The AP-1 complex is essential for membrane protein traffic via its role in the pinching-off and sorting of secretory vesicles from the trans-Golgi and/or endosomes. While its essentiality is undisputed in metazoa, its role in simpler eukaryotes seems less clear. Here we dissect the role of AP-1 in the filamentous fungus and show that it is absolutely essential for growth due to its role in clathrin-dependent maintenance of polar traffic of specific membrane cargoes towards the apex of growing hyphae. We provide evidence that AP-1 is involved in both anterograde sorting of RabE-labeled secretory vesicles and RabA/B-dependent endosome recycling. Additionally, AP-1 is shown to be critical for microtubule and septin organization, further rationalizing its essentiality in cells that face the challenge of cytoskeleton-dependent polarized cargo traffic. This work also opens a novel issue on how non-polar cargoes, such as transporters, are sorted to the eukaryotic plasma membrane.
A large amount of new and existing data was applied, aiming at delineating structural seismic risk in the earthquake prone modern city of Kalamata (SW Peloponnese) that was largely reconstructed after the devastating Mw=5.8 earthquake of September 13, 1986. Synthetic site-specifc ground motion parameters derived from the nearest known shallow hazardous seismogenic sources were combined with EMS-98 structural vulnerability estimates of the city’s building stock and four structural and economic loss models have been developed on a building-block scale. Assuming absence of non-linear, nearsource effects, the building stock of Kalamata is anticipated to present sufficient seismic behaviour, due to the large number of new and innovative constructions replacing the demolished ones after the 1986 earthquake.
Natural products are characterized by extreme structural diversity and thus they offer a unique source for the identification of novel anti-tumor agents. Herein, we report that the herbal substance acteoside being isolated by advanced phytochemical methods from Lippia citriodora leaves showed enhanced cytotoxicity against metastatic tumor cells; acted in synergy with various cytotoxic agents and it sensitized chemoresistant cancer cells. Acteoside was not toxic in physiological cellular contexts, while it increased oxidative load, affected the activity of proteostatic modules and suppressed matrix metalloproteinases in tumor cell lines. Intraperitoneal or oral (via drinking water) administration of acteoside in a melanoma mouse model upregulated antioxidant responses in the tumors; yet, only intraperitoneal delivery suppressed tumor growth and induced anti-tumor-reactive immune responses. Mass-spectrometry identification/quantitation analyses revealed that intraperitoneal delivery of acteoside resulted in significantly higher, vs. oral administration, concentration of the compound in the plasma and tumors of treated mice, suggesting that its in vivo anti-tumor effect depends on the route of administration and the achieved concentration in the tumor. Finally, molecular modeling studies and enzymatic activity assays showed that acteoside inhibits protein kinase C. Conclusively, acteoside holds promise as a chemical scaffold for the development of novel anti-tumor agents.
Aromatase inhibitors, which are widely used for the treatment of estrogen-dependent cancers, have been associated with psychiatric side effects ranging from mania to depression. In the present study, we investigated sex differences in the behavioral and neurochemical effects of aromatase inhibition on male and female, sham-operated or gonadectomized adult rats. Three weeks after surgery, rats received chronic treatment with the aromatase inhibitor letrozole or vehicle and were then subjected to the open field test, which assesses general activity. Half of the subjects were subsequently exposed to the stressful procedure of the forced swim test (FST), which is also a test of antidepressant activity. Aromatase activity was analyzed in the hypothalamus and testosterone and corticosterone were assayed in the blood serum of all rats. The hippocampus and prefrontal cortex (PFC) were analyzed for monoamine (noradrenaline, dopamine and serotonin), as well as amino acid (GABA, glutamate, glycine, taurine, alanine and histidine) levels. The observed decrease in hypothalamic aromatase activity confirmed the efficacy of letrozole treatment in both sexes. Moreover, letrozole enhanced testosterone levels in sham-operated females. In the open field test, females were overall more active and explorative than males and gonadectomy eliminated this sex difference. In the FST, females exhibited overall higher immobility than males and gonadectomy further enhanced this passive behavior in both sexes. However, sustained aromatase inhibition had no effect on open field and FST behaviors. Head shakes during FST, which were fewer in females than in males, were reduced by castration in males and by letrozole treatment in ovariectomized females, suggesting a role of testosterone and extra-gonadal estrogens in the expression of this behavior. Sustained aromatase inhibition also decreased noradrenaline and the dopaminergic turnover rates [DOPAC/DA, HVA/DA] in the hippocampus and PFC of male and female rats, irrespectively of gonadectomy. Moreover, letrozole treatment enhanced the serotonergic turnover [5HIAA/5HT] rate in the hippocampus of males and females, irrespectively of gonadectomy. Amino acid levels were not influenced by letrozole, but sex differences were demonstrated with higher levels in the PFC of females vs. males. Present findings suggest that the neuropsychiatric effects of aromatase inhibition can be attributed to the inhibition of extragonadal estrogen synthesis, presumably in the brain, and could be further associated with serotonergic and catecholaminergic changes in brain regions involved in mood and cognition. Importantly, present data could be linked with the neurobiology of affective side-effects in post-menopausal women receiving aromatase inhibitors.
Aromatase inhibitors, which are widely used for the treatment of estrogen-dependent cancers, have been associated with psychiatric side effects ranging from mania to depression. In the present study, we investigated sex differences in the behavioral and neurochemical effects of aromatase inhibition on male and female, sham-operated or gonadectomized adult rats. Three weeks after surgery, rats received chronic treatment with the aromatase inhibitor letrozole or vehicle and were then subjected to the open field test, which assesses general activity. Half of the subjects were subsequently exposed to the stressful procedure of the forced swim test (FST), which is also a test of antidepressant activity. Aromatase activity was analyzed in the hypothalamus and testosterone and corticosterone were assayed in the blood serum of all rats. The hippocampus and prefrontal cortex (PFC) were analyzed for monoamine (noradrenaline, dopamine and serotonin), as well as amino acid (GABA, glutamate, glycine, taurine, alanine and histidine) levels. The observed decrease in hypothalamic aromatase activity confirmed the efficacy of letrozole treatment in both sexes. Moreover, letrozole enhanced testosterone levels in sham-operated females. In the open field test, females were overall more active and explorative than males and gonadectomy eliminated this sex difference. In the FST, females exhibited overall higher immobility than males and gonadectomy further enhanced this passive behavior in both sexes. However, sustained aromatase inhibition had no effect on open field and FST behaviors. Head shakes during FST, which were fewer in females than in males, were reduced by castration in males and by letrozole treatment in ovariectomized females, suggesting a role of testosterone and extra-gonadal estrogens in the expression of this behavior. Sustained aromatase inhibition also decreased noradrenaline and the dopaminergic turnover rates [DOPAC/DA, HVA/DA] in the hippocampus and PFC of male and female rats, irrespectively of gonadectomy. Moreover, letrozole treatment enhanced the serotonergic turnover [5HIAA/5HT] rate in the hippocampus of males and females, irrespectively of gonadectomy. Amino acid levels were not influenced by letrozole, but sex differences were demonstrated with higher levels in the PFC of females vs. males. Present findings suggest that the neuropsychiatric effects of aromatase inhibition can be attributed to the inhibition of extragonadal estrogen synthesis, presumably in the brain, and could be further associated with serotonergic and catecholaminergic changes in brain regions involved in mood and cognition. Importantly, present data could be linked with the neurobiology of affective side-effects in post-menopausal women receiving aromatase inhibitors.
BACKGROUND: Eastern European countries including Russia, Ukraine and other former Soviet Union (FSU) countries have experienced an HIV epidemic spreading mostly among people who inject drugs (PWID). We aimed to investigate the origin and the dispersal patterns of HIV-1 CRF02_AG in Russia and other FSU countries.
METHODS: We studied 136 CRF02_AG sequences originating from Russia, and FSU countries along with a globally sampled dataset of 3,580 CRF02_AG sequences. Maximum-likelihood phylogeny reconstruction with bootstrap evaluation was conducted in RAxML. Bayesian phylogeographic analysis was performed in BEAST v1.8 using the discrete trait model.
RESULTS: We found that all CRF02_AG sequences from Russia and other FSU countries formed a single monophyletic cluster within CRF02_AG radiation. The Russian/FSU clade was classified as CRF63_02A1. Sequences from the FSU countries clustered further within distinct subclades (two from Russia, three from Uzbekistan and one Kazakhstan) according to the geographic origin of sampling. Molecular clock analysis revealed that the tMRCA of the CRF63_02A1 epidemic was in 1996 (95%HPD: 1992-1999), while for the two Russian subclades, tMRCA was estimated in 2003 (95%HPD: 2001-2004) and in 2007 (95%HPD: 2005-2008). Phylogeographic analysis suggested that the potential origin of the epidemic was in Uzbekistan. Early dispersal of CRF63_02A1 occurred in Kazakhstan and Uzbekistan and thereafter the epidemic spread to Russia. Notably, spillover transmissions to Russia kept occurring in both countries.
DISCUSSION: Previous studies have shown that Russia and Ukraine have provided the source for the PWID-driven, HIV-1 subtype-A epidemic, spreading across the FSU countries (AFSU). In great contrast, CRF63_02A1 established an epidemic in central Asia (Uzbekistan and Kazakhstan), from where it subsequently disseminated to Russia. Our study suggests that cross-border transmissions among PWID occur bi-directionally between Russian and other FSU populations. These results are of public health importance and suggest that prevention actions have to be reinforced in this area to assist the management of high-risk practices.
We report on the electronic structure, density of states and transmission properties of the periodic one-dimensional tight-binding (TB) lattice with a single orbital per site and nearest-neighbor hoppings, with a generic unit cell of u sites. The determination of the eigenvalues is equivalent to the diagonalization of a real tridiagonal symmetric u-Toeplitz matrix with (cyclic boundaries) or without (fixed boundaries) perturbed upper right and lower left corners. We solve the TB equations via the Transfer Matrix Method, producing analytical solutions and recursive relations for its eigenvalues, closely related to the Chebyshev polynomials. We examine the density of states and provide relevant analytical relations. We attach semi-infinite leads, determine and discuss the transmission coefficient at zero bias and investigate the peaks number and position, and the effect of the coupling strength and asymmetry as well as of the lead properties on the transmission profiles. We introduce a generic optimal coupling condition and demonstrate its physical meaning.
Following the discovery of the newly discovered ULX pulsar in NGC 300 (ATel #11158) we searched the available X-ray data for the evolution of the spin period of the neutron star and the X-ray luminosity.
The semimetal MoTe2 is studied by spin- and angle-resolved photoemission spectroscopy across the centrosymmetry-breaking structural transition temperature of the bulk. A three-dimensional spin- texture is observed in the bulk Fermi surface in the low temperature, noncentrosymmetric phase that is consistent with first-principles calculations. The spin texture and two types of surface Fermi arc are not completely suppressed above the bulk transition temperature. The lifetimes of quasiparticles forming the Fermi arcs depend on thermal history and lengthen considerably upon cooling toward the bulk structural transition. The results indicate that a new form of polar instability exists near the surface when the bulk is largely in a centrosymmetric phase.
We consider a state-dependent single-server queue with orbit. This is a versatile model for the study of service systems, where the server needs a non-negligible time to retrieve waiting customers every time he completes a service. This situation arises typically when the customers are not physically present at a system, but they have a remote access to it, as in a call center station, a communication node, etc. We introduce a probabilistic approach for the performance evaluation of this queueing system, that we refer to as the queueing and Markov chain decomposition approach. Moreover, we discuss the applicability of this approach for the performance evaluation of other non-Markovian service systems with state dependencies.
The Indian summer monsoon (ISM) is a prominent feature of the summer circulation in the Northern Hemisphere (NH) and has been found to modulate the weather and climate conditions in many remote regions. This study investigates the most recurrent patterns of summertime midlatitude circulation, over the eastern Mediterranean (EM) and also globally, that are most associated with the ISM. Monthly data of 44 summers from the ERA40 dataset are used and two multidimensional statistical methods, the Principal Component Analysis (PCA) and Canonical Correlation Analysis (CCA), are implemented. The ISM is found to be related to subsidence anomalies in the middle and more extendedly in the upper troposphere over the central and eastern Mediterranean and with an Etesian-like pattern regarding the field of the lower troposphere winds. An equatorial Rossby wave pattern, extending westward from an ISM heat source up to EM and N. Africa, was identified to be associated with the variability of ISM. The observed relationship between the ISM and the EM circulation features can be attributed to this equatorial Rossby wave response to the monsoon forcing. CCA implementation revealed the interconnection of the aforementioned PCA results with an ISM action center over the northern Arabian Sea and the monsoon trough region.
