Publication list

2017

Balaska S, Myrianthopoulos V, Tselika M, Hatzinikolaou DG, Mikros E, Diallinas G.NmeA, a novel efflux transporter specific for nucleobases and nucleosides, contributes to metal resistance in Aspergillus nidulans. Mol Microbiol. 2017 Aug;105(3):426-439.

Diallinas G. Transceptors as a functional link of transporters and receptors. Full text | PDF | Microb Cell. 2017 Mar 1;4(3):69-73. doi: 10.15698/mic2017.03.560.

Borbolis F, Flessa CM, Roumelioti F, Diallinas G, Stravopodis DJ, Syntichaki P. Neuronal function of the mRNA decapping complex determines survival of Caenorhabditis elegans at high temperature through temporal regulation of heterochronic gene expression.
Open Biol. 2017 Mar;7(3). pii: 160313. doi: 10.1098/rsob.160313.

Martzoukou O, Amillis S, Zervakou A, Christoforidis S, Diallinas G.The AP-2 complex has a specialized clathrin-independent role in apical endocytosis and polar growth in fungi.Elife. 2017 Feb 21;6. pii: e20083. doi: 10.7554/eLife.20083

de Vries RP, Riley R, Wiebenga A, Aguilar-Osorio G, Amillis S, Uchima CA, Anderluh G, Asadollahi M, Askin M, Barry K, Battaglia E, Bayram Ö, Benocci T, Braus-Stromeyer SA, Caldana C, Cánovas D, Cerqueira GC, Chen F, Chen W, Choi C, Clum A, Dos Santos RA, Damásio AR, Diallinas G, et al. Comparative genomics reveals high biological diversity and specific adaptations in the industrially and medically important fungal genus Aspergillus.Genome Biol. 2017 Feb 14;18(1):28. doi: 10.1186/s13059-017-1151-0.

Sioupouli G, Lambrinidis G, Mikros E, Amillis S, Diallinas G.Cryptic purine transporters in Aspergillus nidulans reveal the role of specific residues in the evolution of specificity in the NCS1 family. Mol Microbiol. 2017 Jan;103(2):319-332. doi: 10.1111/mmi.13559.9.

2016

Alguel Y, Cameron AD, Diallinas G, Byrne B. Transporter oligomerization: form and function. Biochem Soc Trans. 2016 Dec 15;44(6):1737-1744.

Lougiakis N, Gavriil ES, Kairis M, Sioupouli G, Lambrinidis G, Benaki D, Krypotou E, Mikros E, Marakos P, Pouli N, Diallinas G.Design and synthesis of purine analogues as highly specific ligands for FcyB, a ubiquitous fungal nucleobase transporter. Bioorg Med Chem. 2016 Sep 22. pii: S0968-0896(16)30789-1. doi: 10.1016/j.bmc.2016.09.055.

Diallinas G. Dissection of Transporter Function: From Genetics to Structure. Trends Genet. 2016 Jul 15. pii: S0168-9525(16)30064-6. doi: 10.1016/j.tig.2016.06.003.

Alguel Y, Amillis S, Leung J, Lambrinidis G, Capaldi S, Scull NJ, Craven G, Iwata S, Armstrong A, Mikros E, Diallinas G, Cameron AD, Byrne B.Structure of eukaryotic purine/H+ symporter UapA suggests a role for homodimerization in transport activity. Nat Commun. 2016 Apr 18;7:11336.

Evangelinos M, Martzoukou O, Chorozian K, Amillis S, Diallinas G. BsdA(Bsd2) -dependent vacuolar turnover of a misfolded version of the UapA transporter along the secretory pathway: prominent role of selective autophagy. Mol Microbiol. 2016 Jun;100(5):893-911.

2015

Olga Martzoukou, Mayia Karachaliou, Vassilis Yalelis, James Leung, Bernadette Byrne, Sotiris Amillis& George Diallinas. Oligomerization of the UapA purine transporter is critical for ER-exit, plasma membrane localization and turnover. J. Mol Biol. 2015 Aug 14;427(16):2679-96.

