<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Vassilopoulou, E.</style></author><author><style face="normal" font="default" size="100%">Zuidmeer, L.</style></author><author><style face="normal" font="default" size="100%">Akkerdaas, J.</style></author><author><style face="normal" font="default" size="100%">Tassios, I.</style></author><author><style face="normal" font="default" size="100%">Rigby, N. R.</style></author><author><style face="normal" font="default" size="100%">Mills, E. N.</style></author><author><style face="normal" font="default" size="100%">van Ree, R.</style></author><author><style face="normal" font="default" size="100%">Saxoni-Papageorgiou, P.</style></author><author><style face="normal" font="default" size="100%">Papadopoulos, N. G.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Severe immediate allergic reactions to grapes: part of a lipid transfer protein-associated clinical syndrome</style></title><secondary-title><style face="normal" font="default" size="100%">Int Arch Allergy Immunol</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Adolescent Adult Antigens</style></keyword><keyword><style  face="normal" font="default" size="100%">Immediate Immunoglobulin E Male Proteomics Radioallergosorbent Test Vitis</style></keyword><keyword><style  face="normal" font="default" size="100%">Plant Blotting</style></keyword><keyword><style  face="normal" font="default" size="100%">Polyacrylamide Gel Female Food Hypersensitivity Humans Hypersensitivity</style></keyword><keyword><style  face="normal" font="default" size="100%">Preschool Electrophoresis</style></keyword><keyword><style  face="normal" font="default" size="100%">Western Carrier Proteins Child Child</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2007</style></year></dates><urls><web-urls><url><style face="normal" font="default" size="100%">http://www.ncbi.nlm.nih.gov/pubmed/17228170</style></url></web-urls></urls><number><style face="normal" font="default" size="100%">2</style></number><volume><style face="normal" font="default" size="100%">143</style></volume><pages><style face="normal" font="default" size="100%">92-102</style></pages><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Grape allergy is considered rare; grape lipid transfer protein (LTP; Vit v 1), an endochitinase and a thaumatin-like protein (TLP) have been reported as grape allergens. A considerable number of patients have referred to our department for severe reactions to grapes, and several IgE binding proteins were detected.|The aim of this study was to identify and characterise the allergens involved in severe allergic reactions to grapes and describe the population in which they occur.|Patients with reported severe allergic reactions to grapes (n = 37) are described. Grape allergens were purified/fractionated by a combination of chromatographic techniques, identified by proteomic analysis and biochemically characterised. Immunoreactivity was assessed by blot (inhibitions) and RAST (inhibitions), and skin prick tests were performed with the isolated allergens.|All subjects were polyallergic, sensitised and reactive to several additional foods and pollen. All patients were sensitised to grape LTP. A 28-kDa expansin, a 37.5-kDa polygalacturonase-inhibiting protein, a 39-kDa beta-1,3-glucanase and a 60-kDa protein were identified as minor grape allergens. Endochitinase and TLP did not play a role. Inhibition experiments revealed the possible cross-reactive role of LTP for clinical sensitivities to other LTP-containing plant foods, but also the involvement of cross-reactive carbohydrate determinants of minor allergens in IgE cross-reactivity.|LTP is the major grape allergen, while additional minor allergens may contribute to clinical reactivity. Severe grape allergy presents in atopic patients who frequently react to other LTP-containing, plant-derived foods. The 'LTP syndrome' is the appropriate term to describe this condition.</style></abstract><work-type><style face="normal" font="default" size="100%">Journal Article</style></work-type></record></records></xml>