<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Palikaras, K.</style></author><author><style face="normal" font="default" size="100%">SenGupta, T.</style></author><author><style face="normal" font="default" size="100%">Nilsen, H.</style></author><author><style face="normal" font="default" size="100%">Tavernarakis, N.</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Assessment of dopaminergic neuron degeneration in a C. elegans model of Parkinson's disease</style></title><secondary-title><style face="normal" font="default" size="100%">STAR Protoc</style></secondary-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">*Caenorhabditis elegans Proteins</style></keyword><keyword><style  face="normal" font="default" size="100%">*Cell Biology</style></keyword><keyword><style  face="normal" font="default" size="100%">*Model Organisms</style></keyword><keyword><style  face="normal" font="default" size="100%">*Molecular Biology</style></keyword><keyword><style  face="normal" font="default" size="100%">*Neuroscience</style></keyword><keyword><style  face="normal" font="default" size="100%">*Parkinson Disease/genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Animals</style></keyword><keyword><style  face="normal" font="default" size="100%">Caenorhabditis elegans</style></keyword><keyword><style  face="normal" font="default" size="100%">Disease Models, Animal</style></keyword><keyword><style  face="normal" font="default" size="100%">Dopamine</style></keyword><keyword><style  face="normal" font="default" size="100%">Dopaminergic Neurons</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2022</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jun 17</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">2</style></number><edition><style face="normal" font="default" size="100%">2022/04/12</style></edition><volume><style face="normal" font="default" size="100%">3</style></volume><pages><style face="normal" font="default" size="100%">101264</style></pages><isbn><style face="normal" font="default" size="100%">2666-1667 (Electronic)2666-1667 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Transgenic Caenorhabditis elegans that expresses the full-length wild-type human alpha-synuclein in dopaminergic neurons provides a well-established Parkinson's disease (PD) nematode model. Here, we present a detailed protocol to monitor and dissect the molecular underpinnings of age-associated neurodegeneration using this PD nematode model. This protocol includes preparation of nematode growth media and bacterial food sources, as well as procedures for nematode growth, synchronization, and treatment. We then describe procedures to assess dopaminergic neuronal death in vivo using fluorescence imaging. For complete details on the use and execution of this protocol, please refer to SenGupta et al. (2021).</style></abstract><accession-num><style face="normal" font="default" size="100%">35403008</style></accession-num><notes><style face="normal" font="default" size="100%">Palikaras, KonstantinosSenGupta, TanimaNilsen, HildeTavernarakis, NektariosengResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tSTAR Protoc. 2022 Mar 31;3(2):101264. doi: 10.1016/j.xpro.2022.101264. eCollection 2022 Jun 17.</style></notes><custom2><style face="normal" font="default" size="100%">PMC8983426</style></custom2><auth-address><style face="normal" font="default" size="100%">Department of Physiology, School of Medicine, National and Kapodistrian University of Athens, 11527 Athens, Greece.Department of Clinical Molecular Biology, University of Oslo, Oslo, Norway.Department of Clinical Molecular Biology, Akershus University Hospital, Lorenskog, Norway.Institute of Molecular Biology and Biotechnology, Foundation for Research and Technology-Hellas, Heraklion, 71300 Crete, Greece.Department of Basic Sciences, Faculty of Medicine, University of Crete, Heraklion, 71300 Crete, Greece.</style></auth-address></record></records></xml>