Evangelinos M, Anagnostopoulos G, Karvela-Kalogeraki I, Stathopoulou PM, Scazzocchio C, Diallinas G. Minos as a novel Tc1/mariner-type transposable element for functional genomic analysis in Aspergillus nidulans. Fungal Genet Biol. 2015 Aug;81:1-11

Kankipati HN, Rubio-Texeira M, Castermans D, Diallinas G, Thevelein JM. Sul1 and Sul2 Sulfate Transceptors Signal to Protein Kinase A upon Exit of Sulfur Starvation. J Biol Chem. 2015 Apr 17;290(16):10430-46

Krypotou E, Evangelidis T, Bobonis J, Pittis AA, Gabaldón T, Scazzocchio C, Mikros E, Diallinas G. Origin, diversification and substrate specificity in the family of NCS1/FUR transporters. Mol Microbiol. 2015 Jun;96(5):927-50

Sá-Pessoa J, Amillis S, Casal M, Diallinas G. Expression and specificity profile of the major acetate transporter AcpA in Aspergillus nidulans. Fungal Genet Biol. 2015 Mar;76:93-103

Krypotou E, Scazzocchio C, Diallinas G. Functional characterization of NAT/NCS2 proteins of Aspergillus brasiliensis reveals a genuine xanthine-uric acid transporter and an intrinsically misfolded polypeptide. Fungal Genet Biol. 2015 Feb;75:56-63.



2014

Diallinas, G. Understanding transporter specificity and the discrete appearance of channel-like gating domains in transporters. Front. Pharmacol. 2014. 5:207. doi: 10.3389/fphar.2014.00207

Galanopoulou K, Scazzocchio C, Galinou ME, Liu W, Borbolis F, Karachaliou M, Oestreicher N, Hatzinikolaou DG, Diallinas G, Amillis S. Purine utilization proteins in the Eurotiales: cellular compartmentalization, phylogenetic conservation and divergence. Fungal Genet Biol. 2014 Aug;69:96-108. doi:10.1016/j.fgb.2014.06.005.

Krypotou E, Lambrinidis G, Evangelidis T, Mikros E, Diallinas G. Modelling, substrate docking and mutational analysis identify residues essential for function and specificity of the major fungal purine transporter AzgA. Mol Microbiol. 2014 Jul;93(1):129-45. doi: 10.1111/mmi.12646.

Krypotou E, Diallinas G. Transport assays in filamentous fungi: kinetic characterization of the UapC purine transporter of Aspergillus nidulans. Fungal Genet Biol. 2014 Feb;63:1-8. doi: 10.1016/j.fgb.2013.12.004.

2013

Diallinas G. Allopurinol and xanthine use different translocation mechanisms and trajectories in the fungal UapA transporter. Biochimie. 2013 Sep;95(9):1755-64. doi: 10.1016/j.biochi.2013.05.013.

Karachaliou M, Amillis S, Evangelinos M, Kokotos AC, Yalelis V, Diallinas G. The arrestin-like protein ArtA is essential for ubiquitination and endocytosis of the UapA transporter in response to both broad-range and specific signals. Mol Microbiol. 2013 Apr;88(2):301-17. doi: 10.1111/mmi.12184.

Leung J, Cameron AD, Diallinas G, Byrne B. Stabilizing the heterologously expressed uric acid-xanthine transporter UapA from the lower eukaryote Aspergillus nidulans. Mol Membr Biol. 2013 Feb;30(1):32-42. doi: 10.3109/09687688.2012.690572.

2012

Krypotou E, Kosti V, Amillis S, Myrianthopoulos V, Mikros E, Diallinas G. Modeling, substrate docking, and mutational analysis identify residues essential  for the function and specificity of a eukaryotic purine-cytosine NCS1 transporter. J Biol Chem. 2012 Oct 26;287(44):36792-803. doi: 10.1074/jbc.M112.400382.