Magnetic reconnection is invoked as an efficient particle accelerator in a variety of astrophysical sources of non-thermal high-energy radiation. With large-scale two-dimensional particle-in-cell simulations of relativistic reconnection (i.e. with magnetization σ ≫ 1) in pair plasmas, we study the long-term evolution of the power-law slope and high-energy cut-off of the spectrum of accelerated particles. We find that the high-energy spectral cut-off does not saturate at γcut ∼ 4σ, as claimed by earlier studies, but it steadily grows with time as long as the reconnection process stays active. At late times, the cut-off scales approximately as γ _cut∝ √{t}, regardless of the flow magnetization and initial temperature. We show that the particles dominating the high-energy spectral cut-off reside in plasmoids, and in particular in a strongly magnetized ring around the plasmoid core. The growth of their energy is driven by the increase in the local field strength, coupled with the conservation of the first adiabatic invariant. We also find that the power-law slope of the spectrum (p = -d log N/d log γ) evolves with time. For σ ≳ 10, the spectrum is hard at early times (p ≲ 2), but it tends to asymptote to p ∼ 2; the steepening of the power-law slope allows the spectral cut-off to extend to higher and higher energies, without violating the fixed energy budget of the system. Our results demonstrate that relativistic reconnection is a viable candidate for accelerating the high-energy particles emitting in relativistic astrophysical sources.
A series of uranium(III) mixed sandwich complexes with sterically demanding CpR ligands, of the type [U(COTTIPS2)(CpR)] (CpR = CptBu (C5H4tBu), CptBu2 (C5H3tBu2-1,3), CptBu3 (C5H2tBu3-1,2,4), CpTIPS2 (C5H3(SiiPr3)2-1,3), CpMe4Bz (C5Me4CH2Ph), IndMe6 (C9HMe6) and IndMe7 (C9Me7), and COTTIPS2 = C8H6(SiiPr3)2-1,4), have been synthesised and their X-ray crystal structures determined. The reactivity of these complexes with CO and CO2 is reported, including the squarate complex [U(COTTIPS2)(IndMe6)]2(μ-C4O4), IR data on the long-lived carbonyl complex [U(COTTIPS2)(IndMe7)(CO)] and the carbonate complex [U(COTTIPS2)(CptBu)]2(μ-η1:η2-CO3). The Solid-G algorithm has been to assess the steric properties of these and previously reported mixed-sandwich complexes in the solid state and correlate these properties with the observed reactivity.
We present the results of our power spectral density analysis for the BL Lac object OJ 287, utilizing the Fermi-LAT survey at high-energy γ-rays, Swift-XRT in X-rays, several ground-based telescopes and the Kepler satellite in the optical, and radio telescopes at GHz frequencies. The light curves are modeled in terms of continuous-time autoregressive moving average (CARMA) processes. Owing to the inclusion of the Kepler data, we were able to construct for the first time the optical variability power spectrum of a blazar without any gaps across ∼6 dex in temporal frequencies. Our analysis reveals that the radio power spectra are of a colored-noise type on timescales ranging from tens of years down to months, with no evidence for breaks or other spectral features. The overall optical power spectrum is also consistent with a colored noise on the variability timescales ranging from 117 years down to hours, with no hints of any quasi-periodic oscillations. The X-ray power spectrum resembles the radio and optical power spectra on the analogous timescales ranging from tens of years down to months. Finally, the γ-ray power spectrum is noticeably different from the radio, optical, and X-ray power spectra of the source: we have detected a characteristic relaxation timescale in the Fermi-LAT data, corresponding to ∼150 days, such that on timescales longer than this, the power spectrum is consistent with uncorrelated (white) noise, while on shorter variability timescales there is correlated (colored) noise.
Montenegro plans to enhance its educational system in the area of health information management, in accordance with well-known EU best practices. Within the Erasmus+ project PH-ELIM, a Stratified Framework was developed to provide education of public health professionals making them highly skilled to support the nation in creating a sustainable and flexible health system, in providing good quality health, in protecting citizens against health threats, all by a cost-effective and straightforward approach. The objective of this presentation is to present the intermediate results of the Framework and lessons learned until now.
Schizotypal personality traits may increase proneness to psychosis and likely index familial vulnerability to schizophrenia (SZ), implying shared genetic determinants with SZ. Here, we sought to investigate the contribution of common genetic risk variation for SZ on self-reported schizotypy in 2 ethnically homogeneous cohorts of healthy young males during compulsory military service, enrolled in the Athens Study of Proneness and Incidence of Schizophrenia (ASPIS, N = 875) and the Learning on Genetics of Schizophrenia Spectrum study (LOGOS, N = 690). A follow-up psychometric assessment was performed in a sub-sample of the ASPIS (N = 121), 18 months later at military service completion. Polygenic risk scores (PRS) for SZ were derived based on genome-wide association meta-analysis results from the Psychiatric Genomics Consortium. In the ASPIS, higher PRSSZ significantly associated with lower levels of positive (ie, perceptual distortions), disorganization and paranoid facets of schizotypy, whereas no association with negative (ie, interpersonal) facets was noted. Importantly, longitudinal data analysis in the ASPIS subsample revealed that PRSSZ was inversely associated with positive schizotypy at military induction (stressed condition) but not at follow-up (nonstressed condition), providing evidence for environmental rather than SZ-implicated genetic influences. Moreover, consistent with prior reports, PRSSZ was positively correlated with trait anxiety in the LOGOS and additionally the recruits with higher PRSSZ and trait anxiety exhibited attenuated paranoid ideation. Together, these findings do not support an etiological link between increased polygenic liability for SZ and schizotypy, suggesting that psychosocial stress or trait anxiety may impact schizotypal phenotypic expressions among healthy young adults not genetically predisposed to SZ.
The role of membrane lipids in modulating eukaryotic transporter assembly and function remains unclear. We investigated the effect of membrane lipids in the structure and transport activity of the purine transporter UapA from Aspergillus nidulans. We found that UapA exists mainly as a dimer and that two lipid molecules bind per UapA dimer. We identified three phospholipid classes that co-purified with UapA: phosphatidylcholine, phosphatidylethanolamine (PE), and phosphatidylinositol (PI). UapA delipidation caused dissociation of the dimer into monomers. Subsequent addition of PI or PE rescued the UapA dimer and allowed recovery of bound lipids, suggesting a central role of these lipids in stabilizing the dimer. Molecular dynamics simulations predicted a lipid binding site near the UapA dimer interface. Mutational analyses established that lipid binding at this site is essential for formation of functional UapA dimers. We propose that structural lipids have a central role in the formation of functional, dimeric UapA.
In this paper, we explore the process of becoming a teacher educator in the pedagogical use of digital tools in mathematics teaching. The study took place in the context of an in-service program during the trainees’ engagement in their practicum fieldwork activities including the process observation–reflection–design–implementation–reflection. We explored the features of this context that facilitated the trainees’ transition from the level of trainee educator to the level of teacher educator as well as the nature of the trainees’ documentation work for teachers. The results showed that observation of other teacher educators’ teaching in conjunction with reflection during the program’s respective sessions facilitated the trainees’ transition to the professional level. The identified operational invariants underlying the trainees’ designs concerned the focus of their observation in teacher education classrooms, the importance they attributed to the constraints and opportunities provided by the wider educational context and epistemological issues regarding the teaching and learning of mathematics with technology. The analysis of trainees’ designs revealed three kinds of documents (‘‘explanatory,’’ ‘‘instructive’’ and ‘‘facilitative’’) and corresponding roles of trainees during the implementation. These documents targeted different aspects of TPACK depending on the trainees’ conceptualizations of teachers’ roles either ‘‘as students’’ or ‘‘of students.’’
Electric-field-controlled piezoelectric strain has been used, recently, to modify the superconducting properties in a new class of piezoelectric/superconducting (PE/SC) hybrids. Here, we investigate the appearance of thermomagnetic instabilities (TMIs) and the respective modification of the critical current density (J(C)) through the application of electric field (E-ex) in PE/SC hybrids. Specifically, the SC nanolayers are Nb (thickness, d(SC) = 20 nm) deposited on both surfaces of PE macroscopic crystals of (1-x)Pb(Mg-1/3 Nb-2/3)O-3-xPbTiO(3) (PMN-PT) with optimum composition x = 0.31 (thickness, d(PE) = 0.5-0.8 mm). The appearance of TMIs and the modification of J(C) by E-ex is studied for two PMN-PT crystals of drastically different surface roughness (Sa). In the case of the PMN-PT crystal with low Sa (on the order of a few tenths of nm) TMIs are absent so that J(C) does not change under the variation of E-ex. On the contrary, in the case of the PMN-PT crystal with high Sa (on the order of a few hundreds of nm) E-ex induces TMIs in the Nb nanolayers. Specifically, the number of TMIs exhibits a non-monotonic increase on E-ex, thus causing a non-monotonic degradation of J(C). These experimental data are interpreted in terms of the variation of both volume strain and surface roughness on E-ex. This work highlights practical means to control the current-carrying capability of SC nanolayers through strain provided by PE substrates. (C) 2017 Elsevier B.V. All rights reserved.
River deltas sustain important ecosystems with rich biodiversity and large biomass, as well as human populations via the availability of water and food sources. Anthropogenic activities, such as urbanization, tourism and agriculture, may pose threats to river deltas. The knowledge of the factors controlling the regional water quality regime in these areas is important for planning sustainable use and management of the water resources. Here, hydrochemical methods and multivariate statistical techniques were combined to investigate the shallow aquifer of the Pinios River (Thessaly) deltaic plain with respect to water quality, hydrogeochemical evolution and interactions between groundwater and surface water bodies. Water quality assessment indicated that most of the river and groundwater samples fully comply with the criteria set by the Drinking Water Directive (98/83/EC). The river is recharged mainly from springs of the Tempi valley and the shallow aquifer, and to a lesser degree from precipitation, throughout the year. The hydrogeochemical characteristics indicated a cation (Ca, Mg, and Na) bicarbonate water type, which evolves to calcium-chloride, sodium-bicarbonate and sodium-chloride water type, in the northern part of the delta. Calcite and dolomite dissolution determined the major ion chemistry, but other processes, such as silicate weathering and cation exchange reactions, also contributed. In the northern part of the plain, the interaction with the deeper aquifer enriched the shallow aquifer with Na and Cl ions. Principal Component Analysis showed that five components (PCs) explain 77% of the total variance of water quality parameters; these are: (1) salinity; (2) water-silicate rocks interaction; (3) hardness due to calcite dissolution, and cation exchange processes; (4) nitrogen pollution; and (5) non-N-related artificial fertilizers. This study demonstrated that the variation of water hydrochemistry in the deltaic plain could be attributed to natural and anthropogenic processes. The interpretation of the PCA results dictated the parameters used for the development of a modified Water Quality Index (WQI), to provide a more comprehensive spatial representation of the water quality of the river delta.
Swift J1858.6-0814: Localization and variability of the optical counterpart G. Vasilopoulos (Yale), C. Bailyn (Yale), J. Milburn (Caltech) Following the detection of the new galactic transient source Swift J1858.6-0814 (Krimm et al. ATEL #12151) we performed photometric follow up observations of its optical counterpart using the WASP instrument on the Hale 200" Telescope at Palomar Observatory.
Historic data and old topographic maps include information on historical floods and paleo-floods. This paper aims at identifying the flood hazard by using historic data in the drainage basin of Pinios (Peneus) River, in Thessaly, central Greece. For this purpose, a catalogue of historical flood events that occurred between 1979 and 2010 and old topographic maps of 1881 were used. Moreover, geomorphic parameters such as elevation, slope, aspect and slope curvature were taken into account. The data were combined with the Geographical Information System to analyze the temporal and spatial distribution of flood events. The results show that a total number of 146 flood events were recorded in the study area. The number of flood events reaches its maximum value in the year 1994, while October contains the most flood events. The flood occurrences increased during the period 1990-2010. The flooded area reaches its maximum value in the year 1987, and November is the month with the most records. The type of damages with the most records is for rural land use. Regarding the class of damages, no human casualties were recorded during the studied period. The annual and monthly distribution of the very high category reaches the maximum values, respectively, in the year 2005 and in June. The analysis of the spatial distribution of the floods proves that most of the occurrences are recorded in the southern part of the study area. There is a certain amount of clustering of flood events in the areas of former marshes and lakes along with the lowest and flattest parts of the study area. These areas are located in the central, southern, south-eastern and coastal part of the study area and create favorable conditions for flooding. The proposed method estimates the localization of sites prone to flood, and it may be used for flood hazard assessment mapping and for flood risk management.
