@article {36596,
	title = {Evidence for a dimeric assembly of two titin/telethonin complexes induced by the telethonin C-terminus},
	journal = {J Struct BiolJ Struct Biol},
	volume = {155},
	number = {2},
	year = {2006},
	note = {Pinotsis, NikosPetoukhov, MaximLange, StephanSvergun, DmitriZou, PeijianGautel, MathiasWilmanns, MatthiasengG0200496/MRC_/Medical Research Council/United KingdomResearch Support, Non-U.S. Gov{\textquoteright}tJ Struct Biol. 2006 Aug;155(2):239-50. doi: 10.1016/j.jsb.2006.03.028. Epub 2006 Apr 27.},
	month = {Aug},
	pages = {239-50},
	edition = {2006/05/23},
	abstract = {The Z-disk region defines the lateral boundary of the sarcomere and requires a high level of mechanical strength to provide a stable framework for large filamentous muscle proteins. The level of complexity at this area is reflected by a large number of protein-protein interactions. Recently, we unraveled how the N-terminus of the longest filament component, the giant muscle protein titin, is assembled into an antiparallel (2:1) sandwich complex by the N-terminal titin-binding segment of the Z-disk ligand telethonin/T-cap [Zou, P., Pinotsis, N., Lange, S., Song, Y.H., Popov, A., Mavridis, I., Mayans, O.M., Gautel, M., Wilmanns, M., 2006. Palindromic assembly of the giant muscle protein titin in the sarcomeric Z-disk. Nature 439, 229-233]. In this contribution, we present structural data of a related complex of the titin N-terminus with full-length telethonin. The C-terminus of telethonin remains invisible, suggesting that it does not fold into a defined structure even in the presence of titin. In contrast to the structure with truncated telethonin, a dimer of two titin/telethonin complexes is formed within the crystal environment, potentially indicating the formation of higher oligomers. We further investigated the structure and dynamics of this assembly by small-angle X-ray scattering, circular dichroism, and in vivo complementation data. The data consistently indicate the involvement of the C-terminal part of telethonin into the assembly of two titin/telethonin complexes.},
	keywords = {Animals, Chlorocebus aethiops, Circular Dichroism/methods, Connectin, COS Cells, Crystallization/methods, Crystallography, X-Ray/methods, Dimerization, Genetic Complementation Test, Humans, Immunoblotting, Microscopy, Confocal/methods, Muscle Proteins/chemistry/genetics/*metabolism, Myocytes, Cardiac/metabolism, Peptide Fragments/chemistry/genetics/*metabolism, Protein Binding, Protein Kinases/genetics/*metabolism, Protein Structure, Secondary, Protein Structure, Tertiary, Rats, Sarcomeres/metabolism},
	isbn = {1047-8477 (Print)1047-8477 (Linking)},
	author = {Pinotsis, N. and Petoukhov, M. and Lange, S. and Svergun, D. and Zou, P. and Gautel, M. and Wilmanns, M.}
}
