<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Katsoulidou, A</style></author><author><style face="normal" font="default" size="100%">Sypsa, V</style></author><author><style face="normal" font="default" size="100%">Tassopoulos, N. C.</style></author><author><style face="normal" font="default" size="100%">Boletis, J.</style></author><author><style face="normal" font="default" size="100%">Karafoulidou, A.</style></author><author><style face="normal" font="default" size="100%">Ketikoglou, I.</style></author><author><style face="normal" font="default" size="100%">Tsantoulas, D.</style></author><author><style face="normal" font="default" size="100%">Vafiadi, I.</style></author><author><style face="normal" font="default" size="100%">Hatzis, G.</style></author><author><style face="normal" font="default" size="100%">Skoutelis, A</style></author><author><style face="normal" font="default" size="100%">Akriviadis, E.</style></author><author><style face="normal" font="default" size="100%">Vasiliadis, T.</style></author><author><style face="normal" font="default" size="100%">Kitis, G.</style></author><author><style face="normal" font="default" size="100%">Magiorkinis, G</style></author><author><style face="normal" font="default" size="100%">Hatzakis, A</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Molecular epidemiology of hepatitis C virus (HCV) in Greece: temporal trends in HCV genotype-specific incidence and molecular characterization of genotype 4 isolates</style></title><secondary-title><style face="normal" font="default" size="100%">J Viral HepatJ Viral HepatJ Viral Hepat</style></secondary-title><alt-title><style face="normal" font="default" size="100%">Journal of viral hepatitis</style></alt-title><short-title><style face="normal" font="default" size="100%">Journal of viral hepatitisJournal of viral hepatitis</style></short-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">Adolescent</style></keyword><keyword><style  face="normal" font="default" size="100%">Adult</style></keyword><keyword><style  face="normal" font="default" size="100%">Aged</style></keyword><keyword><style  face="normal" font="default" size="100%">Aged, 80 and over</style></keyword><keyword><style  face="normal" font="default" size="100%">Base Sequence</style></keyword><keyword><style  face="normal" font="default" size="100%">Child</style></keyword><keyword><style  face="normal" font="default" size="100%">Cluster analysis</style></keyword><keyword><style  face="normal" font="default" size="100%">Female</style></keyword><keyword><style  face="normal" font="default" size="100%">Genotype</style></keyword><keyword><style  face="normal" font="default" size="100%">Greece/epidemiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Hepacivirus/*genetics/isolation &amp; purification</style></keyword><keyword><style  face="normal" font="default" size="100%">Hepatitis C/*epidemiology</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Incidence</style></keyword><keyword><style  face="normal" font="default" size="100%">Male</style></keyword><keyword><style  face="normal" font="default" size="100%">Middle Aged</style></keyword><keyword><style  face="normal" font="default" size="100%">Phylogeny</style></keyword><keyword><style  face="normal" font="default" size="100%">polymerase chain reaction</style></keyword><keyword><style  face="normal" font="default" size="100%">Reverse Transcriptase Polymerase Chain Reaction</style></keyword><keyword><style  face="normal" font="default" size="100%">RNA, Viral/chemistry/genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Sequence Analysis, DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Viral Nonstructural Proteins/chemistry/genetics</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2006</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jan</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">1</style></number><volume><style face="normal" font="default" size="100%">13</style></volume><pages><style face="normal" font="default" size="100%">19-27</style></pages><isbn><style face="normal" font="default" size="100%">1352-0504 (Print)1352-0504 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">This study aimed to estimate the overall HCV genotype distribution and to reconstruct the HCV genotype-specific incidence in Greece during the recent decades. It also focused at the identification of genotype 4 subtype variability in Greek isolates. A total of 1686 chronically infected HCV patients with detectable serum HCV RNA by RT-PCR, belonging to different risk groups were studied. Amplified products from the 5'-noncoding region were typed using a commercially available assay based on the reverse hybridization principle. The HCV genotype-specific incidence was estimated using a previously described back calculation method. HCV genotype 1 was the most prevalent (46.9%) followed by genotype 3 (28.1%), 4 (13.2%), 2 (6.9%) and 5 (0.4%). A high prevalence of genotype 1 (66.3%) in haemophilia patients was recorded whereas HCV genotype 3 was found mainly among patients infected by I.V. drug use (58.2%). Data on the temporal patterns of HCV genotype-specific incidence in Greece revealed a moderate increase (1.3-1.6 times) for genotypes 1 and 4, and a decrease (1.5 times) for genotype 2 from 1970 to 1990, whereas there was a sharp (13-fold) increase for genotype 3. The molecular characterization of 41 genotype 4 HCV isolates belonging to various risk groups revealed that, subtype 4a was the most frequently detected (78%). Phylogenetic comparison of the Greek 4a isolates with all HCV-4a isolates reported worldwide so far revealed a topology which does not discriminate Greek isolates from the others. HCV-4 does not represent a recent introduction in Greece.</style></abstract><accession-num><style face="normal" font="default" size="100%">16364078</style></accession-num><notes><style face="normal" font="default" size="100%">Katsoulidou, ASypsa, VTassopoulos, N CBoletis, JKarafoulidou, AKetikoglou, ITsantoulas, DVafiadi, IHatzis, GSkoutelis, AAkriviadis, EVasiliadis, TKitis, GMagiorkinis, GHatzakis, AengEngland2005/12/21 09:00J Viral Hepat. 2006 Jan;13(1):19-27. doi: 10.1111/j.1365-2893.2005.00649.x.</style></notes><auth-address><style face="normal" font="default" size="100%">Department of Hygiene and Epidemiology, Athens University Medical School, Athens, Greece.</style></auth-address></record></records></xml>