BACKGROUND: The rate of transmitted drug resistance (TDR) may increase with wider use of antiretroviral therapy and can contribute to therapeutic failure. We analysed time trends in TDR among HIV seroconverters.
METHODS: Using CASCADE data of individuals with well estimated dates of HIV seroconversion, we examined HIV nucleotide sequences collected prior to antiretroviral therapy use from 1996-2012. All samples were taken within 12 months of testing HIV positive. Using logistic regression, we examined the association between TDR and year of seroconversion, adjusting for confounders.
RESULTS: Of 4717 individuals seroconverting between 1996 and 2012, median (IQR) age at seroconversion was 33 (27, 39) years. The majority (3839; 92%) were male, mainly exposed through MSM (3767; 80%), and infected with subtype B (3464; 73%). Overall, 515 (11%) individuals had at least one drug resistance-related mutation; 280 individuals with nucleoside reverse transcriptase, 185 with nonnucleoside reverse transcriptase, and 144 with protease inhibitor mutations. Estimated TDR prevalence was 19.4% (8.2, 36.0) in 1996, significantly decreasing to 8.5% (5.9, 11.9) in 2012 [odds ratio (OR; 95% confidence interval (CI)) = 0.92 (0.90, 0.95) per year increase]. Individuals exposed through sex between men and women were significantly less likely to have been infected with a drug-resistant strain [OR (95% CI) = 0.59 (0.41, 0.87) compared with MSM], and there was marginal evidence that sampling during acute infection was associated with higher odds of resistance [OR (95% CI) = 1.20 (0.97, 1.7), P = 0.093] compared with later sampling.
CONCLUSION: TDR has decreased over calendar time although a significant proportion of new infections still carry resistance-related mutations.
The purpose of this paper is to examine the impact of terrorism on tourism demand in Greece using monthly data from 1977 to 2012. We investigate whether this relationship is bidirectional and whether it exhibits long run persistence. Thus, we employ a large dataset of terrorist incidents and perform cointegration and long-run causality tests, correcting our data for cyclical seasonality and applying PCA to construct a terrorism proxy according to the severity of the incident. Our findings concur that terrorism has a significant negative impact on tourist arrivals to Greece and that causality is noted from terrorism to tourism only. The results suggest that authorities should establish firm measures against terrorism and that further actions should be taken to promote tourism, safety and security, as a response to terrorist incidents. Our study is, to the best our knowledge, the first to approach terrorism using a three-factor proxy with qualitative features.
This study documented the change in study design and statistics employed in applied sports and exercise biomechanics research from 1985 to 2014. The sample comprised 676 data based original research reports published in the Journal of Applied Biomechanics (JAB) from 1985 to 2014. Eight design and 10 statistical criteria were extracted from each study. Descriptive statistics were calculated and change in study criteria over time were documented. Design criteria that did not change over time, remaining at relatively low levels of rigor, were widespread (71%) use of small (2-20) sample sizes and examination of numerous dependent variables (26.6% with >13). The number of experimental groups and independent variables also did not change with typically 1 to 2 reported. There was a significant 61% linear increase in randomization of participants into groups, however by 2014 still a minority (39%) of studies were not reporting randomized assignment. Types of statistical analysis showed positive changes over time with a 48% quadratic decrease in descriptive analyses, a 3% linear increase in nonparametric statistics, and a 45% linear increase in reporting parametric statistical analysis. Changes in specific statistical methods included a 9% linear decrease in bivariate correlation and a 73% linear increase in ANOVA. Reporting of assumptions had a 35% linear increase, yet in 2014 sixty-five percent still did not report on meeting statistical assumptions. Changes in test statistics included a linear 56% increase of reporting observed P values and a quadratic 29% increase in reporting effect sizes beginning in the late 1990s. It was concluded there was evidence of small improvements in research design and statistics in JAB over the last 30 years; however, there is still room for improvement to meet higher levels of research rigor and current recommendations on statistical analysis and reporting.
OBJECTIVES: This study aimed to investigate size and shape variation of human premolars between Indigenous Australians and Australians of European ancestry, and to assess whether sex and ancestry could be differentiated between these groups using 3D geometric morphometrics.
MATERIALS AND METHODS: Seventy dental casts from each group, equally subdivided by sex, were scanned using a structured-light scanner. The 3D meshes of upper and lower premolars were processed using geometric morphometric methods. Seventy-two landmarks were recorded for upper premolars and 50 landmarks for lower premolars. For each tooth type, two-way ANOVA was used to assess group differences in centroid size. Shape variations were explored using principal component analysis and visualized using 3D morphing. Two-way Procrustes ANOVA was applied to test group differences for ancestry and sex, and a "leave-one-out" discriminant function was applied to assess group assignment.
RESULTS: Centroid size and shape did not display significant difference between the sexes. Centroid size was larger in Indigenous Australians for upper premolars and lower second premolars compared to the Australians of European ancestry. Significant shape variation was noted between the two ancestral groups for upper premolars and the lower first premolar. Correct group assignment of individual teeth to their ancestral groups ranged between 80.0 and 92.8% for upper premolars and 60.0 and 75.7% for lower premolars.
DISCUSSION: Our findings provide evidence of significant size and shape variation in human premolars between the two ancestral groups. High classification rates based on shape analysis of upper premolars highlight potential application of geometric morphometrics in anthropological, bioarcheological and forensic contexts.
Magnetically doped topological insulators may produce novel states of electronic matter, where for instance the quantum anomalous Hall effect state can be realized. Pivotal to this goal is a microscopic control over the magnetic state, defined by the local electronic structure of the dopants and their interactions. We report on the magnetic coupling among Mn or Co atoms adsorbed on the surface of the topological insulator Bi2Te3. Our findings uncover the mechanisms of the exchange coupling between magnetic atoms coupled to the topological surface state in strong topological insulators. The combination of x-ray magnetic circular dichroism and ab initio calculations reveals that the sign of the magnetic coupling at short adatom-adatom distances is opposite for Mn with respect to Co. For both elements, the magnetic exchange reverses its sign at a critical distance between magnetic adatoms, as a result of the interplay between superexchange, double exchange and Ruderman-Kittel-Kasuya-Yoshida interactions.
A novel, fit-for-purpose, highly sensitive, analytical UPLC-PDA methodology was developed and fully validated, according to ICH, FDA and EMA guidelines, for the rapid and accurate quantification of trans-crocin 4 (TC4) and crocetin (CRC) in mice plasma and brain after i.p. administration. A PDA based methodology shows a wider applicability as it is cost effective and can be easily and seamlessly adopted by the pharma industry. The separation of the analytes was performed on a C18 Hypersil Gold column with 2.5 min run time, employing the internal standard (ISTD) methodology. The two methods were successfully applied for the determination of CRC and TC4 in mouse plasma and brain after i.p. administration of TC4 (50 mg/kg) in a time range of 0-240 min. Due to the selection of i.p. administration route, the first-pass metabolism and/or gastric hydrolysis were bypassed, a fact that enhanced the bioavailability of TC4. Furthermore, TC4 was found to be capable of crossing the Blood Brain Barrier (BBB) and build up levels in the mouse brain, regardless of its highly hydrophilic character. CRC was not detected in any plasma or brain sample, although it has been reported that TC4 quickly hydrolyzes to CRC after p.o. administration. Therefore i.p. administration could be used in the case of TC4 for the accurate determination of its biological role. Overall, the developed methodology offers important information about the bioavailability of TC4 in mouse plasma and for the first time, demonstrates the ability of TC4 to penetrate the BBB and localize inside the brain.
A novel, fit-for-purpose, highly sensitive, analytical UPLC-PDA methodology was developed and fully validated, according to ICH, FDA and EMA guidelines, for the rapid and accurate quantification of trans-crocin 4 (TC4) and crocetin (CRC) in mice plasma and brain after i.p. administration. A PDA based methodology shows a wider applicability as it is cost effective and can be easily and seamlessly adopted by the pharma industry. The separation of the analytes was performed on a C18 Hypersil Gold column with 2.5 min run time, employing the internal standard (ISTD) methodology. The two methods were successfully applied for the determination of CRC and TC4 in mouse plasma and brain after i.p. administration of TC4 (50 mg/kg) in a time range of 0-240 min. Due to the selection of i.p. administration route, the first-pass metabolism and/or gastric hydrolysis were bypassed, a fact that enhanced the bioavailability of TC4. Furthermore, TC4 was found to be capable of crossing the Blood Brain Barrier (BBB) and build up levels in the mouse brain, regardless of its highly hydrophilic character. CRC was not detected in any plasma or brain sample, although it has been reported that TC4 quickly hydrolyzes to CRC after p.o. administration. Therefore i.p. administration could be used in the case of TC4 for the accurate determination of its biological role. Overall, the developed methodology offers important information about the bioavailability of TC4 in mouse plasma and for the first time, demonstrates the ability of TC4 to penetrate the BBB and localize inside the brain.
The reaction of the syn-bimetallic bis(pentalene)dititanium complex Ti2(μ:η5,η5-Pn†)2 (Pn† = C8H4(1,4-SiiPr3)2) 1 with carbon suboxide (OCCCO, C3O2) results in trimerisation of the latter and formation of the structurally characterised complex [{Ti2(μ:η5,η5-Pn†)2}{μ-C9O6}]. The trimeric bridging C9O6 unit in the latter contains a 4-pyrone core, a key feature of both the hexamer and octamer of carbon suboxide which are formed in the body from trace amounts of C3O2 and are, for example, potent inhibitors of Na+/K+-ATP-ase. The mechanism of this reaction has been studied in detail by DFT computational studies, which also suggest that the reaction proceeds via the initial formation of a mono-adduct of 1 with C3O2. Indeed, the carefully controlled reaction of 1 with C3O2 affords [Ti2(μ:η5,η5-Pn†)2 (η2-C3O2)], as the first structurally authenticated complex of carbon suboxide.
Lots have happened in the East-Mediterranean since the beginning of the third millennium. A list of the main events reveals a long series of issues that took place in the region and heavily affect it. The impact of these issues and events will stay with us for the foreseeable future and is heavily discernible in the relations between the eastern- Mediterranean non- Arab littoral countries: Turkey, Israel, Greece and Cyprus. This list includes, for example, the 2000s deteriorating relations between Turkey and Israel (in 2016 the two resumed full diplomatic relations); the current crises in the relations between Turkey several Middle Eastern countries (Egypt, Syria, Iraq); the civil war that rages in Syria since 2011 and Turkey’s deep involvement in it; acts of Islamist led terrorism, attacks by ISIS1, and manifestations of religious extremism that heavily affect daily life, economies and tourism in Turkey, Egypt, Jordan, Iraq, as well as in other places; illegal immigrants, refugees and asylum seekers that inundate Turkey, Greece (and via them) many EU countries; energy explorations in the Mediterranean Sea; issues related to civil rights, minorities, the growing tendencies towards strong executives and authoritarian regimes at the expense of democracy and pluralism; heavy internal changes like the decline of Turkish Kemalism, the growing impact of religion in Israel and Turkey, and the fi nancial crisis of Greece; In addition, the struggle for self-determination of various ethnic groups and peoples (the Kurds, the Palestinians, and others); the Russian reappearance, American disappearance and the EU weak impact on events in the Middle East and the Eastern – Mediterranean - - these and subjects have brought about dramatic changes in the Eastern Mediterranean sub-system. 1. See at the end of this text: ANNEX: Evidence on the Collaboration of Turkey with ISIS and other jihadist groups, as well as relevant testimonies from international sources. From the Turkish standpoint, the events in our region express what is happening in the global arena: Europe is aging, its economy and currency are limping and convulsing, and the EU radiates political disintegration (the Brexit) and economic weaknesses. The US is suffering from an economic crisis, it is globally and politically on the decline, internally it is also on the decline following the eight years’ presidency of Barak Obama, and the November 2016 election of President Donald Trump. Simultaneously, other powers (China, Russia) strive to replace American hegemony all around the globe. Also from the standpoint of Ankara, Turkey desires to be now a rising «uneasy» power, and is demarcating the limits of its infl uence: Central Asia, the Caucasus and the Balkans, on the one hand; black Africa, North Africa, and the Middle East, on the other. Following several setbacks, Turkish- Russian relations are developing and disagreements have been settled. And all these developments and events have greatly affected the three eastern - Mediterranean non - Muslim countries (Greece, Israel, Cyprus), and the region’s sole non- Arab but Muslim country- Turkey. In our paper we will make an attempt to describe and analyze the major impacts that these events and issues have had on the developments in the eastern- Mediterranean, with particular focus on Turkey and Israel, and the growing cooperation between Greece and Israel.
