@article {469,
	title = {Molecular and immunological characterization of Mus a 5 allergen from banana fruit},
	journal = {Mol Nutr Food Res},
	volume = {56},
	number = {3},
	year = {2012},
	pages = {446-53},
	type = {Journal Article},
	abstract = {Banana fruit has become an important cause of fruit allergy in the recent years. Among the five registered IUIS allergens, Mus a 1 and Mus a 2 have been characterized in detail. In this study, molecular characterization and evaluation of the allergenic properties of β-1,3-glucanase from banana (Musa acuminata), denoted as Mus a 5, were performed.|The gene of Mus a 5 was cloned and sequenced. The obtained cDNA revealed a novel Mus a 5 isoform with an open reading frame encoding a protein of 340 amino acids comprising a putative signal peptide of 28 amino acid residues. By MALDI-TOF analysis Mus a 5 isolated from banana fruit revealed a molecular mass of 33451{\textpm}67 Da. Two Mus a 5 isoforms (pI 7.7 and 8.0) were detected by 2D immunoblot with an identical N-terminal sequence. By mass fingerprint, 76 and 83\% of the primary structure was confirmed for the two mature Mus a 5 isoforms, respectively. IgE reactivity to Mus a 5 was found in 74\% of patients sensitized to banana fruit. Upregulation of basophil activation markers CD63 and CD203c was achieved with Mus a 5 in a concentration-dependent manner.|Mus a 5 is a functional allergen and a candidate for the component-resolved allergy diagnosis of banana allergy.},
	keywords = {3(4)-beta-Glucanase Female Food Hypersensitivity Fruit Humans Immunoglobulin E Male Molecular Sequence Data Musa Phosphoric Diester Hydrolases Plant Proteins Protein Isoforms Pyrophosphatases Skin Tests Up-Regulation Young Adult, Adolescent Adult Allergens Amino Acid Sequence Antigens, CD63 Antigens, Molecular Endo-1, Plant Cloning},
	issn = {1613-4133},
	doi = {10.1002/mnfr.201100541},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/22162266},
	author = {Aleksic, I. and Popovic, M. and Dimitrijevic, R. and Andjelkovic, U. and Vassilopoulou, E. and Sinaniotis, A. and Atanaskovic-Markovic, M. and Lindner, B. and Petersen, A. and Papadopoulos, N. G. and Gavrovic-Jankulovic, M.}
}
