<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Palikaras, K.</style></author><author><style face="normal" font="default" size="100%">Lionaki, E.</style></author><author><style face="normal" font="default" size="100%">Tavernarakis, N.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Mitophagy Dynamics in Caenorhabditis elegans</style></title><secondary-title><style face="normal" font="default" size="100%">Methods Mol Biol</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">*Aging</style></keyword><keyword><style  face="normal" font="default" size="100%">*Autophagosome</style></keyword><keyword><style  face="normal" font="default" size="100%">*Autophagy</style></keyword><keyword><style  face="normal" font="default" size="100%">*Caenorhabditis elegans</style></keyword><keyword><style  face="normal" font="default" size="100%">*DsRed</style></keyword><keyword><style  face="normal" font="default" size="100%">*Fluorescent microscopy</style></keyword><keyword><style  face="normal" font="default" size="100%">*Green Fluorescent Protein (GFP)</style></keyword><keyword><style  face="normal" font="default" size="100%">*Lysosomes</style></keyword><keyword><style  face="normal" font="default" size="100%">*Mitochondria</style></keyword><keyword><style  face="normal" font="default" size="100%">*Mitophagy</style></keyword><keyword><style  face="normal" font="default" size="100%">*mtRosella</style></keyword><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Animals, Genetically Modified</style></keyword><keyword><style  face="normal" font="default" size="100%">Biological Assay/instrumentation/*methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Caenorhabditis elegans Proteins/genetics/metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Caenorhabditis elegans/physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Electron Transport Complex III/genetics/metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Genes, Reporter/genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Image Processing, Computer-Assisted/instrumentation/methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Intravital Microscopy/instrumentation/*methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Luminescent Proteins/genetics/metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Microscopy, Fluorescence/instrumentation/methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Microtubule-Associated Proteins/genetics/metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Mitochondria/metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Mitophagy/*physiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Models, Animal</style></keyword><keyword><style  face="normal" font="default" size="100%">Muscles/cytology/metabolism</style></keyword><keyword><style  face="normal" font="default" size="100%">Neurons/metabolism</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2019</style></year></dates><edition><style face="normal" font="default" size="100%">2019/01/06</style></edition><volume><style face="normal" font="default" size="100%">1880</style></volume><pages><style face="normal" font="default" size="100%">655-668</style></pages><isbn><style face="normal" font="default" size="100%">1940-6029 (Electronic)1064-3745 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Mitochondrial selective autophagy (mitophagy) is a critical cellular process for mitochondrial homeostasis and survival both under basal and stress conditions. Distinct cell types display different requirements for mitochondrial turnover depending on their metabolic status, differentiation state, and environmental cues. This points to the necessity of developing novel tools for real-time, tissue-specific assessment of mitophagy. Caenorhabditis elegans is an invaluable model organism for this kind of analysis providing a platform for simultaneous monitoring of mitophagy in vivo in different tissues and cell types, during development, stress conditions, and/or throughout life span. In this chapter we describe three versatile, noninvasive methods, developed for monitoring in vivo early and late mitophagic events in body wall muscles and neuronal cells of C. elegans. These procedures can be readily used and/or provide insights into the generation of novel imaging methods to investigate further the role of mitophagy at the organismal level under normal and pathological conditions.</style></abstract><accession-num><style face="normal" font="default" size="100%">30610729</style></accession-num><notes><style face="normal" font="default" size="100%">Palikaras, KonstantinosLionaki, EiriniTavernarakis, NektariosengResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tMethods Mol Biol. 2019;1880:655-668. doi: 10.1007/978-1-4939-8873-0_43.</style></notes><auth-address><style face="normal" font="default" size="100%">Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece.Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology Hellas, Crete, Greece. tavernarakis@imbb.forth.gr.Faculty of Medicine, Department of Basic Sciences, University of Crete, Crete, Greece. tavernarakis@imbb.forth.gr.</style></auth-address></record></records></xml>