Citation:
Krevvata MI, Afratis N, Spiliopoulou A, Malavaki CJ, Kolonitsiou F, Anastassiou E, Karamanos NK. A modified protocol for isolation and purity evaluation of a staphylococcal acidic polysaccharide by chromatography and capillary electrophoresis. Biomed ChromatogrBiomed Chromatogr. 2011;25:531-4.
Abstract:
The extracellular slime of Staphylococcus epidermidis contains, amongst various macromolecules, an acidic polysaccharide (PS) of a molecular mass of 20 kDa with significant antigenic and biological properties. The isolation procedure used so far includes multiple fractionations in anion-exchange chromatographic columns before its final purification by gel filtration chromatography. This protocol is laborious, time-consuming and includes the risk of unnecessary loss of PS quantities. Because of the significance of this PS, a modified protocol resulting in an easier and quicker isolation procedure was developed. Furthermore, identification, purity, charge density and molecular integrity of the isolated polysaccharide were evaluated by a reverse-polarity capillary electrophoresis method.Notes:
Krevvata, Maria IAfratis, NikosSpiliopoulou, AnastasiaMalavaki, Christina JKolonitsiou, FevroniaAnastassiou, EvangelosKaramanos, Nikos KengEngland2010/08/25Biomed Chromatogr. 2011 May;25(5):531-4. doi: 10.1002/bmc.1490. Epub 2010 Aug 23.
