@article {proceedings2024104019,
	title = {Superoxide Dismutase Determination on Silver Nanostructured Substrates through Surface-Enhanced Photoluminescence},
	journal = {Proceedings},
	volume = {104},
	number = {1},
	year = {2024},
	abstract = {Oxidative stress is defined by an imbalance between the generation of reactive oxygen species and the biological system{\textquoteright}s ability to neutralize them. This condition is commonly linked to various pathological conditions [1]. Superoxide dismutase (SOD) is a widely used enzyme to assess oxidative stress, and various techniques have been developed for its detection in biological samples such as blood, urine, and saliva [2]. Surface-enhanced photoluminescence (PL) is a particularly sensitive method, offering minimal interference from the sample matrix [3]. In this work, silver nanostructured surfaces were implemented as substrates for the immunochemical determination of SOD in synthetic saliva through PL. The substrates were prepared using a single-step metal-assisted chemical etching method (MACE), resulting in the formation of silicon nanowires decorated with silver dendrites of approximately 1.5 μm in height [4]. For SOD detection, a three-step competitive immunoassay configuration was followed. Briefly, SOD was immobilized onto the substrates and then the functionalized substrates were incubated with mixtures of SOD with anti-SOD primary antibody, prepared either in assay buffer or synthetic saliva. Then, a solution of biotinylated anti-species specific antibody was added, followed by a reaction with streptavidin labelled with the fluorescent dye Rhodamine Red-X, and the signal was determined through an in-house developed optical set-up. The developed method presents similar or slightly lower sensitivity (detection limit 0.05 μg/mL) compared to the literature; however, it does not require labor-intensive sample pretreatment steps [5,6]. The aforementioned findings demonstrate the capability of the developed method to detect superoxide dismutase in natural saliva, in order to evaluate the oxidative stress status of an organism.},
	issn = {2504-3900},
	doi = {10.3390/proceedings2024104019},
	url = {https://www.mdpi.com/2504-3900/104/1/19},
	author = {Kanioura, Anastasia and Geka, Georgia and Kochylas, Ioannis and Likodimos, Vlassis and Spiros Gardelis and Dimitriou, Anastasios and Papanikolaou, Nikolaos and Kakabakos, Sotirios and Petrou, Panagiota}
}