Kosti V, Lambrinidis G, Myrianthopoulos V, Diallinas G, Mikros E. Identification of the substrate recognition and transport pathway in a eukaryotic member of the nucleobase-ascorbate transporter (NAT) family. PLoS One. 2012;7(7):e41939. doi: 10.1371/journal.pone.0041939

Takeshita N, Diallinas G, Fischer R. The role of flotillin FloA and stomatin StoA in the maintenance of apical sterol-rich membrane domains and polarity in the filamentous fungus Aspergillus nidulans. Mol Microbiol. 2012 Mar;83(6):1136-52. doi: 10.1111/j.1365-2958.2012.07996.x

2011

Amillis S, Kosti V, Pantazopoulou A, Mikros E, Diallinas G. Mutational analysis and modeling reveal functionally critical residues in transmembrane segments 1 and 3 of the UapA transporter. J Mol Biol. 2011 Aug 19;411(3):567-80.  doi: 10.1016/j.jmb.2011.06.024.

Soares-Silva I, Sá-Pessoa J, Myrianthopoulos V, Mikros E, Casal M, Diallinas  G. A substrate translocation trajectory in a cytoplasm-facing topological model of the monocarboxylate/H⁺ symporter Jen1p. Mol Microbiol. 2011 Aug;81(3):805-17.  doi: 10.1111/j.1365-2958.2011.07729.x.

Gournas C, Oestreicher N, Amillis S, Diallinas G, Scazzocchio C. Completing the purine utilisation pathway of Aspergillus nidulans. Fungal Genet Biol. 2011 Aug;48(8):840-8. doi: 10.1016/j.fgb.2011.03.004.

Anasontzis GE, Zerva A, Stathopoulou PM, Haralampidis K, Diallinas G, Karagouni AD, Hatzinikolaou DG. Homologous overexpression of xylanase in Fusarium oxysporum increases ethanol productivity during consolidated bioprocessing (CBP)  of lignocellulosics. J Biotechnol. 2011 Mar 10;152(1-2):16-23. doi: 10.1016/j.jbiotec.2011.01.002.

Bitsikas V, Karachaliou M, Gournas C, Diallinas G. Hypertonic conditions trigger transient plasmolysis, growth arrest and blockage of transporter endocytosis in Aspergillus nidulans and Saccharomyces cerevisiae. Mol Membr Biol. 2011 Jan;28(1):54-68. doi: 10.3109/09687688.2010.510484

2010

Elsen S, Efthymiou G, Petein Leung J, Cameron AD, Diallinas G, Byrne B. Stabilizing the heterologously expressed uric acid-xanthine transporter UapA from the lower eukaryote Aspergillus nidulans. Mol Membr Biol. 2013 Feb;30(1):32-42. doi: 10.3109/09687688.2012.690572.

Petinatos P, Diallinas G, Kyritsis P, Moulis JM. A bacteria-specific 2[4Fe-4S] ferredoxin is essential in Pseudomonas aeruginosa. BMC Microbiol. 2010 Oct 28;10:271. doi: 10.1186/1471-2180-10-271.

Kosti V, Papageorgiou I, Diallinas G. Dynamic elements at both cytoplasmically and extracellularly facing sides of the UapA transporter selectively control the accessibility of substrates to their translocation pathway. J Mol Biol. 2010 Apr 16;397(5):1132-43. doi: 10.1016/j.jmb.2010.02.037.

Leung J, Karachaliou M, Alves C, Diallinas G, Byrne B. Expression and purification of a functional uric acid-xanthine transporter (UapA). Protein Expr  Purif. 2010 Jul;72(1):139-46. doi: 10.1016/j.pep.2010.02.002.

2009

Conde A, Diallinas G, Chaumont F, Chaves M, Gerós H. Transporters, channels,  or simple diffusion? Dogmas, atypical roles and complexity in transport systems.  Int J Biochem Cell Biol. 2010 Jun;42(6):857-68. doi: 10.1016/j.biocel.2009.12.012.

Gournas C, Amillis S, Vlanti A, Diallinas G. Transport-dependent endocytosis  and turnover of a uric acid-xanthine permease. Mol Microbiol. 2010 Jan;75(1):246-60. doi: 10.1111/j.1365-2958.2009.06997.x

Vangelatos I, Vlachakis D, Sophianopoulou V, Diallinas G. Modelling and mutational evidence identify the substrate binding site and functional elements in APC amino acid transporters. Mol Membr Biol. 2009 Aug;26(5):356-70. doi:10.1080/09687680903170546.

