@article {171,
	title = {Distinct pools of beta-amyloid in Alzheimer disease-affected brain: a clinicopathologic study.},
	journal = {Arch Neurol},
	volume = {65},
	year = {2008},
	month = {2008 Jul},
	pages = {906-12},
	abstract = {OBJECTIVE: To determine whether beta-amyloid (Abeta) peptides segregated into distinct biochemical compartments would differentially correlate with clinical severity of Alzheimer disease (AD).

DESIGN: Clinicopathologic correlation study.

PARTICIPANTS: Twenty-seven patients from a longitudinal study of AD and 13 age- and sex-matched controls without a known history of cognitive impairment or dementia were included in this study.

INTERVENTIONS: Temporal and cingulate neocortex were processed using a 4-step extraction, yielding biochemical fractions that are hypothesized to be enriched with proteins from distinct anatomical compartments: TRIS (extracellular soluble), Triton (intracellular soluble), sodium dodecyl sulfate (SDS) (membrane associated), and formic acid (extracellular insoluble). Levels of Abeta(40) and Abeta(42) were quantified in each biochemical compartment by enzyme-linked immunosorbent assay.

RESULTS: The Abeta(42) level in all biochemical compartments was significantly elevated in patients with AD vs controls (P < .01). The Abeta(40) levels in the TRIS and formic acid fractions were elevated in patients with AD (temporal, P < .01; cingulate, P = .03); however, Triton and SDS Abeta(40) levels were similar in patients with AD and in controls. Functional impairment proximal to death correlated with Triton Abeta(42) (r = 0.48, P = .02) and SDS Abeta(42) (r = 0.41, P = .04) in the temporal cortex. Faster cognitive decline was associated with elevated temporal SDS Abeta(42) levels (P < .001), whereas slower decline was associated with elevated cingulate formic acid Abeta(42) and SDS Abeta(42) levels (P = .02 and P = .01, respectively).

CONCLUSION: Intracellular and membrane-associated Abeta, especially Abeta(42) in the temporal neocortex, may be more closely related to AD symptoms than other measured Abeta species.},
	keywords = {Aged, Aged, 80 and over, Alzheimer Disease, Amyloid beta-Peptides, Brain, Brief Psychiatric Rating Scale, Cross-Sectional Studies, Female, Humans, Longitudinal Studies, Male, Middle Aged, Plaque, Amyloid},
	issn = {1538-3687},
	doi = {10.1001/archneur.65.7.906},
	author = {Steinerman, Joshua R and Irizarry, Michael and Scarmeas, Nikolaos and Raju, Susan and Brandt, Jason and Albert, Marilyn and Blacker, Deborah and Hyman, Bradley and Stern, Yaakov}
}
