Publications

2019
Misailidi N, Athanaselis S, Nikolaou P, Katselou M, Dotsikas Y, Spiliopoulou C, Papoutsis I. A GC–MS method for the determination of furanylfentanyl and ocfentanil in whole blood with full validation. Forensic Toxicology [Internet]. 2019;37:238-244. Website
Rmandić M, Dotsikas Y, Malenović A. Identification of the factors affecting the consistency of DBS formation via experimental design and image processing methodology. Microchemical Journal [Internet]. 2019;145:1003-1010. Website
2018
Alexovič M, Dotsikas Y, Bober P, Sabo J. Achievements in robotic automation of solvent extraction and related approaches for bioanalysis of pharmaceuticals. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences [Internet]. 2018;1092:402-421. Website
Schulpis KH, Thodi G, Iakovou K, Dotsikas Y, Molou E, Loukas YL. Identification of five mutations in a patient with galactose metabolic disorders. Journal of Pediatric Endocrinology and Metabolism [Internet]. 2018;31:221-222. Website
Iliou K, Malenović A, Loukas YL, Dotsikas Y. Analysis of potential genotoxic impurities in rabeprazole active pharmaceutical ingredient via Liquid Chromatography-tandem Mass Spectrometry, following quality-by-design principles for method development. Journal of Pharmaceutical and Biomedical Analysis [Internet]. 2018;149:410-418. Website
2017
Grigori K, Loukas YL, Malenović A, Samara V, Kalaskani A, Dimovasili E, Kalovidouri M, Dotsikas Y. Chemometrically assisted development and validation of LC–MS/MS method for the analysis of potential genotoxic impurities in meropenem active pharmaceutical ingredient. Journal of Pharmaceutical and Biomedical Analysis [Internet]. 2017;145:307-314. Website
Schulpis KH, Thodi G, Iakovou K, Chatzidaki M, Dotsikas Y, Molou E, Triantafylli O, Loukas YL. Clinical evaluation and mutational analysis of GALK and GALE genes in patients with galactosemia in Greece: One novel mutation and two rare cases. Journal of Pediatric Endocrinology and Metabolism [Internet]. 2017;30:775-779. Website
Vlachou M, Papamichael M, Siamidi A, Fragouli I, Afroudakis PA, Kompogennitaki R, Dotsikas Y. Comparative in vitro controlled release studies on the chronobiotic hormone melatonin from cyclodextrins-containing matrices and cyclodextrin: Melatonin complexes. International Journal of Molecular Sciences [Internet]. 2017;18. Website
Schulpis KH, Thodi G, Iakovou K, Chatzidaki M, Dotsikas Y, Molou E, Triantafylli O, Loukas YL. Mutational analysis of GALT gene in Greek patients with galactosaemia: identification of two novel mutations and clinical evaluation. Scandinavian Journal of Clinical and Laboratory Investigation [Internet]. 2017;77:423-427. Website
Schulpis KH, Thodi G, Chatzidaki M, Iakovou K, Molou E, Dotsikas Y, Loukas YL. Rare cases of galactose metabolic disorders: Identification of more than two mutations per patient. Journal of Pediatric Endocrinology and Metabolism [Internet]. 2017;30:1119-1120. Website
Koukoula M, Dotsikas Y, Molou E, Schulpis KH, Thodi G, Chatzidaki M, Triantafylli O, Loukas YL. Study of the effect of CYP2C19 polymorphisms on omeprazole pharmacokinetics by utilizing validated LC–MS/MS and Real Time-PCR methods. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences [Internet]. 2017;1047:173-179. Website
2016
Thodi G, Schulpis KH, Dotsikas Y, Pavlides C, Molou E, Chatzidaki M, Triantafylli O, Loukas YL. Hawkinsinuria in two unrelated Greek newborns: Identification of a novel variant, biochemical findings and treatment. Journal of Pediatric Endocrinology and Metabolism [Internet]. 2016;29:15-20. Website
Thodi G, Schulpis KH, Hatzidaki M, Molou E, Triantafylli O, Dotsikas Y, Loukas YL. Partial biotinidase deficiency: Identification of a single novel mutation (p.H314R) in a Greek newborn. Journal of Pediatric Endocrinology and Metabolism [Internet]. 2016;29:389-390. Website
