Abstract:

We tested 27 microsatellite loci for cross-species amplification in the lacertids Podarcis gaigeae and Podarcis hispanica. We detected 11 and 15 polymorphic loci in the former and the latter species, respectively. In a larger sample of individuals from a single population of each species, the number of alleles ranged from five to 23 in 10 of the polymorphic loci in P. gaigeae, and between four and 13 in nine of polymorphic loci in P. hispanica. Two locus deviated from Hardy-Weinberg equilibrium in P. hispanica. Between 11 and 16 of the 27 loci also amplified successfully in three other Podarcis species. © 2008 The Authors.

Notes:

Cited By (since 1996): 4Export Date: 20 August 2012Source: Scopusdoi: 10.1111/j.1755-0998.2008.02299.xLanguage of Original Document: EnglishCorrespondence Address: Runemark, A.; Department of Animal Ecology, Ecology Building, Lund University, SE-22362 Lund, Sweden; email: anna.runemark@zooekol.lu.seReferences: Arnold, N.E., Ovenden, D.W., (2002) A Field Guide to the Reptiles and Amphibians of Britain and Europe, , 2nd edn. Harper Collins Publishers, London; Boudjemadi, K., Martin, O., Simon, J.C., Estoup, A., Development and cross-species comparison of microsatellite markers in two lizard species, Lacerta vivipara and Podarcis muralis (1999) Molecular Ecology, 8, pp. 518-520; Goudet, J., (2001), http://www.unil.ch/popgen/softwares/fstat.htm, Version 2.9.3. Available from URLNembrini, M., Oppliger, A., Characterization of microsatellite loci in the wall lizard Podarcis muralis (Sauria: Lacertidae) (2003) Molecular Ecology Notes, 3, pp. 123-124; Pinho, C., Sequeira, F., Godinho, R., Harris, D.J., Ferrand, N., Isolation and characterization of nine microsatellite loci in Podarcis bocagei (Squamata: Lacertidae) (2004) Molecular Ecology Notes, 4, pp. 286-288; Poulakakis, N., Goulielmos, G., Antoniou, A., Zouros, E., Mylonas, M., Isolation and characterization of polymorphic microsatellite markers in the wall lizard Podarcis erhardii (Squamata: Lacertidae) (2005) Molecular Ecology Notes, 5, pp. 549-551; Poulakakis, N., Lymberakis, P., Valakos, E., Zouros, E., Mylonas, M., Phylogenetic relationships and biogeography of Podarcis species from the Balkan Peninsula, by Bayesian and maximum likelihood analyses of mitochondrial DNA sequences (2005) Molecular Phylogenetics and Evolution, 37, pp. 845-857; Sambrook, J., Fritsch, E.F., Maniatis, T., (1989) Molecular Cloning: A Laboratory Manual, , 2nd edn. Cold Spring Harbor Laboratory Press, New York; Schneider, S., Roessli, D., Excoffier, L., (2000), http://lgb.unige.ch/arlequin/software/, Version 2.0. Available from URLUR - http://www.scopus.com/inward/record.url?eid=2-s2.0-55149104199&partnerID...