@article {612,
	title = {Budesonide and formoterol inhibit inflammatory mediator production by bronchial epithelial cells infected with rhinovirus},
	journal = {Clin Exp Allergy},
	volume = {39},
	number = {11},
	year = {2009},
	pages = {1700-10},
	type = {Journal Article},
	abstract = {Rhinoviruses (RVs) are responsible for the majority of acute asthma and chronic obstructive pulmonary disease (COPD) exacerbations. RVs infect the lower airways and induce the production of pro-inflammatory and remodelling-associated mediators. Budesonide (BUD) and formoterol (FORM) synergize in controlling asthma and COPD exacerbations; however, their effects on virus-induced inflammation and remodelling are less known.|We investigated whether BUD and FORM synergize in suppressing RV-induced inflammation and remodelling in the airways.|In vitro models of RV infection of BEAS-2B and primary normal human bronchial epithelial (NHBE) cells were used. We assessed the effects of individual and combined drugs administered post-infection, at a clinically relevant concentration range (10(-6)-10(-10) m), on the production of CCL5, CXCL10, CXCL8, IL-6 and the remodelling-associated VEGF and bFGF, using ELISA and RT-PCR.|BUD effectively suppressed RV-mediated induction of all mediators studied, in a concentration-dependent manner. FORM alone suppressed the production of CXCL8 and bFGF. The combination of BUD and FORM had concentration-dependent, additive or synergistic effects in the suppression of RV-induced CCL5, CXCL8 and CXCL10 in both cell types as well as VEGF in NHBE only. Combination treatment also resulted in an enhanced suppression of RV-induced IL-6, and CCL5 at the mRNA level as compared with BUD or FORM alone.|BUD and FORM suppress RV-induced chemokines and growth factors in bronchial epithelial cells in a concentration-dependent, synergistic or additive manner. These data further support the combined use of BUD and FORM in asthma and COPD and intensification of this therapy during exacerbations.},
	keywords = {Asthma Bronchi Bronchodilator Agents Budesonide Chemokine CXCL10 Chemokines Drug Synergism Epithelial Cells Ethanolamines Fibroblast Growth Factor 2 Humans Inflammation Inflammation Mediators Intercellular Adhesion Molecule-1 Picornaviridae Infections Pul, Chronic Obstructive Respiratory Mucosa Rhinovirus Vascular Endothelial Growth Factor A},
	issn = {1365-2222},
	doi = {10.1111/j.1365-2222.2009.03307.x},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/19549024},
	author = {Skevaki, C. L. and Christodoulou, I. and Spyridaki, I. S. and Tiniakou, I. and Georgiou, V. and Xepapadaki, P. and Kafetzis, D. A. and Papadopoulos, N. G.}
}