Foivas A, Malenović A, Kostić N, Božić M, Knežević M, Loukas YL, Dotsikas Y. Quantitation of brinzolamide in dried blood spots by a novel LC-QTOF-MS/MS method. Journal of Pharmaceutical and Biomedical Analysis [Internet]. 2016;119:84-90. Website
2015
Kritikos N, Vallianatou T, Dotsikas Y, Tsantili-Kakoulidou A. Chemometrics-chem(O)informatics as a tool in quantitative structure – Property and structure – Activity relationships, qspr & qsar. Pharmakeftiki [Internet]. 2015;27:73-100. Website
Loukas YL, Thodi G, Molou E, Georgiou V, Dotsikas Y, Schulpis KH. Clinical diagnostic Next-Generation sequencing: The case of CFTR carrier screening. Scandinavian Journal of Clinical and Laboratory Investigation [Internet]. 2015;75(5):374 - 381. Website
Molou E, Schulpis KH, Birbilis C, Thodi G, Georgiou V, Dotsikas Y, Loukas YL. Early screening of FTO and MC4R variants in newborns of Greek origin. Journal of Pediatric Endocrinology and Metabolism [Internet]. 2015;28(5-6):619 - 622. Website
Kostić N, Dotsikas Y, Jović N, Stevanović G, Malenović A, Medenica M. Quantitation of pregabalin in dried blood spots and dried plasma spots by validated LC-MS/MS methods. Journal of Pharmaceutical and Biomedical Analysis [Internet]. 2015;109:79 - 84. Website
Kritikos N, Loukas YL, Dotsikas Y. Quantitative structure-chemiluminescence intensity relationships of 4-substituted phenols acting as luminol signal enhancers. Chemometrics and Intelligent Laboratory Systems [Internet]. 2015;146:478 - 484. Website
Kritikos N, Tsantili-Kakoulidou A, Loukas YL, Dotsikas Y. Liquid chromatography coupled to quadrupole-time of flight tandem mass spectrometry based quantitative structure-retention relationships of amino acid analogues derivatized via n-propyl chloroformate mediated reaction. Journal of Chromatography A [Internet]. 2015;1403:70-80. Publisher's Version
2014
Kostić N, Dotsikas Y, Malenović A. Critical review on the analytical methods for the determination of zwitterionic antiepileptic drugs-Vigabatrin, Pregabalin, and Gabapentin - In bulk and formulations. Instrumentation Science and Technology [Internet]. 2014;42(4):486 - 512. Website
Molou E, Schulpis KH, Thodi G, Georgiou V, Dotsikas Y, Papadopoulos K, Biti S, Loukas YL. Glucose-6-Phosphate Dehydrogenase (G6PD) deficiency in Greek newborns: The Mediterranean C563T mutation screening. Scandinavian Journal of Clinical and Laboratory Investigation [Internet]. 2014;74(3):259 - 263. Website
Labrineas C, Varverakis E, Pistos C, Dotsikas I, Gikas E, Papoutsis I, Spiliopoulou C, Athanaselis S. Overcoming insufficient fragmentation in single quadruple MS by combining HPLC-UV/Vis with LC/MS: The case of risperidone in terms of the identification point system. Pharmakeftiki [Internet]. 2014;26(2):66 - 76. Website
Kostić N, Dotsikas Y, Jović N, Stevanović G, Malenović A, Medenica M. Vigabatrin in dried plasma spots: Validation of a novel LC-MS/MS method and application to clinical practice. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences [Internet]. 2014;962:102 - 108. Website
2013
Jančic Stojanovic B, Dotsikas Y. Chemometrics in Chromatography. Chromatographia [Internet]. 2013;76(5-6):207 - 209. Website
Kostić NDS, Dotsikas Y, Malenović ADS, Medenica M. Effects of derivatization reagents consisting of n-alkyl chloroformate/n-alcohol combinations in LC-ESI-MS/MS analysis of zwitterionic antiepileptic drugs. Talanta [Internet]. 2013;116:91 - 99. Website
Kostic̈ NSS, Dotsikas Y, Malenovic̈ ASS, Stojanovic̈ BJ, Rakic̈ T, Ivanovic̈ D, Medenica M. Stepwise optimization approach for improving LC-MS/MS analysis of zwitterionic antiepileptic drugs with implementation of experimental design. Journal of Mass Spectrometry [Internet]. 2013;48(7):875 - 884. Website
