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Comparison of three current viral load assays for the quantitation of human immunodeficiency virus type 1 RNA in plasma

Citation:

Katsoulidou A, Papachristou E, Petrodaskalaki M, Sypsa V, Anastassopoulou CG, Gargalianos P, Karafoulidou A, Lazanas M, Kordossis T, Andoniadou A, et al. Comparison of three current viral load assays for the quantitation of human immunodeficiency virus type 1 RNA in plasma. J Virol MethodsJ Virol MethodsJ Virol Methods. 2004;121:93-9.

Abstract:

The LCx HIV RNA quantitative assay (Abbott Laboratories, Delkenheim, Germany) was compared with the Versant HIV-1 RNA 3.0 (bDNA) assay (Bayer, Tarrytown, NY) and the COBAS Amplicor HIV-1 Monitor v1.5 test (Roche Diagnostics, Branchburg, NJ), using plasma samples of various viral load levels from HIV-1-infected patients. Considering the lower limit of the linear range of 50 copies/ml of both assays, the detection range of the LCx was 127/151 (84.1%) versus the 131/151 (86.8%) of the bDNA 3.0 assay, while overall agreement between the two assays was 93.4% (141/151). LCx and bDNA 3.0 results were found to be strongly correlated (r = 0.96). The fitted regression line was described by the equation log10(LCx copies/ml) = 0.05 + 1.06 x log10(bDNA 3.0 copies/ml) with 95% CI for the estimated slope and intercept at 1.01, 1.12 and -0.16, 0.26, respectively. Similarly, the detection range of the LCx was 115/148 (77.7%) versus the 128/148 (86.5%) of the Monitor v1.5 test. A 91.2% concordance (135/148) was observed between these two assays at a cut-off of 50 copies/ml. LCx and Monitor v1.5 results were highly correlated (r = 0.96). The fitted regression line was described by the equation log10(LCx copies/ml) = 0.06 + 1.03 x log(10)(Monitor v1.5 copies/ml) with 95% CI for the estimated slope and intercept at 0.97, 1.09 and -0.16, 0.28, respectively.

Notes:

Katsoulidou, AntigoniPapachristou, EleniPetrodaskalaki, MariaSypsa, VanaAnastassopoulou, Cleo GGargalianos, PanagiotisKarafoulidou, AnastasiaLazanas, MariosKordossis, TheodorosAndoniadou, AnastasiaHatzakis, AngelosengComparative StudyNetherlands2004/09/08 05:00J Virol Methods. 2004 Oct;121(1):93-9. doi: 10.1016/j.jviromet.2004.06.007.