@article {IECB2023-14583,
	title = {Immunosensing Cancer Markers through Surface-Enhanced Photoluminescence on Nanostructured Silver Substrates},
	journal = {Engineering Proceedings},
	volume = {35},
	number = {1},
	year = {2023},
	abstract = {Noble metal nanostructured substrates enhance photoluminescence emitted from molecules immobilized onto their surface, allowing for the development of highly sensitive immunoassays employing fluorescent labels. In this work, nanostructured silver surfaces were implemented as substrates for the immunochemical detection of two ovarian cancer markers, carbohydrate antigen 125 (CA125) and human epididymis protein 4 (HE4). Biotinylated detection antibodies were used to allow for the detection of immunocomplexes through a reaction with streptavidin conjugated to Rhodamine Red-X. The detection limits achieved were 2.5 U/mL and 0.06 ng/mL for CA125 and HE4, respectively, with linear dynamic ranges, covering the concentration ranges of both healthy and ovarian cancer patients.},
	issn = {2673-4591},
	doi = {10.3390/IECB2023-14583},
	url = {https://www.mdpi.com/2673-4591/35/1/7},
	author = {Geka, Georgia and Kanioura, Anastasia and Kochylas, Ioannis and Likodimos, Vlassis and Spiros Gardelis and Chatzantonaki, Kalliopi and Charvalos, Ekaterina and Dimitriou, Anastasios and Papanikolaou, Nikolaos and Economou, Anastasios and Kakabakos, Sotirios and Petrou, Panagiota}
}