Thodi G, Schulpis KH, Molou E, Georgiou V, Loukas YL, Dotsikas Y, Papadopoulos K, Biti S. High incidence of partial biotinidase deficiency cases in newborns of Greek origin. Gene [Internet]. 2013;524(2):361 - 362. Website
2012
Thodi G, Georgiou V, Molou E, Loukas YL, Dotsikas Y, Biti S, Papadopoulos K, Doulgerakis E. Characterization of the molecular spectrum of Medium-Chain Acyl-CoA Dehydrogenase Deficiency in a Greek newborns cohort: Identification of a novel variant. Clinical Biochemistry [Internet]. 2012;45(15):1167 - 1172. Website
Marquardt G, Currier R, McHugh DMS, Gavrilov D, Magera MJ, Matern D, Oglesbee D, Raymond K, Rinaldo P, Smith EH, et al. Enhanced interpretation of newborn screening results without analyte cutoff values. Genetics in Medicine [Internet]. 2012;14(7):648 - 655. Website
Dotsikas Y, Loukas YL. Improved performance of antigen-HRP conjugate-based immunoassays after the addition of anti-HRP antibody and application of a liposomal chemiluminescence marker. Analytical Sciences [Internet]. 2012;28(8):753 - 757. Website
Maltezou HC, Drakoulis N, Siahanidou T, Karalis V, Zervaki E, Dotsikas Y, Loukas YL, Theodoridou M. Safety and pharmacokinetics of oseltamivir for prophylaxis of neonates exposed to influenza H1N1. Pediatric Infectious Disease Journal [Internet]. 2012;31(5):527 - 529. Website
2011
Kolocouri F, Dotsikas Y, Apostolou C, Kousoulos C, Soumelas G-S, Loukas YL. Advantages of automation in plasma sample preparation prior to HPLC/MS/MS quantification: Application to the determination of cilazapril and cilazaprilat in a bioequivalence study. Journal of AOAC International [Internet]. 2011;94(3):758 - 764. Website
McHugh DMS, Cameron CA, Abdenur JE, Abdulrahman M, Adair O, Al Nuaimi SA, Åhlman H, Allen JJ, Antonozzi I, Archer S, et al. Clinical validation of cutoff target ranges in newborn screening of metabolic disorders by tandem mass spectrometry: A worldwide collaborative project. Genetics in Medicine [Internet]. 2011;13(3):230 - 254. Website
Dotsikas Y, Markopoulou CK, Koundourellis JE, Loukas YL. Validation of a novel LC-MS/MS method for the quantitation of colistin A and B in human plasma. Journal of Separation Science [Internet]. 2011;34(1):37 - 45. Website
Maskovic M, Dotsikas Y, Malenovic A, Jancic-Stojanovic B, Ivanovic D, Medenica M. Validation of an oil-in-water microemulsion liquid chromatography method for analysis of perindopril tert-butylamine and its impurities. Journal of AOAC International [Internet]. 2011;94(3):723 - 734. Website
Malenović A, Dotsikas Y, Mašković M, Jančić-Stojanović B, Ivanović D, Medenica M. Desirability-based optimization and its sensitivity analysis for the perindopril and its impurities analysis in a microemulsion LC system. Microchemical Journal [Internet]. 2011;99(2):454 - 460. Website
Thodi G, Molou E, Georgiou V, Loukas YL, Dotsikas Y, Biti S, Papadopoulos K, Konstantinou D, Antoniadi M, Doulgerakis E. Mutational analysis for biotinidase deficiency of a Greek patients cohort ascertained through expanded newborn screening. Journal of Human Genetics [Internet]. 2011;56(12):861 - 865. Website
2010
Kolocouri F, Dotsikas Y, Loukas YL. Dried plasma spots as an alternative sample collection technique for the quantitative LC-MS/MS determination of gabapentin. Analytical and Bioanalytical Chemistry [Internet]. 2010;398(3):1339 - 1347. Website
Loukas YL, Soumelas G-S, Dotsikas Y, Georgiou V, Molou E, Thodi G, Boutsini M, Biti S, Papadopoulos K. Expanded newborn screening in Greece: 30 months of experience. Journal of Inherited Metabolic Disease [Internet]. 2010;33(SUPPL. 3):S341 - S348. Website
