Abstract:

The rate of oxygen consumption is a vital marker indicating cellular function during lifetime under normal or metabolically challenged conditions. It is used broadly to study mitochondrial function (Artal-Sanz and Tavernarakis, 2009; Palikaras et al., 2015; Ryu et al., 2016) or investigate factors mediating the switch from oxidative phosphorylation to aerobic glycolysis (Chen et al., 2015; Vander Heiden et al., 2009). In this protocol, we describe a method for the determination of oxygen consumption rates in the nematode Caenorhabditis elegans.

Notes:

Palikaras, KonstantinosTavernarakis, Nektarioseng233358/European Research Council/InternationalBio Protoc. 2016 Dec 5;6(23). doi: 10.21769/BioProtoc.2049.