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Brief Description of My Research Activities

• Design of novel drugs against various diseases (i.e hypertension and  inflammation)

To achieve this aim I use the following physical chemical methodologies:
a. Solid State Nuclear Magnetic Resonance in order to study the dynamic changes it causes the antihypertensive drug in the lipid bilayers. In addition,  the orientation of drugs in lipid bilayers  is  sought using deuterium NMR in collaboration with synthetic chemists who deuterate specific sites of drugs.  

b. High Resolution Nuclear Magnetic Resonance in duterated solvents or environments that simulate the active site of action.

c. Conformational Analysis Using NMR ROE data in combination with computational analysis and molecular modeling to complement the experimental results.

d. Differential Scanning Calorimetry to study the thermal changes that causes the presence of pharmaceutical compounds with model membranes.

e. X-ray diffraction where the topography of drugs is sought in lipid bilayers. The X-ray diffraction experiments are run in Graz of Austria under the guidance of Prof. P. Laggner.  

f. Raman Spectroscopy to study the interactions of bioactive compounds with lipid bilayers. In particular, the trans:gauche ratio is estimated at the different mesomorphic states of lipid bilayers.

g. 3D-QSAR studies. CoMFA and CoMSIA softwares are applied to reveal the stereoelectronic requirements for a drug to exert biological activity. 3D-QSAR studies is a valuable tool in the rational design.

h. Simulation of NMR spectra in liquid and solid state with the collaboration of theoretical physical chemists.

k. Docking studies of antihypertensive drugs with AT1 receptor.  

l. Searching for pharmacophore models in bioactive drug molecules.

• Authenticity aspects of beverages and foods

a. Study of geographical origin and adulteration of virgin olive oil by other cheaper oils using high resolution  ¹³C-NMR of olefinic region. In addition it is studied the adulteration of refined oil by refined hazelnut oil.

b. Study of authenticity of Cypriot beverage zivania with the combination of different physical chemical methods in collaboration with the University of Cyprus ( ¹Η-NMR and  Raman Spectroscopies, ICP, Gas Chromatography, Refractometry, Statistical Analysis to facilitate the analysis of  the output data etc).

VISITS AND SPECIALISATION AFTER PH.D STUDIES

1. Specialisation in the Computational Analysis and Molecular Modeling at Prof. Rod Hubbard’s laboratory (York University, Summer of 1992).
2. Invited visit  to Prof. Peter’s Laggner laboratory in order to perform small and wide angle x-ray diffraction experiments. The results from this visit are published in the Biochim. Biophys. Acta  (Summer of 1996).
3. Modern Experimental techniques using NMR spectrometer of AMX 500 MHz and use of modern software to process the experimental data (University of Connecticut, Summer of 1999).
4. Biological experiments related to the binding of novel AT1 antagonists at AT1 and AT2 receptors as well as cell cultures (University of Leeds, Summer of 2004).
5. Visits in the Synchrotron facilities of Trieste, Italy to perform x-ray expreriments (Summers 2007, 2009).
6. Visits of National Institute of Chemistry, Ljubluana, Slovenia (Summers of 2010 and 2012)
7. Visit of BMRZ in Frankfurt of Germany, (2011,2013,2014) running solid state NMR experiments using Bruker 600 and 850 MHz spectrometers. 