Kousoulos C, Dotsikas Y, Loukas YL. Optimizing the generalization ability of artificial neural networks in ELISA protocols by employing different topologies and GENETIC operators. In: Reviews in Pharmaceutical and Biomedical Analysis. ; 2010. pp. 20 - 29. Website
Dotsikas Y, Apostolou C, Soumelas S, Kolocouri F, Ziaka A, Kousoulos C, Loukas YL. Validation of a novel, fully automated high throughput high-performance liquid chromatographic/tandem mass spectrometric method for quantification of pantoprazole in human plasma. Journal of AOAC International [Internet]. 2010;93(4):1129 - 1136. Website
2008
Apostolou C, Kousoulos C, Dotsikas Y, Soumelas G-S, Kolocouri F, Ziaka A, Loukas YL. An improved and fully validated LC-MS/MS method for the simultaneous quantification of simvastatin and simvastatin acid in human plasma. Journal of Pharmaceutical and Biomedical Analysis [Internet]. 2008;46(4):771 - 779. Website
Kousoulos C, Tsatsou G, Dotsikas Y, Apostolou C, Loukas YL. Validation of a fully automated high throughput liquid chromatographic/tandem mass spectrometric method for roxithromycin quantification in human plasma. Application to a bioequivalence study. Biomedical Chromatography [Internet]. 2008;22(5):494 - 501. Website
Apostolou C, Kousoulos C, Dotsikas Y, Loukas YL. Comparison of hydrophilic interaction and reversed-phase liquid chromatography coupled with tandem mass spectrometric detection for the determination of three pharmaceuticals in human plasma. Biomedical Chromatography [Internet]. 2008;22(12):1393 - 1402. Website
Apostolou C, Dotsikas Y, Kousoulos C, Loukas YL. Development and validation of an improved high-throughput method for the determination of anastrozole in human plasma by LC-MS/MS and atmospheric pressure chemical ionization. Journal of Pharmaceutical and Biomedical Analysis [Internet]. 2008;48(3):853 - 859. Website
2007
Apostolou C, Dotsikas Y, Kousoulos C, Tsatsou G, Colocouri F, Soumelas G-S, Loukas YL. Application of a semi-automated 96-well format solid-phase extraction, column-switching, fluorescence detection protocol for the determination of alendronate in human urine samples obtained from a bioequivalence study. Journal of Pharmaceutical and Biomedical Analysis [Internet]. 2007;43(3):1151 - 1155. Website
Dotsikas Y, Loukas YL. Effect of the luminol signal enhancer selection on the curve parameters of an immunoassay and the chemiluminescence intensity and kinetics. Talanta [Internet]. 2007;71(2):906 - 910. Website
Dotsikas Y, Apostolou C, Kousoulos C, Tsatsou G, Loukas YL. An improved high-throughput liquid chromatographic/tandem mass spectrometric method for terbinafine quantification in human plasma, using automated liquid-liquid extraction based on 96-well format plates. Biomedical Chromatography [Internet]. 2007;21(2):201 - 208. Website
Apostolou C, Dotsikas Y, Kousoulos C, Loukas YL. Quantitative determination of donepezil in human plasma by liquid chromatography/tandem mass spectrometry employing an automated liquid-liquid extraction based on 96-well format plates. Application to a bioequivalence study. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences [Internet]. 2007;848(2):239 - 244. Website
Kolocouri F, Dotsikas Y, Apostolou C, Kousoulos C, Loukas YL. Simultaneous determination of losartan, EXP-3174 and hydrochlorothiazide in plasma via fully automated 96-well-format-based solid-phase extraction and liquid chromatography-negative electrospray tandem mass spectrometry. Analytical and Bioanalytical Chemistry [Internet]. 2007;387(2):593 - 601. Website
Kousoulos C, Dotsikas Y, Loukas YL. Turbulent flow and ternary column-switching on-line clean-up system for high-throughput quantification of risperidone and its main metabolite in plasma by LC-MS/MS. Application to a bioequivalence study. Talanta [Internet]. 2007;72(2):360 - 367. Website
