<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Aleksic, I.</style></author><author><style face="normal" font="default" size="100%">Popovic, M.</style></author><author><style face="normal" font="default" size="100%">Dimitrijevic, R.</style></author><author><style face="normal" font="default" size="100%">Andjelkovic, U.</style></author><author><style face="normal" font="default" size="100%">Vassilopoulou, E.</style></author><author><style face="normal" font="default" size="100%">Sinaniotis, A.</style></author><author><style face="normal" font="default" size="100%">Atanaskovic-Markovic, M.</style></author><author><style face="normal" font="default" size="100%">Lindner, B.</style></author><author><style face="normal" font="default" size="100%">Petersen, A.</style></author><author><style face="normal" font="default" size="100%">Papadopoulos, N. G.</style></author><author><style face="normal" font="default" size="100%">Gavrovic-Jankulovic, M.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Molecular and immunological characterization of Mus a 5 allergen from banana fruit</style></title><secondary-title><style face="normal" font="default" size="100%">Mol Nutr Food Res</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">3(4)-beta-Glucanase Female Food Hypersensitivity Fruit Humans Immunoglobulin E Male Molecular Sequence Data Musa Phosphoric Diester Hydrolases Plant Proteins Protein Isoforms Pyrophosphatases Skin Tests Up-Regulation Young Adult</style></keyword><keyword><style  face="normal" font="default" size="100%">Adolescent Adult Allergens Amino Acid Sequence Antigens</style></keyword><keyword><style  face="normal" font="default" size="100%">CD63 Antigens</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular Endo-1</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Cloning</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2012</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/22162266</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">3</style></number><volume><style face="normal" font="default" size="100%">56</style></volume><pages><style face="normal" font="default" size="100%">446-53</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Banana fruit has become an important cause of fruit allergy in the recent years. Among the five registered IUIS allergens, Mus a 1 and Mus a 2 have been characterized in detail. In this study, molecular characterization and evaluation of the allergenic properties of β-1,3-glucanase from banana (Musa acuminata), denoted as Mus a 5, were performed.|The gene of Mus a 5 was cloned and sequenced. The obtained cDNA revealed a novel Mus a 5 isoform with an open reading frame encoding a protein of 340 amino acids comprising a putative signal peptide of 28 amino acid residues. By MALDI-TOF analysis Mus a 5 isolated from banana fruit revealed a molecular mass of 33451±67 Da. Two Mus a 5 isoforms (pI 7.7 and 8.0) were detected by 2D immunoblot with an identical N-terminal sequence. By mass fingerprint, 76 and 83% of the primary structure was confirmed for the two mature Mus a 5 isoforms, respectively. IgE reactivity to Mus a 5 was found in 74% of patients sensitized to banana fruit. Upregulation of basophil activation markers CD63 and CD203c was achieved with Mus a 5 in a concentration-dependent manner.|Mus a 5 is a functional allergen and a candidate for the component-resolved allergy diagnosis of banana allergy.</style></abstract><work-type><style face="normal" font="default" size="100%">Journal Article</style></work-type></record></records></xml>