The discovery of Weyl semimetals represents a significant advance in topological band theory. They paradigmatically enlarged the classification of topological materials to gapless systems while simultaneously providing experimental evidence for the long-sought Weyl fermions. Beyond fundamental relevance, their high mobility, strong magnetoresistance, and the possible existence of even more exotic effects, such as the chiral anomaly, make Weyl semimetals a promising platform to develop radically new technology. Fully exploiting their potential requires going beyond the mere identification of materials and calls for a detailed characterization of their functional response, which is severely complicated by the coexistence of surface-and bulk-derived topologically protected quasiparticles, i.e., Fermi arcs and Weyl points, respectively. Here, we focus on the type-II Weyl semimetal class in which we find a stoichiometry-dependent phase transition from a trivial to a nontrivial regime. By exploring the two extreme cases of the phase diagram, we demonstrate the existence of a universal response of both surface and bulk states to perturbations. We show that quasiparticle interference patterns originate from scattering events among surface arcs. Analysis reveals that topologically nontrivial contributions are strongly suppressed by spin texture. We also show that scattering at localized impurities can generate defect-induced quasiparticles sitting close to the Weyl point energy. These give rise to strong peaks in the local density of states, which lift the Weyl node, significantly altering the pristine low-energy spectrum. Remarkably, by comparing the WTe2 and the MoTe2 cases we found that scattering response and topological transition are not directly linked. Visualizing the existence of a universal microscopic response to scattering has important consequences for understanding the unusual transport properties of this class of materials. Overall, our observations provide a unifying picture of the type-II Weyl phase diagram.
Hepatitis B virus (HBV) infection constitutes a global public health problem. In order to establish how HBV was disseminated across different geographic regions, we estimated the levels of regional clustering for genotypes D and A. We used 916 HBV-D and 493 HBV-A full-length sequences to reconstruct their global phylogeny. Phylogeographic analysis was conducted by the reconstruction of ancestral states using the criterion of parsimony. The putative origin of genotype D was in North Africa/Middle East. HBV-D sequences form low levels of regional clustering for the Middle East and Southern Europe. In contrast, HBV-A sequences form two major clusters, the first including sequences mostly from sub-Saharan Africa, and the second including sequences mostly from Western and Central Europe. Conclusion: We observed considerable differences in the global dissemination patterns of HBV-D and HBV-A and different levels of monophyletic clustering in relation to the regions of prevalence of each genotype.
{The purpose of the present study was to determine whether a battery of anthropometric and lower and upper body strength, speed and power tests predicted selection of young volleyball players for a Junior National Team by expert coaches. Fifty-two male junior volleyball players (14.8±0.5 yrs, height: 1.84±0.05 m, body mass: 72.5±7.1 kg) took part in a training camp and underwent a selection procedure by expert coaches' of the junior national team. Anthropometric data and fitness tests results were obtained and players were graded on a scale from 0 to 100 on the basis of their performance in a volleyball tournament. Selected players were superior in the majority of measured variables (p≤0.017) and had higher grading scores compared with non-selected players (85.3±4.1 vs. 70.5±5.6, respectively, p<0.01). The combination of spike jump and reach test (SJR) and 3 kg medicine ball throw velocity (MB3) explained 63.5% of the variance in expert coaches' grading (p<0.001). A multivariate discriminant analysis yielded a significant discriminant function (Wilk's lambda= 0.55, χ= 29.324, p<0.001, η=0.82). SJR and MB3 were the only variables that contributed to the discriminant function (standardized function coefficients: SJR = 0.68
Principal component analysis (PCA) is being extensively used in Astronomy but not yet exhaustively exploited for variability search. The aim of this work is to investigate the effectiveness of using the PCA as a method to search for variable stars in large photometric data sets. We apply PCA to variability indices computed for light curves of 18 152 stars in three fields in M 31 extracted from the Hubble Source Catalogue. The projection of the data into the principal components is used as a stellar variability detection and classification tool, capable of distinguishing between RR Lyrae stars, long-period variables (LPVs) and non-variables. This projection recovered more than 90 per cent of the known variables and revealed 38 previously unknown variable stars (about 30 per cent more), all LPVs except for one object of uncertain variability type. We conclude that this methodology can indeed successfully identify candidate variable stars.
We have determined the membership of 46 open clusters. For the nine clusters within 250pc we determined optimised parallaxes based on the combined information extracted from the measured parallax and proper motion values. These clusters are : in Tables A1a & A3: alphaPer, Blanco1, ComaBer, Hyades, IC2391, IC2602, NGC2451A, Pleiades, Praesepe. The remaining 37 clusters are in Table A1b & A4: Coll140, IC4651, IC4665, IC4725, IC4756, NGC0188, NGC0752, NGC0869, NGC0884, NGC1039, NGC1901, NGC2158, NGC2168, NGC2232, NGC2323, NGC2360, NGC2422, NGC2423, NGC2437, NGC2447, NGC2516, NGC2547, NGC2548, NGC2682, NGC3228, NGC3532, NGC6025, NGC6281, NGC6405, NGC6475, NGC6633, NGC6774, NGC6793, NGC7092, Stock2, Trump02, Trump10. (4 data files).
Our analysis was performed on the iDR4 consisting of ~260000 single exposures (corresponding to ~100 000 stacked spectra) of about 51000 distinct stars observed with the FLAMES instrument feeding the optical spectrographs GIRAFFE (with setups HR3, HR5A, HR6, HR9B, HR10, HR14A, HR15N, HR15, HR21) and UVES (with setups U520 and U580) covering the optical and near-IR wavelength ranges. (5 data files).
We used archival observations of NGC 4535 taken with the HST WFPC2 as part of the HST Key Project. We used 13 epochs of F555W (equivalent to Johnson V) and 9 epochs of F814W (equivalent to Kron-Cousins I). The F555W data consisted in total of eight 3x1200 sec exposures and five 4x1300s exposures, while the F814W of nine 3x1300s exposures. (1 data file).
We have observed two fields in the northern disc of M31 in a large programme (LP) of XMM-Newton (PI: M. Sasaki). The data were taken with the European Photon Imaging Cameras (EPICs) in full-frame mode using the thin filter for the pn camera (EPIC-pn) and the medium filter for the two MOS cameras (EPIC-MOS1/2), in order to minimise the contamination by background but to maximise the sensitivity for soft diffuse emission. Each of the two fields was observed twice. (6 data files).
Plant species grown in the Mediterranean region have been selected for an experimental study regarding plant ability to endure harsh, abiotic conditions such as water deficiency. Young plants of Ceratonia siliqua L., Myrtus communis L., Nerium oleander L., Rosmarinus officinalis L. and Pittosporum tobira (Thunb.) were placed in elongated plastic tubes and rhizotrons, which allow non-destructive observations of plant material, and were grown under controlled light and temperature conditions, with varying irrigation patterns in a growth chamber, for a 30-day period. Sugar, proline and chlorophyll content were measured in above and below ground tissues of the considered species, during the 30-day experimental period. The results show that Ceratonia siliqua, Myrtus communis and Nerium oleander are more capable of enduring drought conditions, in comparison to Rosmarinus officinalis L. and Pittosporum tobira (Thunb.). The results can be used for planning green urban landscapes with introduction of naturalistic planting, using plant species adapted to the scarcity of water during prolonged, ambient drought conditions.
This paper examines Greek invitations as first pair parts of an adjacency pair within the framework of Conversation Analysis. The focus of our research is on impromptu invitations issued via polar negative-interrogative constructions and the effects this type of con- struction has on the interactional organization of the whole invitation-response sequence. It is suggested that the issuing of the invitation by means of this particular syntactic choice has ramifications for the unfolding of the interaction with respect to sequencing and preference organization. Specifically, it is maintained that impromptu invitations issued by means of polar negative-interrogatives in Greek tend to trigger markedly delayed accep- tances. It is suggested that the explanation for this phenomenon lies in the “cross-cutting” preferences built in the construction when used to make impromptu invitations.
This paper examines Greek invitations as first pair parts of an adjacency pair within the framework of Conversation Analysis. The focus of our research is on impromptu invitations issued via polar negative-interrogative constructions and the effects this type of construction has on the interactional organization of the whole invitation-response sequence. It is suggested that the issuing of the invitation by means of this particular syntactic choice has ramifications for the unfolding of the interaction with respect to sequencing and preference organization. Specifically, it is maintained that impromptu invitations issued by means of polar negative-interrogatives in Greek tend to trigger markedly delayed acceptances. It is suggested that the explanation for this phenomenon lies in the “cross-cutting” preferences built in the construction when used to make impromptu invitations.
PSR J2032+4127 is a young and rapidly rotating pulsar on a highly eccentric orbit around the high-mass Be star MT91 213. X-ray monitoring of the binary system over an ∼4000 d period with Swift has revealed an increase of the X-ray luminosity which we attribute to the synchrotron emission of the shocked pulsar wind. We use Swift X-ray observations to infer a clumpy stellar wind with r-2 density profile and constrain the Lorentz factor of the pulsar wind to 105 < γw < 106. We investigate the effects of an axisymmetric stellar wind with polar gradient on the X-ray emission. Comparison of the X-ray light curve hundreds of days before and after the periastron can be used to explore the polar structure of the wind.
ATel #12209 reported the detection of a new X-ray transient in the Small Magellanic Cloud (SMC), Swift J005139.2-721704, exhibiting outbursting activity. The system has been classified as a new SMC high-mass X-ray binary based on its identification with the B-type star [M2002]20671.
This paper presents a brief literature review on the impact of Waldorf education on students’ and graduates’ socio-emotional health. The main elements of Waldorf education which contribute to socio-emotional health are identified and described, namely, integration of the arts, movement and play, promoting relationships, reconnecting with nature, and spiritual development. Finally, the positive impact of Waldorf education on the reduction of attention deficit hyperactivity disorder is analyzed.
Cytosolic phospholipase A2 (GIVA cPLA2) is the only PLA2 that exhibits a marked preference for hydrolysis of arachidonic acid containing phospholipid substrates releasing free arachidonic acid and lysophospholipids and giving rise to the generation of diverse lipid mediators involved in inflammatory conditions. Thus, the development of potent and selective GIVA cPLA2 inhibitors is of great importance. We have developed a novel class of such inhibitors based on the 2-oxoester functionality. This functionality in combination with a long aliphatic chain or a chain carrying an appropriate aromatic system, such as the biphenyl system, and a free carboxyl group leads to highly potent and selective GIVA cPLA2 inhibitors (XI(50) values 0.00007–0.00008) and docking studies aid in understanding this selectivity. A methyl 2-oxoester, with a short chain carrying a naphthalene ring, was found to preferentially inhibit the other major intracellular PLA2, the calcium-independent PLA2. In RAW264.7 macrophages, treatment with the most potent 2-oxoester GIVA cPLA2 inhibitor resulted in over 50% decrease in KLA-elicited prostaglandin D2 production. The novel, highly potent and selective GIVA cPLA2 inhibitors provide excellent tools for the study of the role of the enzyme and could contribute to the development of novel therapeutic agents for the treatment of inflammatory diseases.
INTRODUCTION: Aberrant subvesical bile ducts are a scarce anatomical variation, consisted by a network of bile ducts located in the peri-hepatic capsule of the gallbladder fossa. These rare ducts are usually discovered intraoperatively and their presence poses the risk of bile injury and clinically significant bile leak.
