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Dimerization of Thermomonospora fusca beta-1,4-endoglucanase E2

Citation:

McGinnis K, Kroupis C, Wilson DB. Dimerization of Thermomonospora fusca beta-1,4-endoglucanase E2. Biochemistry [Internet]. 1993;32(32):8146 - 8150.

Abstract:

Unboiled Thermomonospora fusca endoglucanase E2 electrophoresed on SDS-polyacrylamide gels migrated in the range of 80-90 kDa, but when boiled it migrated in the 40-42-kDa range. Sedimentation equilibrium centrifugation as well as chemical cross-linking experiments confirmed that E2 is a dimer. The dimer was reversibly dissociated at low pH. The E2 dimer was stable up to 70 degrees C, but began to dissociate at this temperature after a 30-60-min incubation. A nondimerizing mutant was obtained using region-specific chemical mutagenesis. DNA sequencing of this mutant revealed a single base change that substituted Gly for Glu-263. Chemical modification of carboxylic acid residues in E2 disrupted the dimer interaction

Notes:

DA - 19930916 IS - 0006-2960 (Print) IS - 0006-2960 (Linking) LA - eng PT - Journal Article PT - Research Support, U.S. Gov't, Non-P.H.S RN - 0 (Cross-Linking Reagents) RN - 0 (Disulfides) RN - EC 3.2.1.4 (Cellulase) RN - T3C89M417N (Glutaral) SB - IM

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