@article {632,
	title = {Severe immediate allergic reactions to grapes: part of a lipid transfer protein-associated clinical syndrome},
	journal = {Int Arch Allergy Immunol},
	volume = {143},
	number = {2},
	year = {2007},
	pages = {92-102},
	type = {Journal Article},
	abstract = {Grape allergy is considered rare; grape lipid transfer protein (LTP; Vit v 1), an endochitinase and a thaumatin-like protein (TLP) have been reported as grape allergens. A considerable number of patients have referred to our department for severe reactions to grapes, and several IgE binding proteins were detected.|The aim of this study was to identify and characterise the allergens involved in severe allergic reactions to grapes and describe the population in which they occur.|Patients with reported severe allergic reactions to grapes (n = 37) are described. Grape allergens were purified/fractionated by a combination of chromatographic techniques, identified by proteomic analysis and biochemically characterised. Immunoreactivity was assessed by blot (inhibitions) and RAST (inhibitions), and skin prick tests were performed with the isolated allergens.|All subjects were polyallergic, sensitised and reactive to several additional foods and pollen. All patients were sensitised to grape LTP. A 28-kDa expansin, a 37.5-kDa polygalacturonase-inhibiting protein, a 39-kDa beta-1,3-glucanase and a 60-kDa protein were identified as minor grape allergens. Endochitinase and TLP did not play a role. Inhibition experiments revealed the possible cross-reactive role of LTP for clinical sensitivities to other LTP-containing plant foods, but also the involvement of cross-reactive carbohydrate determinants of minor allergens in IgE cross-reactivity.|LTP is the major grape allergen, while additional minor allergens may contribute to clinical reactivity. Severe grape allergy presents in atopic patients who frequently react to other LTP-containing, plant-derived foods. The {\textquoteright}LTP syndrome{\textquoteright} is the appropriate term to describe this condition.},
	keywords = {Adolescent Adult Antigens, Immediate Immunoglobulin E Male Proteomics Radioallergosorbent Test Vitis, Plant Blotting, Polyacrylamide Gel Female Food Hypersensitivity Humans Hypersensitivity, Preschool Electrophoresis, Western Carrier Proteins Child Child},
	issn = {1018-2438},
	doi = {10.1159/000098657},
	url = {http://www.ncbi.nlm.nih.gov/pubmed/17228170},
	author = {Vassilopoulou, E. and Zuidmeer, L. and Akkerdaas, J. and Tassios, I. and Rigby, N. R. and Mills, E. N. and van Ree, R. and Saxoni-Papageorgiou, P. and Papadopoulos, N. G.}
}