Hamari Z, Amillis S, Drevet C, Apostolaki A, Vágvölgyi C, Diallinas G, Scazzocchio C. Convergent evolution and orphan genes in the Fur4p-like family and characterization of a general nucleoside transporter in Aspergillus nidulans. Mol Microbiol. 2009 Jul;73(1):43-57. doi: 10.1111/j.1365-2958.2009.06738.x.

2008

Diallinas G. Biochemistry. An almost-complete movie. Science. 2008 Dec 12;322(5908):1644-5. doi: 10.1126/science.1168107.

Diallinas G, Gournas C. Structure-function relationships in the nucleobase-ascorbate transporter (NAT) family: lessons from model microbial genetic systems. Channels (Austin). 2008 Sep-Oct;2(5):363-72.

Lemuh ND, Diallinas G, Frillingos S, Mermelekas G, Karagouni AD, Hatzinikolaou DG. Purification and partial characterization of the xanthine-uric  acid transporter (UapA) of Aspergillus nidulans. Protein Expr Purif. 2009 Jan;63(1):33-9. doi: 10.1016/j.pep.2008.08.012.

Papageorgiou I, Gournas C, Vlanti A, Amillis S, Pantazopoulou A, Diallinas G. Specific interdomain synergy in the UapA transporter determines its unique specificity for uric acid among NAT carriers. J Mol Biol. 2008 Oct 24;382(5):1121-35. doi: 10.1016/j.jmb.2008.08.005.

Gournas C, Papageorgiou I, Diallinas G. The nucleobase-ascorbate transporter  (NAT) family: genomics, evolution, structure-function relationships and physiological role. Mol Biosyst. 2008 May;4(5):404-16. doi: 10.1039/b719777b.

Vlanti A, Diallinas G. The Aspergillus nidulans FcyB cytosine-purine scavenger is highly expressed during germination and in reproductive compartments and is downregulated by endocytosis. Mol Microbiol. 2008 May;68(4):959-77. doi: 10.1111/j.1365-2958.2008.06198.x.

Papageorgiou I, De Koning HP, Soteriadou K, Diallinas G. Kinetic and mutational analysis of the Trypanosoma brucei NBT1 nucleobase transporter expressed in Saccharomyces cerevisiae reveals structural similarities between ENT and MFS transporters. Int J Parasitol. 2008 May;38(6):641-53.

2007

Goudela S, Reichard U, Amillis S, Diallinas G. Characterization and kinetics  of the major purine transporters in Aspergillus fumigatus. Fungal Genet Biol. 2008 Apr;45(4):459-72.

Pantazopoulou A, Diallinas G. Fungal nucleobase transporters. FEMS Microbiol  Rev. 2007 Nov;31(6):657-75.

Soares-Silva I, Paiva S, Diallinas G, Casal M. The conserved sequence NXX[S/T]HX[S/T]QDXXXT of the lactate/pyruvate:H(+) symporter subfamily defines the function of the substrate translocation pathway. Mol Membr Biol. 2007 Sep-Dec;24(5-6):464-74.

Amillis S, Hamari Z, Roumelioti K, Scazzocchio C, Diallinas G. Regulation of  expression and kinetic modeling of substrate interactions of a uracil transporter in Aspergillus nidulans. Mol Membr Biol. 2007 May-Jun;24(3):206-14.

Pantazopoulou A, Lemuh ND, Hatzinikolaou DG, Drevet C, Cecchetto G, Scazzocchio C, Diallinas G. Differential physiological and developmental expression of the UapA and AzgA purine transporters in Aspergillus nidulans. Fungal Genet Biol. 2007 Jul;44(7):627-40.

2006

Pantazopoulou A, Diallinas G. The first transmembrane segment (TMS1) of UapA  contains determinants necessary for expression in the plasma membrane and purine  transport. Mol Membr Biol. 2006 Jul-Aug;23(4):337-48. PubMed PMID: 16923727.

