<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Fang, E. F.</style></author><author><style face="normal" font="default" size="100%">Palikaras, K.</style></author><author><style face="normal" font="default" size="100%">Sun, N.</style></author><author><style face="normal" font="default" size="100%">Fivenson, E. M.</style></author><author><style face="normal" font="default" size="100%">Spangler, R. D.</style></author><author><style face="normal" font="default" size="100%">Kerr, J. S.</style></author><author><style face="normal" font="default" size="100%">Cordonnier, S. A.</style></author><author><style face="normal" font="default" size="100%">Hou, Y.</style></author><author><style face="normal" font="default" size="100%">Dombi, E.</style></author><author><style face="normal" font="default" size="100%">Kassahun, H.</style></author><author><style face="normal" font="default" size="100%">Tavernarakis, N.</style></author><author><style face="normal" font="default" size="100%">Poulton, J.</style></author><author><style face="normal" font="default" size="100%">Nilsen, H.</style></author><author><style face="normal" font="default" size="100%">Bohr, V. A.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">In Vitro and In Vivo Detection of Mitophagy in Human Cells, C. Elegans, and Mice</style></title><secondary-title><style face="normal" font="default" size="100%">J Vis Exp</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Caenorhabditis elegans Proteins/*metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Caenorhabditis elegans/*metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Female</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Male</style></keyword><keyword><style  face="normal" font="default" size="100%">Mice</style></keyword><keyword><style  face="normal" font="default" size="100%">Mitochondria/*metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Mitophagy/*physiology</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2017</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Nov 22</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">129</style></number><edition><style face="normal" font="default" size="100%">2017/12/30</style></edition><isbn><style face="normal" font="default" size="100%">1940-087X (Electronic)1940-087X (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Mitochondria are the powerhouses of cells and produce cellular energy in the form of ATP. Mitochondrial dysfunction contributes to biological aging and a wide variety of disorders including metabolic diseases, premature aging syndromes, and neurodegenerative diseases such as Alzheimer's disease (AD) and Parkinson's disease (PD). Maintenance of mitochondrial health depends on mitochondrial biogenesis and the efficient clearance of dysfunctional mitochondria through mitophagy. Experimental methods to accurately detect autophagy/mitophagy, especially in animal models, have been challenging to develop. Recent progress towards the understanding of the molecular mechanisms of mitophagy has enabled the development of novel mitophagy detection techniques. Here, we introduce several versatile techniques to monitor mitophagy in human cells, Caenorhabditis elegans (e.g., Rosella and DCT-1/ LGG-1 strains), and mice (mt-Keima). A combination of these mitophagy detection techniques, including cross-species evaluation, will improve the accuracy of mitophagy measurements and lead to a better understanding of the role of mitophagy in health and disease.</style></abstract><accession-num><style face="normal" font="default" size="100%">29286376</style></accession-num><notes><style face="normal" font="default" size="100%">Fang, Evandro FPalikaras, KonstantinosSun, NuoFivenson, Elayne MSpangler, Ryan DKerr, Jesse SCordonnier, Stephanie AHou, YujunDombi, EszterKassahun, HenokTavernarakis, NektariosPoulton, JoannaNilsen, HildeBohr, Vilhelm AengMR/J010448/1/Medical Research Council/United KingdomZ99 AG999999/Intramural NIH HHS/Research Support, N.I.H., IntramuralResearch Support, Non-U.S. Gov'tVideo-Audio MediaJ Vis Exp. 2017 Nov 22;(129). doi: 10.3791/56301.</style></notes><custom2><style face="normal" font="default" size="100%">PMC5755444</style></custom2><auth-address><style face="normal" font="default" size="100%">Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health; Department of Clinical Molecular Biology, University of Oslo and Akershus University Hospital; evandrofeifang@yahoo.com; e.f.fang@medisin.uio.no.Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology - Hellas.Center for Molecular Medicine, National Heart Lung and Blood Institute, National Institutes of Health.Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health.Laboratory of Neurosciences, National Institute on Aging, National Institutes of Health.Nuffield Department of Obstetrics and Gynaecology, University of Oxford.Department of Clinical Molecular Biology, University of Oslo and Akershus University Hospital.Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology - Hellas; Department of Basic Sciences, Faculty of Medicine, University of Crete.Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health; Danish Center for Healthy Aging, University of Copenhagen; BohrV@grc.nia.nih.gov.</style></auth-address></record></records></xml>