PRESENTATION OF CASE: Aberrant subvesical bile ducts were unexpectedly identified in a young woman during laparoscopic cholecystectomy. These three ducts were clipped carefully for avoidance of bile duct injury and subsequent bile leak. The operation was uneventful. A meticulous review of the recent literature was conducted as well.
DISCUSSION: This unusual anatomical variation of the biliary tract is mainly discovered during the operation. Thus, surgical injury of these ducts is nearly inevitable and it provokes the severe complication of bile leak. Bile injury represents the most crucial and life-threatening postoperative complication of cholecystectomies. Surgeons in the right upper quadrant of the abdomen should be constantly aware of this rare anatomical variation.
CONCLUSION: Aberrant subvesical bile ducts are associated with a high risk of surgical bile duct injury. Nevertheless, meticulous operative technique combined with surgeons' perpetual awareness concerning this peculiar anatomical aberration leads to a safe laparoscopic cholecystectomy.
Synetos A, Toutouzas K, Benetos G, Drakopoulou M, Stathogianis K, Latsios G, Agrogiannis G, Papalois A, Anagnostopoulos K, Cokkinos D, et al.Abstract 17995. Circulation. 2017;136.
Accreditation is an important process ensuring quality of academic programs in Biomedical Informatics. Certification ensures standards in knowledge and skills of professionals in Health Informatics. The process of Accreditation is analyzed for its pros and cons. Procedures are applied to ensure the completion of accreditation of a program is transparent, objective and leads in quality qualifications. Finally, the logistics required for Accreditation are calculated in terms of human resources as well to meeting cost expectations.
Moschos MM, Chatziralli I, Gazouli M, Brouzas D, Cennamo G, Eidenberger T, Forte R, Panzella L, Cesarano I, Napolitano A, et al.Acknowledgements to the Referees. Ophthalmic Research. 2017;58:243–243.
Using an extension of the full elastodynamic layer-multiple-scattering method to structures of fluid-saturated poroelastic spherical bodies, a comprehensive theoretical study of the acoustic response of double-porosity submerged liquid-saturated granular polymeric materials of specific morphology consisting of touching porous polymer spheres arranged in a fcc lattice, beyond the long-wavelength effective-medium description, is presented. Calculated transmission and absorption spectra of finite slabs of these materials are analyzed by reference to the acoustic modes of the constituent porous spherical grains as well as to relevant dispersion diagrams of corresponding infinite crystals, and a consistent interpretation of the results is provided. In particular, it is shown that resonant modes with very long lifetime, localized in the spheres in the form of slow longitudinal waves, which are peculiar to poroelastic materials, are formed when the viscous length is much shorter than the radius of the inner pores of the spheres. These modes, which can be easily tuned in frequency by adjusting the intrinsic porosity of the spheres, induce some remarkable features in the acoustic behavior of these double-porosity materials, such as narrow dispersionless absorption bands and directional transmission gaps.
Karampatsos, GP, Korfiatis, PG, Zaras, ND, Georgiadis, GV, and Terzis, GD. Acute effect of countermovement jumping on throwing performance in track and field athletes during competition. J Strength Cond Res 32(1): 359-364, 2017-The purpose of the study was to investigate whether performing 3 consecutive countermovement jumps (CMJs) just before an attempt enhances performance in track and field throwers during competition. Twelve shot putters, 8 hammer throwers, 9 discus throwers, and 3 javelin throwers of both sexes participated in the study. They performed 3 maximal CMJs 85 ± 12 seconds before the second, fourth, and sixth attempt during 3 different official competitions of national level. Maximal strength (1 repetition maximum [1RM]) in squat and bench press was measured 1 week after the competition. Mean throwing performance was significantly higher after the CMJs intervention (2.66 ± 4.3%, range of increase 0.02-18.98%, p = 0.0001). Similarly, maximum throwing performance was significantly higher after the CMJs (2.76 ± 3.29%, range of increase 0.09-13.93%, p = 0.0009). All but 2 athletes increased their best performance after the CMJs. The percentage increase in performance was similar between sexes (male athletes 2.56 ± 3.01%; female athletes 3.06 ± 3.76%, p = 0.677), but it was higher for the "lighter throws" (discus and javelin throw: 4.66 ± 4.11%) compared with the "heavier throws" (shot and hammer throw: 1.62 ± 2.04%, p = 0.008). The percentage increase in performance was not significantly correlated with 1RM squat or bench press, anthropometric characteristics, and personal best performance. These results suggest that performing 3 CMJs approximately 1 minute before an attempt may increase track and field throwing performance during competition.
Research on students’ motivation has mainly focused on interpersonal differences rather than on the ongoing, intrapersonal dynamics that forge students’ everyday life. In this five-month longitudinal (diary) study, we recruited a sample of 179 high school students from Greece (35.8% males; Mage = 16.27; SD = 1.02) to investigate through multilevel analyses the ongoing dynamics of students’ motivation. We did so by examining the relation between autonomous functioning and aspects of study regulation (namely, study efforts and procrastination) and well-being (namely, subjective vitality and depressive feelings). After controlling for perceived competence, we found week-to-week autonomous functioning to relate positively to study efforts and subjective vitality and negatively to procrastination and depressive feelings. Interestingly, implicit theories of ability - the degree to which one believes that ability is fixed or amenable - were found to moderate the week-to-week relations of autonomous functioning to study efforts and homework procrastination. In particular, autonomous functioning co-varied positively to study efforts and negatively to homework procrastination only among students who believed that ability is malleable. Also, beliefs that ability is fixed predicted poorer grades, lower mean levels of study efforts, and higher homework procrastination. The results underscore the necessity of taking a more dynamic view when studying motivational phenomena and the importance of jointly considering the implicit theory framework and self-determination theory.
We look at Poisson geometry taking the viewpoint of singular foliations, understood as suitable submodules generated by Hamiltonian vector fields rather than partitions into (symplectic) leaves. The class of Poisson structures which behave best from this point of view, are those whose submodule generated by Hamiltonian vector fields arises from a smooth holonomy groupoid. We call them almost regular Poisson structures and determine them completely. They include regular Poisson and log symplectic manifolds, as well as several other Poisson structures whose symplectic foliation presents singularities. We show that the holonomy groupoid associated with an almost regular Poisson structure is a Poisson groupoid, integrating a naturally associated Lie bialgebroid. The Poisson structure on the holonomy groupoid is regular, and as such it provides a desingularization. The holonomy groupoid is "minimal" among Lie groupoids which give rise to the submodule generated by Hamiltonian vector fields. This implies that, in the case of log-symplectic manifolds, the holonomy groupoid coincides with the symplectic groupoid constructed by Gualtieri and Li. Last, we focus on the integrability of almost regular Poisson manifolds and exhibit the role of the second homotopy group of the source-fibers of the holonomy groupoid.
Au sein du complexe géopolitique méditerranéen, on observe ces derniers mois des mouvements dangereux de grande ampleur, dans le sous-système des Balkans occidentaux. La Grèce a le funeste privilège de constituer la frontière des deux sous-systèmes du complexe géopolitique méditerranéen: a. du sous-système des Balkans et, b. du sous-système turco-proche-oriental. Nous allons démontrer d’une part, que ces deux sous-systèmes se trouvent confrontés à des disfonctionnements intenses et instables et d’autre part, que le facteur géopolitique déterminant de l’instabilité est celui de la sécurité énergétique. De surcroit, nous montrerons que les deux principaux pôles de puissances internationales que sont Moscou et Washington, de par leur antagonisme de type néo guerre froide, confèrent une dynamique déstabilisatrice au complexe géopolitique méditerranéen, dont l’épicentre se trouve dans les Balkans de l’ouest (Balkans occidentaux). La notion dite: «de type néo guerre froide» est utilisée pour souligner le fait qu’il existe une grande distance politico idéologique entre ces deux pôles (et bien sur, il en existe une plus grande encore entre Washington et Pékin), comme pendant la période de la guerre froide. La différence idéologique, en l’occurrence, réside entre l’approche cosmopolite et ultra libérale occidentale et l’approche nationale-identitaire conservatrice orientale, avec comme pole dominant Moscou. Le corpus théorique de la présente analyse est celui de la géopolitique anglo-saxonne classique, issue de l’école de N. Spykman, ayant évolué avec l’approche de Z. Brzezinsky et celui de l’analyse géopolitique systémique contemporaine
INTRODUCTION: Identification and preservation of the facial nerve (FN) is a major challenge when performing parotidectomy. Anatomic variations of the relation between the FN and the retromandibular vein (RMV) pose a high risk of nerve injury and bleeding during the operation.
PRESENTATION OF CASE: An unusual anatomic variation of the relation between the FN and the RMV was unexpectedly detected during superficial parotidectomy. The operation was uneventful. A meticulous review of the recent literature was conducted as well.
DISCUSSION: Variations of the relation between the FN and the RMV are mainly identified during the operation, since when performing parotidectomy, surgeons typically detect all the FN branches by locating the RMV. Such kind of variations, are not as rare as considered and their presence complicates parotid surgery and increases the potentiality of nerve injury and hemorrhage.
CONCLUSION: Surgeons' deep knowledge and perpetual awareness concerning the probable anatomic variations of the relation between the FN and the RMV combined with detailed exposure of the operative field and of the relationship between these adjacent anatomical structures lead to safe parotid surgery.
Complete mesocolic excision (CME) is a standardized surgical procedure for colonic cancer that requires ample knowledge of the anatomical patterns of the colic arteries. Variations of the colic vessels encumber both surgical and endovascular techniques. In the presented case below, the right colic artery was incidentally detected emerging from the right gastroepiploic artery, during CME. Surgeons should be always aware of this variation in order to perform safe abdominal surgeries and sufficient resection of the regional lymph nodes with a view to minimizing the probability of recurrence of disease when encountering colonic cancer.
In therapeutic interventions associated with melanin hyperpigmentation, tyrosinase is regarded as a target enzyme as it catalyzes the rate-limiting steps in mammalian melanogenesis. Since many known agents have been proven to be toxic, there has been increasing impetus to identify alternative tyrosinase inhibitors, especially from natural sources. In this study, we investigated 900 extracts from Greek plants for potential tyrosinase inhibitive properties. Among the five most potent extracts, the methanol extract of Morus alba wood (MAM) demonstrated a significant reduction in intracellular tyrosinase and melanin content in B16F10 melanoma cells. Bioassay-guided isolation led to the acquisition of twelve compounds: oxyresveratrol (1), kuwanon C (2), mulberroside A (3), resorcinol (4), dihydrooxyresveratol (5), trans-dihydromorin (6), 2,4,3′-trihydroxydihydrostilbene (7), kuwanon H (8), 2,4-dihydroxybenzaldehyde (9), morusin (10), moracin M (11) and kuwanon G (12). Among these, 2,4,3′-trihydroxydihydrostilbene (7) is isolated for the first time from Morus alba and constitutes a novel potent tyrosinase inhibitor (IC50 0.8 ± 0.15). We report here for the first time dihydrooxyresveratrol (5) as a potent natural tyrosinase inhibitor (IC50 0.3 ± 0.05). Computational docking analysis indicated the binding modes of six tyrosinase inhibitors with the aminoacids of the active centre of tyrosinase. Finally, we found both MAM extract and compounds 1, 6 and 7 to significantly suppress in vivo melanogenesis during zebrafish embryogenesis.
Filamentous fungi provide excellent systems for investigating the role of the AP-2 complex in polar growth. Using Aspergillus nidulans, we show that AP-2 has a clathrin-independent essential role in polarity maintenance and growth. This is in line with a sequence analysis showing that the AP-2 β subunit (β2) of higher fungi lacks a clathrin-binding domain, and experiments showing that AP-2 does not co-localize with clathrin. We provide genetic and cellular evidence that AP-2 interacts with endocytic markers SlaB(End4) and SagA(End3) and the lipid flippases DnfA and DnfB in the sub-apical collar region of hyphae. The role of AP-2 in the maintenance of proper apical membrane lipid and cell wall composition is further supported by its functional interaction with BasA (sphingolipid biosynthesis) and StoA (apical sterol-rich membrane domains), and its essentiality in polar deposition of chitin. Our findings support that the AP-2 complex of dikarya has acquired, in the course of evolution, a specialized clathrin-independent function necessary for fungal polar growth.
