@article {36597,
	title = {Crystal structure of the Legionella pneumophila Lpg2936 in complex with the cofactor S-adenosyl-L-methionine reveals novel insights into the mechanism of RsmE family methyltransferases},
	journal = {Protein SciProtein Sci},
	volume = {26},
	number = {12},
	year = {2017},
	note = {Pinotsis, NikosWaksman, Gabrieleng321630/ERC_/European Research Council/InternationalProtein Sci. 2017 Dec;26(12):2381-2391. doi: 10.1002/pro.3305. Epub 2017 Oct 27.},
	month = {Dec},
	pages = {2381-2391},
	edition = {2017/09/25},
	abstract = {The methylation of U1498 located in the 16S ribosomal RNA of Escherichia coli is an important modification affecting ribosomal activity. RsmE methyltransferases methylate specifically this position in a mechanism that requires an S-adenosyl-L-methionine (AdoMet) molecule as cofactor. Here we report the structure of Apo and AdoMet-bound Lpg2936 from Legionella pneumophila at 1.5 and 2.3 A, respectively. The protein comprises an N-terminal PUA domain and a C-terminal SPOUT domain. The latter is responsible for protein dimerization and cofactor binding. Comparison with similar structures suggests that Lpg2936 is an RsmE-like enzyme that can target the equivalent of U1498 in the L. pneumophila ribosomal RNA, thereby potentially enhancing ribosomal activity during infection-mediated effector production. The multiple copies of the enzyme found in both structures reveal a flexible conformation of the bound AdoMet ligand. Isothermal titration calorimetry measurements suggest an asymmetric two site binding mode. Our results therefore also provide unprecedented insights into AdoMet/RsmE interaction, furthering our understanding of the RsmE catalytic mechanism.},
	keywords = {Bacterial Proteins/*chemistry/metabolism, Binding Sites, Crystallization, Legionella pneumophila, Legionella pneumophila/*enzymology, Methyltransferases/*chemistry/metabolism, Models, Molecular, Protein Binding, RNA methylation, RNA, Bacterial/chemistry/metabolism, RsmE methyltransferase, S-adenosyl-L-methionine, S-Adenosylmethionine/*chemistry/metabolism, trefoil knot},
	isbn = {1469-896X (Electronic)0961-8368 (Print)0961-8368 (Linking)},
	author = {Pinotsis, N. and Waksman, G.}
}
