacco tissue cultures (1962) Physiology of Plant, 15, pp. 473-497; Coleman, W.K., Thorpe, T.A., In vitro culture of western redcedar (Thuja plicata Donn). I (1977) Plantlet formation Botany Gazette, 138, pp. 298-304; Jafari, N., Othman, R.Y., Khalid, N., Effect of benzylaminopurine (BAP) pulsing on in vitro shoot multiplication of Musa acuminata (banana) cv. Berangan (2011) African Journal of Biotechnology, 10, pp. 2446-2450; Record, S.J., Hess, R.W., (1943) Timbers of the New World, p. 640. , Yale University Press, New Haven; Bernardello, L.M., Estudios en Lycium (Solanaceae) I. Anatomia de la hoja y tallo, y sus diferencias con Grabowskia (1982) Boletin de la Sociedad Argentina de Botanica, 21, pp. 153-185; Cosa De Gastiazoro, M.T., Estudio morfoanatomico de organos vegetativos em Cestroideae (Solanaceae) (1994) III: Tribu Schwenckieae Kurtziana, 23, pp. 9-25; Fahn, A., Cutler, D.F., Xerophytes (1992) Encyclopedia of plant anatomy, 13; Terashima, I., Araya, T., Miyazawa, S.I., Sone, K., Yano, S., Construction and maintenance of the optical photosynthetic systems of the leaf, herbaceous plant and tree: An eco-developmental treatise (2005) Annals of Botany, 95, pp. 507-519; Rhizopoulou, S., Psaras, G.K., Development and structure of drought tolerant leaves of the Mediterranean shrub Capparis spinosa L (2003) Annals of Botany B, 92, pp. 377-383; Metcalfe, C.R., Chalk, L., (1957) Anatomy of the Dicotyledons II, , Claredon Press, Oxford; Mamoucha, S., Fokialakis, N., Christodoulakis, N.S., Leaf structure and histochemistry of Ficus carica (Moraceae), the fig tree (2016) Flora, 218, pp. 1-11; Rajni, N.P., Wood anatomy of the dicotyledons indigenous to New Zealand Santalaceae (1974) New Zealand Journal of Botany, 12, pp. 431-444; Al-Tardeh, S., Sawidis, T., Diannelidis, B.E., Delivopoulos, S., Morpho-anatomical features of the leaves of the mediterranean geophytes Urginea maritima (L) baker (Liliaceae) (2008) Journal of Plant Biology, 51, pp. 150-158; Franceschi, V.R., Horner, H.T., Calcium oxalate crystals in plants (1980) Botany Review, 46, pp. 361-427; Arnott, H.J., (1982) Three systems of biomineralization in plants with comments on the associated organic matrix, pp. 199-218. , Nancollas GH ed Biological mineralization and demineralization, Springer-Verlag, Berlin, Germany; Cot{\'e}, G.G., Diversity and distribution of idioblasts producing calcium oxalate crystals in Dieffenbachia seguine (Araceae) (2009) American Journal of Botany, 96, pp. 1245-1254; Thiago, P.C., Cleildo, P.S., Germano, V., Deysiane, O.B., Delcio, C.F., Medeiros, A.D., Dilma, M.B., Seasonal variation in the production of secondary metabolites and antimicrobial activity of two plant species used in Brazilian traditional medicine (2013) African Journal of Biotechology, 12, pp. 847-853 }, month = {2016}, pages = {1437 - 1443}, publisher = {International Journal of Pharmacognosy and Phytochemical Research}, abstract = {Osyris alba is a dioecious shrub used in traditional medicine for its bioactive secondary metabolites. It is used for its antiparasitic, antimicrobial and anti-bleeding properties. In the present study the leaf tissue arrangement, histochemsitry and callus formation of Osyris alba L. were investigated. Leaves (fresh and fixed) were observed by light, polarized oight and electron microscopy. Fresh stem was also observed by light microscopy. The sub-cellular localization of secondary metabolites was detected. Furthermore, in vitro production of its secondary metabolite, by using cell cultures, was also preformed. The compact leaves are amphistomatic, with a single layer of epidermal cells and contain idioblasts cells with crystals. They react positively to histochemical reagents for the major groups of secondary metabolites. In the preliminary histochemical screening, in vitro cultured cells also gave positive results. In conclusion, the medicinal nature of the plant is attributed to its phytochemical profile. Plant cell culture technologies can be used for the production of its bioactive molecules. {\textcopyright} 2016, International Journal of Pharmacognosy and Phytochemical Research. All rights reserved.}, keywords = {Callus, Histochemistry, Leaf anatomy, medicinal plant, Mediterranean}, isbn = {09754873 (ISSN)}, url = {https://www.scopus.com/inward/record.uri?eid=2-s2.0-84988699070\&partnerID=40\&md5=eacade54f591f439949d77e12ef6fa0a}, author = {Mamoucha, S. and Christodoulakis, N.S.} }