Eukaryotic cells heavily depend on ATP generated by oxidative phosphorylation (OXPHOS) within mitochondria. Besides being the main suppliers of cell's energy, mitochondria also provide an additional compartment for a wide range of cellular processes and metabolic pathways. Mitochondria constantly undergo fusion/fission events and form a mitochondrial network, which is a highly dynamic, tubular structure allowing for rapid and continuous exchange of genetic material, as well as, targeting dysfunctional mitochondria for degradation through mitochondrial selective autophagy (mitophagy). Mitophagy mediates the elimination of damaged and/or superfluous organelles, maintaining mitochondrial and cellular homeostasis. In this chapter, we present two versatile, noninvasive methods, developed for monitoring in vivo mitophagy in C. elegans. These procedures enable the assessment of mitophagy in several cell types during development or under stress conditions. Investigating the role of mitophagy at the organismal level is essential for the development of therapeutic interventions against age-related diseases.
PURPOSE: β-catenin and AXIN2 play an important role in the Wnt signaling pathway. The aim of this study was to investigate β-catenin and AXIN2 expression in colorectal cancer (CRC) and relate these findings with patients' clinicopathological features and prognosis.
METHODS: 57 consecutive patients with surgically treated CRC were included in this study. Quantitative PCR and immunohistochemistry (IHC) analyses were performed to characterize the expression of the aforementioned markers in CRC tissues.
RESULTS: β-catenin overexpression in the nucleus was associated with advanced N stage CRCs (p=0.04). Multivariate Cox regression analysis showed that β-catenin overexpression is an independent prognostic factor for overall survival (OS). A positive correlation between β-catenin location and AXIN2 mRNA was observed.
CONCLUSIONS: Nuclear β-catenin is a valuable prognostic factor. AXIN2 is a component of the "Destruction Complex" and also a Wnt target gene. However, the clinical importance of AXIN2 expression in CRC remains unclear.
The thermal comfort conditions in a complex urban area is influenced by the surrounding structures and obstacles which modify the incoming radiation fluxes. A measure of this modification is the sky view factor (SVF), which could be estimated in each point of a selected area if a high resolution digital elevation model (DEM), or other urban morphological data including the manmade infrastructure, are available. The goal of this study is to model the continuous SVF for a complex building environment in the campus of National and Kapodistrian University of Athens, based on a high resolution DEM (0.09 m). For this purpose, we applied the structure-from-motion (SfM) technique, which takes advantage of the interpretation of ultra-high resolution colour images acquired by remotely piloted airborne systems, also known as drones or unmanned aerial vehicles. A quantitative analysis, by applying statistical metrics, yields perfect agreement between modelled and observed SVF values, over the examined area. The proposed methodology could be applied for human-biometeorology research in micro scale complex urban environments.
The thermal comfort conditions in a complex urban area is influenced by the surrounding structures and obstacles which modify the incoming radiation fluxes. A measure of this modification is the sky view factor (SVF), which could be estimated in each point of a selected area if a high resolution digital elevation model (DEM), or other urban morphological data including the manmade infrastructure, are available. The goal of this study is to model the continuous SVF for a complex building environment in the campus of National and Kapodistrian University of Athens, based on a high resolution DEM (0.09 m). For this purpose, we applied the structure-from-motion (SfM) technique, which takes advantage of the interpretation of ultra-high resolution colour images acquired by remotely piloted airborne systems, also known as drones or unmanned aerial vehicles. A quantitative analysis, by applying statistical metrics, yields perfect agreement between modelled and observed SVF values, over the examined area. The proposed methodology could be applied for human-biometeorology research in micro scale complex urban environments.
Serial 3-dimensional dental model superimposition provides a risk-free, detailed evaluation of morphological alterations on a patient's mouth. Here, we evaluated accuracy and precision of five palatal areas, used for superimposition of maxillary 3D digital dental casts. Sixteen pre- and post-orthodontic treatment dental casts of growing patients (median time lapse: 15.1 months) were superimposed on each palatal area using the iterative closest point algorithm. Area A (medial 2/3 of the third rugae and a small area dorsal to them) was considered the gold standard, due to high anatomical stability. Areas B, C, and D added a distal extension along the midpalatal raphe, an anterior extension to the second rugae, and the remaining palatal surface, respectively. Area E was similar to A, located more posteriorly. Non parametric multivariate models showed minimal or no effect on accuracy and precision by operator, time point, or software settings. However, the choice of superimposition area resulted in statistically significant differences in accuracy and clinically significant differences in detected tooth movement (95% limits of agreement exceeding 1 mm and 3°). Superimposition on area A provided accurate, reproducible, and precise results. Outcomes were comparable for area B, but deteriorated when alternative areas were used.
The recent global financial crisis has triggered questions in the scientific area of capital structure dynamic determination regarding how “quickly” companies tend to adjust their capital structure to their long-term targets, in different macroeconomic states. We broaden the scope of the debate by focusing on SMEs and by discussing the relative importance of firm-specific and macroeconomic variables, when macroeconomic conditions change. Based on a partial adjustment model, we find that short-term and long-term debt ratios follow different patterns regarding their adjustment speeds; the adjustment speed for long-term debt slows down during the crisis, while the respective of the short-term debt is not affected. We also find clear differentiations of the effects and the contribution of the firm-specific and the macroeconomic variables between short-term debt and long-term debt ratios, when macroeconomic states change. We thus conclude that the nature and maturity of borrowing affect the persistence and endurance of the relationship between determinants and borrowing, across different macroeconomic states.
The magneto-optical response of a Faraday-active Fabry–Pérot etalon with birefringent mirrors is studied by means of electrodynamic simulations using the finite-element and the scattering-matrix methods. The specific structure under consideration consists of a magnetic garnet film sandwiched between two metallic layers, patterned with periodically spaced parallel grooves on their outer sides. Our results are analyzed by reference to the properties of the individual structural components and a consistent interpretation of the different spectral features observed is provided. It is shown that, by properly adjusting the geometrical parameters involved, strong Faraday rotation enhancement can be obtained through constructive synergy between the Fabry–Pérot resonant mode of the magneto-optical nanocavity and the slot plasmon mode localized in the grooves.
A century-old tenet in physics and engineering asserts that any type of system, having bandwidth Dw, can interact with a wave over only a constrained time period Dt inversely proportional to the bandwidth (Dt·Dw ∼ 2p). This law severely limits the generic capabilities of all types of resonant and wave-guiding systems in photonics, cavity quantum electrodynamics and optomechanics, acoustics, continuum mechanics, and atomic and optical physics but is thought to be completely fundamental, arising from basic Fourier reciprocity.We propose that this "fundamental" limit can be overcome in systems where Lorentz reciprocity is broken. As a system becomes more asymmetric in its transport properties, the degree to which the limit can be surpassed becomes greater. By way of example, we theoretically demonstrate how, in an astutely designed magnetized semiconductor heterostructure, the above limit can be exceeded by orders of magnitude by using realistic material parameters. Our findings revise prevailing paradigms for linear, time-invariant resonant systems, challenging the doctrine that high-quality resonances must invariably be narrowband and providing the possibility of developing devices with unprecedentedly high time-bandwidth performance.
This article is divided in three sections; the first is presenting the plethora of fiber optic and power cable geographical distribution, providing to the reader an insight to underwater infrastructure. Readers can bind together the importance of survey, lay, repair and maintenance of underwater cables and pipelines, based on the legal framework provided by the Energy Treaty and UNCLOS. The second section provides a brief report on notions mentioned in the International Energy Treaty. We also mention the Energy Treaty and the Energy Treaty Charter as a case study for pipeline and power cable installations; At the third section, we focus especially at the genesis of UNCLOS with regards to the EEZ maritime zone regime; At this section, we will present the difference between the terms marine research and cable route study, based on the applicable UNCLOS conventional terminology. The last section of this article will be an effort to carefully examine whether these two Treaties are sufficient to regulate transnational cable surveying and laying operations under the current geopolitical frame in the Eastern Mediterranean Sea; as new oil and gas fields are being developed in the region two factors affect the geopolitical equilibrium: 1) The disputed EEZ's in the Eastern Mediterranean, 2) The role of Turkey based on its destabilizing and revisionist stand as this is highlighted by extreme geopolitical behaviors causing the present instability in the geopolitical environment of the Eastern Mediterranean, mainly with regards to the supply of energy from discovered gas reservoirs in the Eastern Mediterranean.
This article examines patterns of reception through which a particular type of classical sculpture – the Caryatid – has been accepted into the cultural life of contemporary Greece. Loved by neoclassical architecture, though also prominent in Modern Greek design, as well as contemporary poetry and drama, the Caryatid serves as a logo for the country and the Greeks at large, especially when referring to their relations with their fellow-Europeans. In contemporary Greek culture, Caryatids are deployed as symbols of Greekness as well as a means to achieve the nation’s cultural emancipation against the supremacy of western, globalised modernity. Often derided as mere symptoms of colonial mimicry, through their inherent qualities of parody and subversiveness, such uses may sometimes prove unexpectedly successful in undermining modernity and its templates.
The reaction of the bis(pentalene)dititanium complex Ti2(μ:η5,η5-Pn)2 (Pn = C8H4(1,4-SiiPr3)2) with the N-heterocyclic carbene 1,3,4,5-tetramethylimidazol-2-ylidene results in intramolecular C-H activation of one of the iPr methyl groups of a Pn ligand and formation of a "tucked-in" bridging hydride complex. The "tuck-in" process is reversed by the addition of hydrogen, which yields a dihydride featuring terminal and bridging hydrides.
Fractures of the odontoid process seem to have an unclear and not well-understood behavior. This is well demonstrated in the literature as there is a disagreement in many fields, including the patterns of fractures, the percentage of pseudarthrosis or the appropriate treatment that is required. This situation makes the fracture itself a "challenging" one. Indeed, despite the existence of extensive literature, there are still many unresolved problems concerning these fractures. We recognize six main classification schemes of the odontoid process fractures which clearly demonstrate the presence of differences but, also, the disadvantages that these classifications are related to. The most important factors that render these fractures "enigmatic" are the anatomy of the odontoid, the diversity of fractures encountered, the radiological evaluation, the stability and the inability to incorporate the whole spectrum of fractures in one classification. The progress made the last decades in the field of the radiology and their follow-up permitted to better analyze these lesions and to extract useful conclusions.
Jets in long-duration γ-ray bursts (GRBs) have to drill through the collapsing star in order to break out of it and produce the γ-ray signal while the central engine is still active. If the breakout time is shorter for more powerful engines, then the jet-collapsar interaction acts as a filter of less luminous jets. We show that the observed broken power-law GRB luminosity function is a natural outcome of this process. For a theoretically motivated breakout time that scales with jet luminosity as L-χ with χ ∼ 1/3-1/2, we show that the shape of the γ-ray duration distribution can be uniquely determined by the GRB luminosity function and matches the observed one. This analysis has also interesting implications about the supernova-central engine connection. We show that not only successful jets can deposit sufficient energy in the stellar envelope to power the GRB-associated supernovae, but also failed jets may operate in all Type Ib/c supernovae.
BACKGROUND: Posture, vision, and instrumentation limitations are the main predicaments of conventional laparoscopy. OBJECTIVE: To combine the ETHOS surgical chair, the three-dimensional laparoscope, and the Radius Surgical System manipulators, and compare the system with conventional laparoscopy and da Vinci in terms of task completion times and discomfort. DESIGN, SETTING, AND PARTICIPANTS: Fifteen trainees performed the three main laparoscopic suturing tasks of the Heilbronn training program (IV: simulation of dorsal venous complex suturing; V: circular suturing of tubular structure; and VI: urethrovesical anastomosis) in a pelvi trainer. The tasks were performed conventionally, utilizing the three devices, and robotically. Task completion times were recorded and the surgeon discomfort was evaluated using questionnaires. OUTCOME MEASUREMENTS AND STATISTICAL ANALYSIS: Task completion times were compared using nonparametric Wilcoxon signed rank test and ergonomic scores were compared using Pearson chi-square test. RESULTS AND LIMITATIONS: The use of the full laparoscopic set (ETHOS chair, three-dimensional laparoscopic camera, Radius Surgical System needle holders), resulted in a significant improvement of the completion time of the three tested tasks compared with conventional laparoscopy (p<0.001) and similar to da Vinci surgery. After completing Tasks IV, V, and VI conventionally, 12 (80%), 13 (86.7%), and 13 (86.7%) of the 15 trainees, respectively, reported heavy total discomfort. The full laparoscopic system nullified heavy discomfort for Tasks IV and V and minimized it (6.7%) for the most demanding Task VI. Especially for Task VI, all trainees gained benefit, by using the system, in terms of task completion times and discomfort. The limited trainee robotic experience and the questionnaire subjectivity could be a potential limitation. CONCLUSIONS: The ergonomic laparoscopic system offers significantly improved task completion times and ergonomy than conventional laparoscopy. Furthermore, it demonstrates comparable results to robotic surgery. PATIENT SUMMARY: The study was conducted in a pelvi trainer and no patients were recruited.