Goudela S, Tsilivi H, Diallinas G. Comparative kinetic analysis of AzgA and Fcy21p, prototypes of the two major fungal hypoxanthine-adenine-guanine transporter families. Mol Membr Biol. 2006 Jul-Aug;23(4):291-303.

Vlanti A, Amillis S, Koukaki M, Diallinas G. A novel-type substrate-selectivity filter and ER-exit determinants in the UapA purine transporter. J Mol Biol. 2006 Mar 31;357(3):808-19.

2005

Goudela S, Karatza P, Koukaki M, Frillingos S, Diallinas G. Comparative substrate recognition by bacterial and fungal purine transporters of the NAT/NCS2 family. Mol Membr Biol. 2005 May-Jun;22(3):263-75.

Koukaki M, Vlanti A, Goudela S, Pantazopoulou A, Gioule H, Tournaviti S, Diallinas G. The nucleobase-ascorbate transporter (NAT) signature motif in UapA defines the function of the purine translocation pathway. J Mol Biol. 2005 Jul 15;350(3):499-513.

Papageorgiou IG, Yakob L, Al Salabi MI, Diallinas G, Soteriadou KP, De Koning HP. Identification of the first pyrimidine nucleobase transporter in Leishmania:  similarities with the Trypanosoma brucei U1 transporter and antileishmanial activity of uracil analogues. Parasitology. 2005 Mar;130(Pt 3):275-83.

2004

Amillis S, Cecchetto G, Sophianopoulou V, Koukaki M, Scazzocchio C, Diallinas G. Transcription of purine transporter genes is activated during the isotropic growth phase of Aspergillus nidulans conidia. Mol Microbiol. 2004 Apr;52(1):205-16.

Cecchetto G, Amillis S, Diallinas G, Scazzocchio C, Drevet C. The AzgA purine transporter of Aspergillus nidulans. Characterization of a protein belonging to a new phylogenetic cluster. J Biol Chem. 2004 Jan 30;279(5):3132-41.

2003

Tavoularis SN, Tazebay UH, Diallinas G, Sideridou M, Rosa A, Scazzocchio C, Sophianopoulou V. Mutational analysis of the major proline transporter (PrnB) of  Aspergillus nidulans. Mol Membr Biol. 2003 Oct-Dec;20(4):285-97.

Koukaki M, Giannoutsou E, Karagouni A, Diallinas G. A novel improved method for Aspergillus nidulans transformation. J Microbiol Methods. 2003Dec;55(3):687-95.

2001

Amillis S, Koukaki M, Diallinas G. Substitution F569S converts UapA, a specific uric acid-xanthine transporter, into a broad specificity transporter for purine-related solutes. J Mol Biol. 2001 Nov 2;313(4):765-74.

Ververidis P, Davrazou F, Diallinas G, Georgakopoulos D, Kanellis AK, Panopoulos N. A novel putative reductase (Cpd1p) and the multidrug exporter Snq2p are involved in resistance to cercosporin and other singlet oxygen-generatingphotosensitizers in Saccharomyces cerevisiae. Curr Genet. 2001 May;39(3):127-36.

 

Argyrou E, Sophianopoulou V, Schultes N, Diallinas G. Functional characterization of a maize purine transporter by expression in Aspergillus nidulans. Plant Cell. 2001 Apr;13(4):953-64.

2000

Valdez-Taubas J, Diallinas G, Scazzocchio C, Rosa AL. Protein expression and  subcellular localization of the general purine transporter UapC from Aspergillus  nidulans. Fungal Genet Biol. 2000 Jul;30(2):105-13.

de Koning H, Diallinas G. Nucleobase transporters (review). Mol Membr Biol. 2000 Apr-Jun;17(2):75-94.

Meintanis C, Karagouni AD, Diallinas G. Amino acid residues N450 and Q449 are critical for the uptake capacity and specificity of UapA, a prototype of a nucleobase-ascorbate transporter family. Mol Membr Biol. 2000 Jan-Mar;17(1):47-57.