FUNDING-WORKSHOPS
1. Coordinator of a funded research project by the European Community entitled “Molecular Characterization of Virgin Olive Oil” (148.000 ECU).
2. Participation in the funded research project by the European Community entitled Development and Assessment of Methods for the Detection of Adulteration of Olive Oil with Hazelnut Oil GROWTH (MEDEO 2001).
3. Co-organization of a workshop with Maria Zervou held in Athens on November 22, 1996 about the “Use of Isotope Analyses Applications in Food and Beverage Authentication. Detection of Adulteration.” In this workshop six speakers from France, German and England informed the audience about the new techniques in the analyses and detection of adulteration of foods and beverages. The audience consisted of seventy scientists from Greece and Cyprus working in  Food and Beverage Industry, Govermental Organizations related to Food and Beverage Control and  Greek Universities.
4.Participation  as a co-ordinator or participant in 28 funded research projects by the General Secretary of Greece and European Community (EPAN, PAVET, ENTER, bilateral, HERAKLEITOS, etc).
5.Organization of a partners participating in the MEDEO program which is referred to Development of Novel Methods to Detect Adulteration of Olive Oil by Hazelnut Oil  (November 2001).
6. Participation in COST D22 and COST D26 programs. Acquire SMT mission. 
7. Participation as a co-ordinator in a research project funded by the Institute of Promotion of Research in Cyprus entitled “New Approaches to face Psoriasis” and bilateral collaborations between Cyprus with Slovenia and Romania.
8. Co-organisation with S. Mavromoustakos in Cyprus a scientific conference to honour Prof. D. Poulter (3.11.2008).
9. September 17^th 2010. Co-organisation with Schrodinger a workshop entitled “From Structural Biology to Drug Discovery”. In this workshop 85 researchers and professors from all over Greece participated actively.
10. Chairman with Prof. A. Tsotinis for organizing the international 29th Cyprus-Camerino-Noordwijkerhout Trends in Drug Research ” conference  at Limassol-Cyprus between 2-7 September 2012.
11. Member of the organizing committee of the 32^nd European Conference of Peptide Chemistry ( September Athens 2-7, 2012).
12. Participation as a co-ordinator and participant in 3 funded research projects by Institute of Promotion of Research in Cyprus with University of Cyprus or Institute of Technology.
13. Participation in the Marie Curie Actions EURODAISY. MEST/CT2005/020575. In that action I guided with Dr. M. Papadopoulos S. Durdagi who is now Professor in the Behcesehir University in Istanbul.
14.Participation in COST, EAST-NMR and BIO-NMR programs.
15. Chairman with Prof. A. Tsotinis for organizing the international 29th Cyprus-Camerino-Noordwijkerhout Trends in Drug Research ” conference  at Limassol-Cyprus between 18-22 Μay 2014.
GREEK PATENTS
1. T. Mavromoustakos, G. Bonas, M. Zervou, E. Theodoropoulou. A new method to detect Adulteration of Virgin Olive Oil (No 1002879).
2. T. Mavromoustakos, G. Kokotos, P. Minakaki. Novel Antihypertensive Drugs (No 1004905).
PUBLISHED BOOKS (Greek language)
1. T. Mavromoustakos, J.M. Matsoukas. Nuclear Magnetic Resonance.

• Design of Pharmaceutical Molecules.
• Applications in Medicine, Food and Beverage Chemistry January 1999.

2. T. Mavromoustakos, A. Kolocouris, C. Papakonstantinou, P. Sinigalias, C. Lappas. Two textbooks in Chemistry for the third class of high school . They are published in August of 1998 and are distributed in all high schools of Greece.

3. T. Mavromoustakos, A. Kolocouris, C. Papakonstantinou, P. Sinigalias, C. Lappas. A Chemistry laboratory guide for the third class of high school . They are published in August of 1998 and are distributed in all high schools of Greece.
4. T. Mavromoustakos, A. Kolocouris, C. Papakonstantinou, P. Sinigalias, C. Lappas. Two books containing solutions of the exercises in the textbooks. They are published in August of 1998 and are distributed in all high schools of Greece.
5. T. Mavromoustakos. Use of the NMR in the study of pharmaceutical substances, zeolithes, polymers and archaelogical findings. January 2001.
6. T. Mavromoustakos, J. Matsoukas. NMR spectroscopy . Application in Medicine, Pharmaceutical Chemistry, Biochemistry and Food and Beverage Chemistry (2006).
7. T. Mavromoustakos and P. Zoumpoulakis.. Molecular Modeling. Applications in Organic and Pharmaceutical Chemistry (pages 307) ΙSΒΝ 978-960-89486-5- (2008).
8. T. Mavromoustakos, T. Tselios and C. Papakonstantinou. Fundamental Principles  of Organic Chemistry, Ed. Symmetria, Athens 2014.
Editor
1. Bioactive Peptides in Drug Discovery and Design: Medical Aspects. This book was published by IOS press in January of 1999 as the 22^nd volume of a series of books entitled «Biomedical and Health Research”. Eds. J. Matsoukas, T. Mavromoustakos.
2. Review of Clinical Pharmacology and Pharmacokinetics vol. 11, 1997. An issue dedicated in Peptides and Biomedical Research. Eds. J. Matsoukas, T. Mavromoustakos
3. Bioactive Peptides in Drug Discovery and Design: Medical Aspects.This book is in  press 2002 in as the 55^th volume of a series of books entitled «Biomedical and Health Research”. Eds. J. Matsoukas, T. Mavromoustakos.
4. Guest Editor in Current Reviews in Medicinal Chemistry and more particularly in the issue dedicated to hypertension  («Modern Aspects in the Design and Discovery of Novel Antihypertensive Drugs, 2004». The contributors of this issue included well known scientists of Greece, Europe and USA.
5. Guest Editor in Current Medicinal Chemistyr  volume 18(17), 2011. Methodologies and Applied Strategiew in the Rational Drug Design.