AIM: To investigate the effectiveness of ultrasound-guided release of the first annular pulley and compare results with the conventional open operative technique.
METHODS: In this prospective randomized, single-center, clinical study, 32 patients with trigger finger or trigger thumb, grade II-IV according to Green classification system, were recruited. Two groups were formed; Group A (16 patients) was treated with an ultrasound-guided percutaneous release of the affected A1 pulley under local anesthesia. Group B (16 patients) underwent an open surgical release of the A1 pulley, through a 10-15 mm incision. Patients were assessed pre- and postoperatively (follow-up: 2, 4 and 12 wk) by physicians blinded to the procedures. Treatment of triggering (primary variable of interest) was expressed as the "success rate" per digit. The time for taking postoperative pain killers, range of motion recovery, QuickDASH test scores (Greek version), return to normal activities (including work), complications and cosmetic results were assessed.
RESULTS: The success rate in group A was 93.75% (15/16) and in group B 100% (16/16). Mean times in group A patients were 3.5 d for taking pain killers, 4.1 d for returning to normal activities, and 7.2 and 3.9 d for complete extension and flexion recovery, respectively. Mean QuickDASH scores in group A were 45.5 preoperatively and, 7.5, 0.5 and 0 after 2, 4, and 12 wk postoperatively. Mean times in group B patients were 2.9 d for taking pain killers, 17.8 d for returning to normal activities, and 5.6 and 3 d for complete extension and flexion recovery. Mean QuickDASH scores in group B were 43.2 preoperatively and, 8.2, 1.3 and 0 after 2, 4, and 12 wk postoperatively. The cosmetic results found excellent or good in 87.5% (14/16) of group A patients, while in 56.25% (9/16) of group B patients were evaluated as fair or poor.
CONCLUSION: Treatment of the trigger finger using ultrasonography resulted in fewer absence of work days, and better cosmetic results, in comparison with the open surgery technique. It is a promising method that represents excellent results without major complications, so that it could be possibly be established as a first-line treatment in the trigger finger's disease.
BACKGROUND: The fungal genus Aspergillus is of critical importance to humankind. Species include those with industrial applications, important pathogens of humans, animals and crops, a source of potent carcinogenic contaminants of food, and an important genetic model. The genome sequences of eight aspergilli have already been explored to investigate aspects of fungal biology, raising questions about evolution and specialization within this genus.
RESULTS: We have generated genome sequences for ten novel, highly diverse Aspergillus species and compared these in detail to sister and more distant genera. Comparative studies of key aspects of fungal biology, including primary and secondary metabolism, stress response, biomass degradation, and signal transduction, revealed both conservation and diversity among the species. Observed genomic differences were validated with experimental studies. This revealed several highlights, such as the potential for sex in asexual species, organic acid production genes being a key feature of black aspergilli, alternative approaches for degrading plant biomass, and indications for the genetic basis of stress response. A genome-wide phylogenetic analysis demonstrated in detail the relationship of the newly genome sequenced species with other aspergilli.
CONCLUSIONS: Many aspects of biological differences between fungal species cannot be explained by current knowledge obtained from genome sequences. The comparative genomics and experimental study, presented here, allows for the first time a genus-wide view of the biological diversity of the aspergilli and in many, but not all, cases linked genome differences to phenotype. Insights gained could be exploited for biotechnological and medical applications of fungi.
Photometric measurements are prone to systematic errors presenting a challenge to low-amplitude variability detection. In search for a general-purpose variability detection technique able to recover a broad range of variability types including currently unknown ones, we test 18 statistical characteristics quantifying scatter and/or correlation between brightness measurements. We compare their performance in identifying variable objects in seven time series data sets obtained with telescopes ranging in size from a telephoto lens to 1 m-class and probing variability on time-scales from minutes to decades. The test data sets together include light curves of 127 539 objects, among them 1251 variable stars of various types and represent a range of observing conditions often found in ground-based variability surveys. The real data are complemented by simulations. We propose a combination of two indices that together recover a broad range of variability types from photometric data characterized by a wide variety of sampling patterns, photometric accuracies and percentages of outlier measurements. The first index is the interquartile range (IQR) of magnitude measurements, sensitive to variability irrespective of a time-scale and resistant to outliers. It can be complemented by the ratio of the light-curve variance to the mean square successive difference, 1/η, which is efficient in detecting variability on time-scales longer than the typical time interval between observations. Variable objects have larger 1/η and/or IQR values than non-variable objects of similar brightness. Another approach to variability detection is to combine many variability indices using principal component analysis. We present 124 previously unknown variable stars found in the test data.
This study compared the effects of unilateral and bilateral plyometric training on single and double-leg jumping performance, maximal strength and rate of force development (RFD). Fifteen moderately trained subjects were randomly assigned to either a unilateral (U, n=7) or bilateral group (B, n=8). Both groups performed maximal effort plyometric leg exercises two times per week for 6 weeks. The B group performed all exercises with both legs, while the U group performed half the repetitions with each leg, so that total exercise volume was the same. Jumping performance was assessed by countermovement jumps (CMJ) and drop jumps (DJ), while maximal isometric leg press strength and RFD were measured before and after training for each leg separately and both legs together. CMJ improvement with both legs was not significantly different between U (12.1±7.2%) and B (11.0±5.5%) groups. However, the sum of right and left leg CMJ only improved in the U group (19.0±7.1%, p<0.001) and was unchanged in the B group (3.4±8.4%, p=0.80). Maximal isometric leg press force with both legs was increased similarly between groups (B: 20.1±6.5%, U: 19.9±6.2%). However, the sum of right and left leg maximal force increased more in U compared to B group (23.8±9.1% vs. 11.9±6.2%, p=0.009, respectively). Similarly, the sum of right and left leg RFD0-50 and RFD0-100 were improved only in the U group (34-36%, p<0.01). Unilateral plyometric training was more effective at increasing both single and double-leg jumping performance, isometric leg press maximal force and RFD when compared to bilateral training.
Molecular mechanisms governing maintenance, commitment, and differentiation of stem cells are largely unexploited. Molecules involved in the regulation of multiple cellular processes are of particular importance for stem cell physiology, as they integrate different signals and coordinate cellular decisions related with self-renewal and fate determination. Geminin has emerged as a critical factor in DNA replication and stem cell differentiation in different stem cell populations. Its inhibitory interaction with Cdt1, a member of the prereplicative complex, ensures the controlled timing of DNA replication and, consequently, genomic stability in actively proliferating cells. In embryonic as well as somatic stem cells, Geminin has been shown to interact with transcription factors and epigenetic regulators to drive gene expression programs and ultimately guide cell fate decisions. An ever-growing number of studies suggests that these interactions of Geminin and proteins regulating transcription are conserved among metazoans. Interactions between Geminin and proteins modifying the epigenome, such as members of the repressive Polycomb group and the SWI/SNF proteins of the permissive Trithorax, have long been established. The complexity of these interactions, however, is only just beginning to unravel, revealing key roles on maintaining stem cell self-renewal and fate specification. In this review, we summarize current knowledge and give new perspectives for the role of Geminin on transcriptional and epigenetic regulation, alongside with its regulatory activity in DNA replication and their implication in the regulation of stem and progenitor cell biology. Stem Cells 2017;35:299-310.
Moschos MM, Chatziralli I, Gazouli M, Brouzas D, Cennamo G, Eidenberger T, Forte R, Panzella L, Cesarano I, Napolitano A, et al.Contents Vol. 58, 2017. Ophthalmic Research. 2017;58:I–IV.
BACKGROUND AND PURPOSE: Vasovagal syncope (VVS) is linked to more than one pathophysiologic mechanisms. Copeptin, an emerging cardiovascular marker, is a surrogate for arginine-vasopressin, which increases following VVS. We aimed to assess the dynamic pattern of copeptin levels in typical VVS, categorized by the degree of vasoconstriction during orthostasis, and healthy controls. METHODS: The following groups were studied: Group A (n=21), with adequate limb vasoconstriction during the first min. of tilt, assessed by limb plethysmography (at least 30% flow reduction); Group B (n=15), showing impaired vasoconstriction during orthostasis (<10% reduction); Group C (n=18), history of VVS and negative tilt test result; Group D (n=18), healthy controls. Copeptin plasma levels were assessed before and 5min following tilt test positivity or termination. RESULTS: Baseline copeptin values were similar in all groups (8.3+/-6.4 in Group A, 5.7+/-2.3pmol/l in B, 6.0+/-1.9 in C, and 6.9+/-2.6 in D, p: 0.41). Significant increases in copeptin during tilt were observed in all Groups of VVS patients (A, B, C), including those with negative tilt (Group C: from 6.0+/-1.9 to 27.7+/-12.6pmol/l, p: 0.001), but not in controls. Following tilt termination, a greater increase was observed in copeptin values in Group B vs all other Groups A, C, and D (111.6+/-63.5 vs 29.5+/-51.3, 27.7+/-12.6, and 8.3+/-2.9, respectively). CONCLUSIONS: Copeptin increases following tilt not only in VVS with a positive response, but also in typical history patients with a negative test. Increased copeptin levels following orthostasis may be useful for diagnosing VVS
Aim : To evaluate the shape of the craniofacial complex in patients with tooth agenesis and compare it to matched controls.
Subjects and methods : The sample comprised 456 patients that were allocated to three groups: the agenesis group of 100 patients with at least one missing tooth, excluding third molars, the third molar agenesis group (3dMAG; one to four missing third molars) of 52 patients and the control group (CG) of 304 patients with no missing teeth. The main craniofacial structures depicted on lateral cephalograms were digitized and traced with 15 curves and 127 landmarks. These landmarks were subjected to Procrustes superimposition and principal component analysis in order to describe shape variability of the cranial base, maxilla and mandible, as well as of the whole craniofacial complex. For statistical analysis, permutation tests were used (10 000 permutations without replacement).
Results : Approximately half of the sample's variability was described by the first three principal components. Comparisons within the whole sample revealed sexual dimorphism of the craniofacial complex and its structures (P < 0.01). Differences between the agenesis group and matched controls were found in the shape of all craniofacial structures except for the cranial base (P < 0.05). Specifically, patients with agenesis presented with Class III tendency and hypodivergent skeletal pattern. However, the comparison between the 3dMAG and matched CG revealed no differences.
Conclusion : The shape of the craniofacial complex differs in patients with tooth agenesis suggesting that common factors are implicated in tooth development and craniofacial morphology.
This study investigated emerging adulthood and transition to adulthood in Greece, a highly underresearched issue in this country. Participants were 784 university students aged 17.5-27.5 years. Criteria for the transition to adulthood, developmental features of emerging adulthood, perceived adult status, views of the future (optimism), and sociodemographic variables were assessed. The results support the existence of emerging adulthood as a distinct life period in Greece. More than two thirds of the sample were self-perceived emerging adults. Most prevalent criteria were Norm compliance and Family capacities. Developmental features of emerging adulthood ranked high, especially Identity exploration, Experimentation/possibilities, and Feeling “in-between”. Statistically significant variations emerged as a function of gender, age, living arrangement, job experience, and perceived adult status. Views of the future were cautiously optimistic. Similarities with existing data and differences related to the specific characteristics of the Southern European context are discussed.
Today's rich digital information environment is characterized by the multitude of data sources providing information that has not yet reached its full potential in eHealth. The aim of the presented approach, namely CrowdHEALTH, is to introduce a new paradigm of Holistic Health Records (HHRs) that include all health determinants. HHRs are transformed into HHRs clusters capturing the clinical, social and human context of population segments and as a result collective knowledge for different factors. The proposed approach also seamlessly integrates big data technologies across the complete data path, providing of Data as a Service (DaaS) to the health ecosystem stakeholders, as well as to policy makers towards a "health in all policies" approach. Cross-domain co-creation of policies is feasible through a rich toolkit, being provided on top of the DaaS, incorporating mechanisms for causal and risk analysis, and for the compilation of predictions.