1998

Diallinas G, Valdez J, Sophianopoulou V, Rosa A, Scazzocchio C. Chimeric purine transporters of Aspergillus nidulans define a domain critical for function and specificity conserved in bacterial, plant and metazoan homologues. EMBO J. 1998 Jul 15;17(14):3827-37.

1997

Diallinas G, Pateraki I, Sanmartin M, Scossa A, Stilianou E, Panopoulos NJ, Kanellis AK. Melon ascorbate oxidase: cloning of a multigene family, induction during fruit development and repression by wounding. Plant Mol Biol. 1997 Jul;34(5):759-70.

Ravagnani A, Gorfinkiel L, Langdon T, Diallinas G, Adjadj E, Demais S, Gorton D, Arst HN Jr, Scazzocchio C. Subtle hydrophobic interactions between the seventh residue of the zinc finger loop and the first base of an HGATAR sequence determine promoter-specific recognition by the Aspergillus nidulans GATA factor AreA. EMBO J. 1997 Jul 1;16(13):3974-86

Tazebay UH, Sophianopoulou V, Scazzocchio C, Diallinas G. The gene encoding the major proline transporter of Aspergillus nidulans is upregulated during conidiospore germination and in response to proline induction and amino acid starvation. Mol Microbiol. 1997 Apr;24(1):105-17.

1995

Tazebay UH, Sophianopoulou V, Cubero B, Scazzocchio C, Diallinas G. Post-transcriptional control and kinetic characterization of proline transport in germinating conidiospores of Aspergillus nidulans. FEMS Microbiol Lett. 1995 Oct  1;132(1-2):27-37.

Diallinas G, Gorfinkiel L, Arst HN Jr, Cecchetto G, Scazzocchio C. Genetic and molecular characterization of a gene encoding a wide specificity purine permease of Aspergillus nidulans reveals a novel family of transporters conserved in prokaryotes and eukaryotes. J Biol Chem. 1995 Apr 14;270(15):8610-22.

Sophianopoulou V, Diallinas G. Amino acid transporters of lower eukaryotes: regulation, structure and topogenesis. FEMS Microbiol Rev. 1995 Jan;16(1):53-75.

 

1994

Diallinas G, Kanellis AK. A phenylalanine ammonia-lyase gene from melon fruit: cDNA cloning, sequence and expression in response to development and wounding. Plant Mol Biol. 1994 Oct;26(1):473-9.  

Diallinas G, Thireos G. Genetic and biochemical evidence for yeast GCN2 protein kinase polymerization. Gene. 1994 May 27;143(1):21-7

Tazebay U, Sophianopoulou V, Rosa A, Scazzocchio C, Diallinas G. Structure-function analysis of the proline permease (PRNB) of the filamentous fungus Aspergillus nidulans. Folia Microbiol (Praha). 1994;39(6):551

Diallinas G, Gorfinkiel L, Arst HN Jr, Cecchetto G, Scazzocchio C. Genetic and molecular characterisation of purine permease genes of Aspergillus nidulans reveals a novel family of transporters conserved in prokaryotes and eukaryotes. Folia Microbiol (Praha). 1994;39(6):513-4.

1993

Gorfinkiel L, Diallinas G, Scazzocchio C. Sequence and regulation of the uapA gene encoding a uric acid-xanthine permease in the fungus Aspergillus nidulans. J Biol Chem. 1993 Nov 5;268(31):23376-81.

Sophianopoulou V, Diallinas G. AUA1, a gene involved in ammonia regulation of amino acid transport in Saccharomyces cerevisiae. Mol Microbiol. 1993 Apr;8(1):167-78.

Sophianopoulou V, Suárez T, Diallinas G, Scazzocchio C. Operator derepressed  mutations in the proline utilisation gene cluster of Aspergillus nidulans. Mol Gen Genet. 1993 Jan;236(2-3):209-13.

1989

Diallinas G, Scazzocchio C. A gene coding for the uric acid-xanthine permease of Aspergillus nidulans: inactivational cloning, characterization, and sequence of a cis-acting mutation. Genetics. 1989 Jun;122(2):341-50.

 

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