2006
Dotsikas Y, Kousoulos C, Tsatsou G, Loukas YL. Development and validation of a rapid 96-well format based liquid-liquid extraction and liquid chromatography-tandem mass spectrometry analysis method for ondansetron in human plasma. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences [Internet]. 2006;836(1-2):79 - 82. Website
Kousoulos C, Tsatsou G, Apostolou C, Dotsikas Y, Loukas YL. Development of a high-throughput method for the determination of itraconazole and its hydroxy metabolite in human plasma, employing automated liquid-liquid extraction based on 96-well format plates and LC/MS/MS. Analytical and Bioanalytical Chemistry [Internet]. 2006;384(1):199 - 207. Website
2005
Dotsikas Y, Loukas YL. Application of avidin-biotin technology for the characterization of a model hapten-protein conjugate. Journal of Immunoassay and Immunochemistry [Internet]. 2005;26(4):285 - 293. Website
Kousoulos C, Tsatsou G, Dotsikas Y, Loukas YL. Development of a rapid liquid chromatography tandem mass spectrometry method for the determination of lisinopril, applicable for a bioequivalence study, employing a 96-well format solid phase extraction protocol. Analytica Chimica Acta [Internet]. 2005;551(1-2):177 - 183. Website
Dotsikas Y, Kousoulos C, Tsatsou G, Loukas YL. Development of a rapid method for the determination of glimepiride in human plasma using liquid-liquid extraction based on 96-well format micro-tubes and liquid chromatography/tandem mass spectrometry. Rapid Communications in Mass Spectrometry [Internet]. 2005;19(14):2055 - 2061. Website
Zoidis G, Papanastasiou I, Dotsikas I, Sandoval A, Dos Santos RG, Papadopoulou-Daifoti Z, Vamvakides A, Kolocouris N, Felix R. The novel GABA adamantane derivative (AdGABA): Design, synthesis, and activity relationship with gabapentin. Bioorganic and Medicinal Chemistry [Internet]. 2005;13(8):2791 - 2798. Website
2004
Dotsikas Y, Loukas YL. Employment of 4-(1-imidazolyl)phenol as a luminol signal enhancer in a competitive-type chemiluminescence immunoassay and its comparison with the conventional antigen-horseradish peroxidase conjugate-based assay. Analytica Chimica Acta [Internet]. 2004;509(1):103 - 109. Website
Sarikaki V, Rallis M, Tanojo H, Panteri I, Dotsikas Y, Loukas YL, Papaioannou G, Demetzos C, Weber S, Moini H, et al. In vitro percutaneous absorption of pine bark extract (pycnogenol) in human skin. Journal of Toxicology - Cutaneous and Ocular Toxicology [Internet]. 2004;23(3):149 - 158. Website
2003
Dotsikas Y, Loukas YL. Efficient determination and evaluation of model cyclodextrin complex binding constants by electrospray mass spectrometry. Journal of the American Society for Mass Spectrometry [Internet]. 2003;14(10):1123 - 1129. Website
2002
Dotsikas Y, L.Loukas Y, Siafaka I. Determination of umbilical cord and maternal plasma concentrations of fentanyl by using novel spectrophotometric and chemiluminescence enzyme immunoassays. Analytica Chimica Acta [Internet]. 2002;459(2):177 - 185. Website
Dotsikas Y, Loukas YL. Inclusional complex study between 6-p-toluidinylnaphthalene-2-sulfonate and 2-hydroxypropyl-beta-cyclodextrin. Journal of Biochemical and Biophysical Methods [Internet]. 2002;52(2):121 - 134. Website
Dotsikas Y, Loukas YL. Kinetic degradation study of insulin complexed with methyl-beta cyclodextrin. Confirmation of complexation with electrospray mass spectrometry and 1H NMR. Journal of Pharmaceutical and Biomedical Analysis [Internet]. 2002;29(3):487 - 494. Website
2000
Dotsikas Y, Kontopanou E, Allagiannis C, Loukas YL. Interaction of 6-p-toluidinylnaphthalene-2-sulphonate with β-cyclodextrin. Journal of Pharmaceutical and Biomedical Analysis [Internet]. 2000;23(6):997 - 1003. Website