Abstract:

The muscle LIM protein (MLP) and cofilin 2 (CFL2) are important regulators of striated myocyte function. Mutations in the corresponding genes have been directly associated with severe human cardiac and skeletal myopathies, and aberrant expression patterns have often been observed in affected muscles. Herein, we have investigated whether MLP and CFL2 are involved in common molecular mechanisms, which would promote our understanding of disease pathogenesis. We have shown for the first time, using a range of biochemical and immunohistochemical methods, that MLP binds directly to CFL2 in human cardiac and skeletal muscles. The interaction involves the inter-LIM domain, amino acids 94 to 105, of MLP and the amino-terminal domain, amino acids 1 to 105, of CFL2, which includes part of the actin depolymerization domain. The MLP/CFL2 complex is stronger in moderately acidic (pH 6.8) environments and upon CFL2 phosphorylation, while it is independent of Ca(2+) levels. This interaction has direct implications in actin cytoskeleton dynamics in regulating CFL2-dependent F-actin depolymerization, with maximal depolymerization enhancement at an MLP/CFL2 molecular ratio of 2:1. Deregulation of this interaction by intracellular pH variations, CFL2 phosphorylation, MLP or CFL2 gene mutations, or expression changes, as observed in a range of cardiac and skeletal myopathies, could impair F-actin depolymerization, leading to sarcomere dysfunction and disease.

Notes:

Papalouka, VasilikiArvanitis, Demetrios AVafiadaki, ElizabethMavroidis, ManolisPapadodima, Stavroula ASpiliopoulou, Chara AKremastinos, Dimitrios TKranias, Evangelia GSanoudou, DespinaengHL26057/HL/NHLBI NIH HHS/R37 HL026057/HL/NHLBI NIH HHS/HL77101/HL/NHLBI NIH HHS/R01 HL026057/HL/NHLBI NIH HHS/HL64018/HL/NHLBI NIH HHS/R01 HL064018/HL/NHLBI NIH HHS/P50 HL077101/HL/NHLBI NIH HHS/Research Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov't2009/09/16 06:00Mol Cell Biol. 2009 Nov;29(22):6046-58. doi: 10.1128/MCB.00654-09. Epub 2009 Sep 14.