<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Beloukas, A.</style></author><author><style face="normal" font="default" size="100%">Paraskevis, D</style></author><author><style face="normal" font="default" size="100%">Haida, C</style></author><author><style face="normal" font="default" size="100%">Sypsa, V</style></author><author><style face="normal" font="default" size="100%">Hatzakis, A</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Development and assessment of a multiplex real-time PCR assay for quantification of human immunodeficiency virus type 1 DNA</style></title><secondary-title><style face="normal" font="default" size="100%">J Clin MicrobiolJournal of clinical microbiologyJournal of clinical microbiology</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Journal of clinical microbiology</style></alt-title><short-title><style face="normal" font="default" size="100%">J Clin MicrobiolJ Clin Microbiol</style></short-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">DNA, Viral/*blood/*genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">HIV Infections/*virology</style></keyword><keyword><style  face="normal" font="default" size="100%">HIV-1/genetics/*isolation &amp; purification</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Polymerase Chain Reaction/*methods</style></keyword><keyword><style  face="normal" font="default" size="100%">Sensitivity and Specificity</style></keyword><keyword><style  face="normal" font="default" size="100%">Viral Load/*methods</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2009</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jul</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">7</style></number><volume><style face="normal" font="default" size="100%">47</style></volume><pages><style face="normal" font="default" size="100%">2194-9</style></pages><isbn><style face="normal" font="default" size="100%">1098-660X (Electronic)0095-1137 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">Previous studies showed that high levels of human immunodeficiency virus type 1 (HIV-1) DNA are associated with a faster progression to AIDS, an increased risk of death, and a higher risk of HIV RNA rebound in patients on highly active antiretroviral therapy. Our objective was to develop and assess a highly sensitive real-time multiplex PCR assay for the quantification of HIV-1 DNA (RTMP-HIV) based on molecular beacons. HIV-1 DNA quantification was carried out by RTMP in a LightCycler 2.0 apparatus. HIV-1 DNA was quantified in parallel with CCR5 as a reference gene, and reported values are numbers of HIV-1 DNA copies/10(6) peripheral blood mononuclear cells (PBMCs). The clinical sensitivity of the assay was assessed for 115 newly diagnosed HIV-1-infected individuals. The analytical sensitivity was estimated to be 12.5 copies of HIV-1 DNA per 10(6) PBMCs, while the clinical sensitivity was 100%, with levels ranging from 1.23 to 4.25 log(10) HIV-1 DNA copies/10(6) PBMCs. In conclusion, we developed and assessed a new RTMP-HIV assay based on molecular beacons, using a LightCycler 2.0 instrument. This multiplex assay has comparable sensitivity, reproducibility, and accuracy to single real-time PCR assays.</style></abstract><accession-num><style face="normal" font="default" size="100%">19420173</style></accession-num><notes><style face="normal" font="default" size="100%">Beloukas, AParaskevis, DHaida, CSypsa, VHatzakis, AengEvaluation Study2009/05/08 09:00J Clin Microbiol. 2009 Jul;47(7):2194-9. doi: 10.1128/JCM.01264-08. Epub 2009 May 6.</style></notes><custom2><style face="normal" font="default" size="100%">2708521</style></custom2><auth-address><style face="normal" font="default" size="100%">Department of Hygiene, Epidemiology and Medical Statistics, Medical School, University of Athens, Athens, Greece.</style></auth-address></record></records></xml>