<?xml version="1.0" encoding="UTF-8"?><xml><records><record><source-app name="Biblio" version="7.x">Drupal-Biblio</source-app><ref-type>17</ref-type><contributors><authors><author><style face="normal" font="default" size="100%">Anastassopoulou, C. G.</style></author><author><style face="normal" font="default" size="100%">Paraskevis, D</style></author><author><style face="normal" font="default" size="100%">Sypsa, V. A.</style></author><author><style face="normal" font="default" size="100%">Chryssou, S. E.</style></author><author><style face="normal" font="default" size="100%">Antoniadou, A</style></author><author><style face="normal" font="default" size="100%">Giamarelou, H.</style></author><author><style face="normal" font="default" size="100%">Hatzakis, A</style></author></authors></contributors><titles><title><style face="normal" font="default" size="100%">Genetic evolution of human immunodeficiency virus type 1 in two spouses responding successfully to highly active antiretroviral therapy</style></title><secondary-title><style face="normal" font="default" size="100%">AIDS Res Hum RetrovirusesAIDS Res Hum RetrovirusesAIDS Res Hum Retroviruses</style></secondary-title><alt-title><style face="normal" font="default" size="100%">AIDS research and human retroviruses</style></alt-title><short-title><style face="normal" font="default" size="100%">AIDS research and human retrovirusesAIDS research and human retroviruses</style></short-title></titles><keywords><keyword><style  face="normal" font="default" size="100%">*Antiretroviral Therapy, Highly Active</style></keyword><keyword><style  face="normal" font="default" size="100%">*Evolution, Molecular</style></keyword><keyword><style  face="normal" font="default" size="100%">*Spouses</style></keyword><keyword><style  face="normal" font="default" size="100%">DNA, Viral/analysis/genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Female</style></keyword><keyword><style  face="normal" font="default" size="100%">Genes, env</style></keyword><keyword><style  face="normal" font="default" size="100%">HIV Infections/*drug therapy/virology</style></keyword><keyword><style  face="normal" font="default" size="100%">HIV-1/*genetics</style></keyword><keyword><style  face="normal" font="default" size="100%">Humans</style></keyword><keyword><style  face="normal" font="default" size="100%">Likelihood Functions</style></keyword><keyword><style  face="normal" font="default" size="100%">Male</style></keyword><keyword><style  face="normal" font="default" size="100%">Molecular Sequence Data</style></keyword><keyword><style  face="normal" font="default" size="100%">Phylogeny</style></keyword><keyword><style  face="normal" font="default" size="100%">Selection, Genetic</style></keyword><keyword><style  face="normal" font="default" size="100%">Sequence Analysis, DNA</style></keyword><keyword><style  face="normal" font="default" size="100%">Treatment Outcome</style></keyword></keywords><dates><year><style  face="normal" font="default" size="100%">2003</style></year><pub-dates><date><style  face="normal" font="default" size="100%">Jan 1</style></date></pub-dates></dates><number><style face="normal" font="default" size="100%">1</style></number><volume><style face="normal" font="default" size="100%">19</style></volume><pages><style face="normal" font="default" size="100%">65-71</style></pages><isbn><style face="normal" font="default" size="100%">0889-2229 (Print)0889-2229 (Linking)</style></isbn><language><style face="normal" font="default" size="100%">eng</style></language><abstract><style face="normal" font="default" size="100%">The current case study provided an unusual setting to track the evolution of HIV-1 envelope gene over a maximum period of 6 years in two asymptomatic spouses undergoing suppressive highly active antiretroviral therapy. For this purpose, proviral DNA samples taken from uncultured peripheral blood mononuclear cells and spanning the C2-V5 regions of env were analyzed at three sampling points per subject. Two distinct topological patterns were observed in the phylogenetic reconstructions of the genetically linked sequences of the couple: an intermingled pattern and a sequentially shifting pattern in the virus populations of the male index case and his spouse, respectively. Application of three evolutionary models for the amino acid-encoded sites, using the maximum likelihood approach, indicated the operation of positive selection in the region only at the second time point in the woman, before receiving therapy. These findings reinforce the evidence of a crucial role for host-selective constraints on HIV-1 env evolution in vivo.</style></abstract><accession-num><style face="normal" font="default" size="100%">12596725</style></accession-num><notes><style face="normal" font="default" size="100%">Anastassopoulou, Cleo GParaskevis, DimitriosSypsa, Vassiliki-AnastasiaChryssou, Stella-EugenieAntoniadou, AnastasiaGiamarelou, HelenHatzakis, AngelosengResearch Support, Non-U.S. Gov't2003/02/25 04:00AIDS Res Hum Retroviruses. 2003 Jan 1;19(1):65-71. doi: 10.1089/08892220360473989.</style></notes><auth-address><style face="normal" font="default" size="100%">National Retrovirus Reference Center, Department of Hygiene and Epidemiology, Athens University Medical School, Greece.</style></auth-address></record></records></xml>