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Near Full-length Genomic Sequencing and Molecular Analysis of HIV-Infected Individuals in a Network-based Intervention (TRIP) in Athens, Greece: Evidence that Transmissions Occur More Frequently from those with High HIV-RNA

Citation:

Kostaki EG, Frampton D, Paraskevis D, Pantavou K, Ferns B, Raffle J, Grant P, Kozlakidis Z, Hadjikou A, Pavlitina E, et al. Near Full-length Genomic Sequencing and Molecular Analysis of HIV-Infected Individuals in a Network-based Intervention (TRIP) in Athens, Greece: Evidence that Transmissions Occur More Frequently from those with High HIV-RNA. Curr HIV ResCurr HIV ResCurr HIV Res. 2018;16:345-353.

Abstract:

BACKGROUND: TRIP (Transmission Reduction Intervention Project) was a network-based, contact tracing approach to locate and link to care, mostly people who inject drugs (PWID) with recent HIV infection. OBJECTIVE: We investigated whether sequences from HIV-infected participants with high viral load cluster together more frequently than what is expected by chance. METHODS: Paired end reads were generated for 104 samples using Illumina MiSeq next-generation sequencing. RESULTS: 63 sequences belonged to previously identified local transmission networks of PWID (LTNs) of an HIV outbreak in Athens, Greece. For two HIV-RNA cut-offs (105 and 106 IU/mL), HIV transmissions were more likely between PWID with similar levels of HIV-RNA (p<0.001). 10 of the 14 sequences (71.4%) from PWID with HIV-RNA >106 IU/mL were clustered in 5 pairs. For 4 of these clusters (80%), there was in each one of them at least one sequence from a recently HIVinfected PWID. CONCLUSION: We showed that transmissions are more likely among PWID with high viremia.

Notes:

1873-4251Kostaki, Evangelia-GeorgiaFrampton, DanielParaskevis, DimitriosPantavou, KaterinaFerns, BridgetRaffle, JadeGrant, PaulKozlakidis, ZisisHadjikou, AndriaPavlitina, EiriniWilliams, Leslie DHatzakis, AngelosFriedman, Samuel RNastouli, EleniNikolopoulos, Georgios KDP1 DA034989/DA/NIDA NIH HHS/United StatesP30 DA011041/DA/NIDA NIH HHS/United StatesJournal ArticleResearch Support, N.I.H., ExtramuralResearch Support, Non-U.S. Gov'tCurr HIV Res. 2018;16(5):345-353. doi: 10.2174/1570162X17666190130120757.