<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Paraskevis, D</style></author><author><style face="normal" font="default" size="100%">Beloukas, A.</style></author><author><style face="normal" font="default" size="100%">Haida, C</style></author><author><style face="normal" font="default" size="100%">Katsoulidou, A</style></author><author><style face="normal" font="default" size="100%">Moschidis, Z.</style></author><author><style face="normal" font="default" size="100%">Hatzitheodorou, H.</style></author><author><style face="normal" font="default" size="100%">Varaklioti, A.</style></author><author><style face="normal" font="default" size="100%">Sypsa, V</style></author><author><style face="normal" font="default" size="100%">Hatzakis, A</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Development of a new ultra sensitive real-time PCR assay (ultra sensitive RTQ-PCR) for the quantification of HBV-DNA</style></title><secondary-title><style face="normal" font="default" size="100%">Virol JVirol JVirol J</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Virology journal</style></alt-title><short-title><style face="normal" font="default" size="100%">Virology journalVirology journal</style></short-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">DNA, Viral/*blood</style></keyword><keyword><style  face="normal" font="default" size="100%">Hepatitis B virus/genetics/*isolation &amp; purification</style></keyword><keyword><style  face="normal" font="default" size="100%">Hepatitis B/*diagnosis</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Polymerase Chain Reaction/*methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Sensitivity and Specificity</style></keyword><keyword><style  face="normal" font="default" size="100%">Viral Load/*methods</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2010</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Mar 12</style></date></pub-dates></dates><volume><style face="normal" font="default" size="100%">7</style></volume><pages><style face="normal" font="default" size="100%">57</style></pages><isbn><style face="normal" font="default" size="100%">1743-422X (Electronic)1743-422X (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">BACKGROUND: Improved sensitivity of HBV-DNA tests is of critical importance for the management of HBV infection. Our aim was to develop and assess a new ultra sensitive in-house real-time PCR assay for HBV-DNA quantification (ultra sensitive RTQ-PCR). RESULTS: Previously used HBV-DNA standards were calibrated against the WHO 1st International Standard for HBV-DNA (OptiQuant(R) HBV-DNA Quantification Panel, Accrometrix Europe B.V.). The 95% and 50% HBV-DNA detection end-point of the assay were 22.2 and 8.4 IU/mL. According to the calibration results, 1 IU/mL equals 2.8 copies/mL. Importantly the clinical performance of the ultra sensitive real-time PCR was tested similar (67%) to the Procleix Ultrio discriminatory HBV test (dHBV) (70%) in low-titer samples from patients with occult Hepatitis B. Finally, in the comparison of ultra sensitive RTQ-PCR with the commercially available COBAS TaqMan HBV Test, the in-house assay identified 94.7% of the 94 specimens as positive versus 90.4% identified by TaqMan, while the quantitative results that were positive by both assay were strongly correlated (r = 0.979). CONCLUSIONS: We report a new ultra sensitive real time PCR molecular beacon based assay with remarkable analytical and clinical sensitivity, calibrated against the WHO 1st International standard.</style></abstract><accession-num><style face="normal" font="default" size="100%">20226057</style></accession-num><notes><style face="normal" font="default" size="100%">Paraskevis, DimitriosBeloukas, ApostolosHaida, CatherineKatsoulidou, AntigoniMoschidis, ZisisHatzitheodorou, HelenVaraklioti, AgoritsaSypsa, VanaHatzakis, AngelosengComparative StudyEvaluation StudyResearch Support, Non-U.S. Gov'tEngland2010/03/17 06:00Virol J. 2010 Mar 12;7:57. doi: 10.1186/1743-422X-7-57.</style></notes><custom2><style face="normal" font="default" size="100%">2848214</style></custom2><auth-address><style face="normal" font="default" size="100%">Department of Hygiene Epidemiology and Medical Statistics, Medical School, University of Athens, Athens, Greece. dparask@med.uoa.gr</style></auth-address></record></records></xml>