NCS1 proteins are H(+) or Na(+) symporters responsible for the uptake of purines, pyrimidines or related metabolites in bacteria, fungi and some plants. Fungal NCS1 are classified into two evolutionary and structurally distinct subfamilies, known as Fur- and Fcy-like transporters. These subfamilies have expanded and functionally diversified by gene duplications. The Fur subfamily of the model fungus Aspergillus nidulans includes both major and cryptic transporters specific for uracil, 5-fluorouracil, allantoin or/and uric acid. Here we functionally analyse all four A. nidulans Fcy transporters (FcyA, FcyC, FcyD and FcyE) with previously unknown function. Our analysis shows that FcyD is moderate-affinity, low-capacity, highly specific adenine transporter, whereas FcyE contributes to 8-azaguanine uptake. Mutational analysis of FcyD, supported by homology modelling and substrate docking, shows that two variably conserved residues (Leu356 and Ser359) in transmembrane segment 8 (TMS8) are critical for transport kinetics and specificity differences among Fcy transporters, while two conserved residues (Phe167 and Ser171) in TMS3 are also important for function. Importantly, mutation S359N converts FcyD to a promiscuous nucleobase transporter capable of recognizing adenine, xanthine and several nucleobase analogues. Our results reveal the importance of specific residues in the functional evolution of NCS1 transporters.
The methylation of U1498 located in the 16S ribosomal RNA of Escherichia coli is an important modification affecting ribosomal activity. RsmE methyltransferases methylate specifically this position in a mechanism that requires an S-adenosyl-L-methionine (AdoMet) molecule as cofactor. Here we report the structure of Apo and AdoMet-bound Lpg2936 from Legionella pneumophila at 1.5 and 2.3 A, respectively. The protein comprises an N-terminal PUA domain and a C-terminal SPOUT domain. The latter is responsible for protein dimerization and cofactor binding. Comparison with similar structures suggests that Lpg2936 is an RsmE-like enzyme that can target the equivalent of U1498 in the L. pneumophila ribosomal RNA, thereby potentially enhancing ribosomal activity during infection-mediated effector production. The multiple copies of the enzyme found in both structures reveal a flexible conformation of the bound AdoMet ligand. Isothermal titration calorimetry measurements suggest an asymmetric two site binding mode. Our results therefore also provide unprecedented insights into AdoMet/RsmE interaction, furthering our understanding of the RsmE catalytic mechanism.
A family of benzotriazole based coordination compounds, obtained in two steps and good yields from commercially available materials, formulated as [CuII(L1)2(MeCN)2]·2ClO4·MeCN (1), [CuII(L1)(NO3)2]·MeCN (2), [ZnII(L1)2(H2O)2]·2ClO4·2MeCN (3), [CuII(L1)2Cl2]2 (4), [CuII5(L1)2Cl10] (5), [CuII2(L1)4Br2]·4MeCN·CuII2Br6 (6), [CuII(L1)2(MeCN)2]·2BF4 (7), [CuII(L1)2(CF3SO3)2] (8), [ZnII(L1)2(MeCN)2]·2CF3SO3 (9), [CuII2(L2)4(H2O)2]·4CF3SO3·4Me2CO (10), and [CuII2(L3)4(CF3SO3)2]·2CF3SO3·Me2CO (11), are reported. These air-stable compounds were tested as homogeneous catalysts for the A3 coupling synthesis of propargylamine derivatives from aldehyde, amine, and alkyne under a noninert atmosphere. Fine tuning of the catalyst resulted in a one-dimensional (1D) coordination polymer (CP) (8) with excellent catalytic activity in a wide range of substrates, avoiding any issues that would inhibit its performance.
Early management of vascular injury, starting at the field, is imperative for survival no less than any operative maneuver. Contemporary prehospital management of vascular trauma, including appropriate fluid and volume infusion, tourniquets, and hemostatic agents, has reversed the historically known limb hemorrhage as a leading cause of death. In this context, damage control (DC) surgery has evolved to DC resuscitation (DCR) as an overarching concept that draws together preoperative and operative interventions aiming at rapidly reducing bleeding from vascular disruption, optimizing oxygenation, and clinical outcomes. This review addresses contemporary DCR techniques from the prehospital to the surgical setting, focusing on civilian vascular injuries.
BACKGROUND: New diagnoses of HIV-1 infection among people who inject drugs (PWID) increased significantly during 2011 in Athens.
OBJECTIVE: Our aim was to investigate the patterns of HIV epidemic spread among PWID and to estimate the transmission dynamics for the major local transmission networks (LTNs).
METHODS: We analyzed sequences from 2,274 HIV-infected subjects sampled in Greece during 01/01/2011-31/10/2014. Of specimens in our sample, 874 sequences were isolated from HIV-infected PWID. Phylodynamic analysis was performed using birth-death serial skyline models.
RESULTS: Phylogenetic analysis revealed that the majority of sequences from PWID (N=746, 85.4%) fell within four LTNs: CRF14_BG (N=456, 58.3%), CRF35_AD (N=149, 19.1%), subtype B (N=118, 15.1%) and A1 (N=59, 7.5%). In addition to PWID, we also found that sequences from 36 non-PWID belonged to the LTNs corresponding to cross-group transmissions. Based on the estimated plots of the effective reproductive number (Re) over time, subtype A1 and CRF35_AD LTNs showed a sharp increase before and during 2011 (maximum value of Re=3.0 and Re=4.6, respectively). For subtype B and CRF14_BG LTNs, the Re was increasing until the end of 2012 (maximum value of Re=3.2 and Re=3.0, respectively).
CONCLUSION: HIV transmissions within subtype A1 and CRF35_AD LTNs increased sharply during the early stage of the outbreak, in contrast to subtype B and CRF14_BG. A significant reduction in the number of infections was estimated on all transmission networks from the beginning of 2013 onwards. Prevention measures that took place in the Athens metropolitan area at the end of 2012 including also the ARISTOTLE program may explain this decrease.
Swift_J010745.0-722740 is a BeXRB candidate located in the SMC (ATel #5778) with no X-ray pulsations detected so far. Its companion exhibits strong (0.3-0.4 mag) outbursts, with a recurring time of 1180 d (ATel #5781, #5778).
The paper starts recalling the various stages that lead to the definition of the Early Byzantine Tectonic Paroxysm (EBTP), which was determined mainly from fossil uplifted shorelines of the Eastern Mediterranean between the middle of the 4th century and the middle of the 6th century CE. A comparison of catalogues of earthquakes may show that possibly an important event occurred in 361 CE in the northern Adriatic, i.e. near the beginning of a sequence of clustered earth movements. This event is proposed as the most likely for the coseismic subsidence of a known slightly submerged tidal notch that developed along over 200 km of the Croatian shoreline.
The aim of this preliminary study is to present the usage behaviour of mHealth applications. A survey was conducted among 352 Smartphone users; 109 of them had used mHealth applications as well. The usage of these applications seemed to be quite low. Young people had used mHealth applications more than the elders. Also, there was not found a statistically significant relationship between the gender and the usage of mHealth applications.
. Documents pour l'histoire du français langue étrangère ou seconde [Internet]. 2017;58-59.
Le début du XXe siècle, est pour la Grèce une période de transition entre la méthode traditionnelle et la méthode directe. On se préoccupe davantage pour l’enseignement de la langue vivante et de son aspect plus pratique. Ainsi, de nombreux pédagogues, encouragés par la multiplication des instituts de langue, écoles commerciales mais aussi par l’intérêt croissant de la jeunesse hellénique pour les concours administratifs (qui exigeaient une bonne connaissance de la langue française) proposent des nouvelles méthodes d’apprentissage du français et font carrière en publiant des ouvrages divers pour l’enseignement de la langue. C’est dans ce cadre que paraît à Athènes, de 1908 à 1911, Ἡ Γαλλική Ἐφημερίς, une publication hebdomadaire bilingue. Son éditeur, Théodore Kyprios, professeur de français et auteur par la suite de nombreux manuels, se vante de mettre à la disposition des apprenants grecs un outil innovant pour l’apprentissage de la langue française. Il s’agit, en effet, d’une revue destinée à l’autoapprentissage de la langue. Tout en restant près de la tradition scolaire qui continue à promouvoir l’usage de la traduction dans l’enseignement des langues, Ἡ Γαλλική Ἐφημερίς vise à une maîtrise effective de la langue, comme moyen de communication, et à des connaissances vraiment utiles et indispensables dans les relations sociales quotidiennes. Il s’applique, enfin, à donner à ses lecteurs le contexte culturel dans lequel évolue le peuple dont on étudie la langue
PURPOSE: Despite recent research advantages on the molecular and subcellular background, bladder cancer (BlCa) remains a clinically neglected malignancy. This is strongly reflected by the generic approach of disease diagnosis and management. Additionally, patients' prognosis became a rather demanding task due to the great disease heterogeneity. Here, we aimed to evaluate, for the first time, the clinical value of KLK13 in BlCa. METHODS: A total of 279 bladder specimens (137 tumors, 107 adjacent normal tissues and 35 healthy samples) were included. Total RNA was extracted, reverse transcribed, and KLK13 expression was assessed by quantitative real-time PCR. RESULTS: KLK13 expression is significantly increased in bladder tumors compared to normal adjacent epithelium. However, reduced KLK13 expression is correlated with disease aggressiveness, including higher tumor stage and grade, and high-risk TaT1 tumors according to the EORTC stratification. Moreover, Kaplan-Meier and Cox regression analysis highlighted the prognostic value of the reduced KLK13 expression for the prediction of TaT1 patients' recurrence and shorter disease-free survival following TURBT. Finally, the combination of KLK13 expression with EORTC-risk stratification results to an improved prediction of TaT1 patients' outcome. CONCLUSION: This first clinical study of KLK13 in BlCa reveals its deregulated expression in bladder tumors and highlights KLK13 as a promising marker for improving TaT1 patients' prognosis following treatment.
Background: Necrotizing fasciitis (NF) is a group of relatively rare infections, usually caused by two or more pathogens. It affects the skin and subcutaneous tissues of lower and upper limbs, perineal area (Fournier’s gangrene) and the abdominal wall. Early diagnosis and aggressive surgical management are of high significance for the management of this potentially lethal disease. Methods: We conducted a retrospective study in patients who presented,during the last decade,at four University Surgical Departments in the area of Athens, Greece, with an admission diagnosis of NF. Demographic, clinical and laboratory data were gathered, and the preoperative and surgical treatment, as well as the postoperative treatment was analyzed for these patients. Results: A total of 62 patients were included in the study. The mean age of patients was 63.7 (47 male patients). Advanced age (over 65 years) (P<0.01) and female sex (P=0.04) correlated significantly with mortality. Perineum was the mostly infected site (46.8%), followed by the lower limbs (35.5%), and the upper limbs and the axillary region (8.1%). Diabetes mellitus was the most common co-existing disease (40.3%), followed by hypertension (25.8%) and obesity (17,7%). The most common symptom was local pain and tenderness (90.3%). Septic shock occurred in 8 patients (12.9%), and strongly correlated with mortality (P<0.01). Laboratory data were used to calculate retrospectively the LRINEC score of every patient; 26 patients (41.9%) had LRINEC score under 6, 20 patients (32.3%) had LRINEC score 6-8 and 16 patients (25.8%) had LRINEC score >9. Surgical debridement was performed in all patients (mean no. of repeated debridement 4.8), and in 16 cases (25.8%) the infected limb was amputated. The mean length of hospital stay was 19.7 days, and the overall mortality rate of our series was 17.7%. Conclusions: Diagnosis of NF requires high suspect among clinicians, as its clinical image is non-specific. Laboratory tests can depict the severity of the disease; therefore they must be carefully evaluated. Urgent surgical debridement is the mainstay of treatment in all patients; the need of repetitive surgical debridement is undisputed.
Age-dependent collapse of lipid homeostasis results in spillover of lipids and excessive fat deposition in nonadipose tissues. Ectopic fat contributes to lipotoxicity and has been implicated in the development of a metabolic syndrome that increases risk of age-associated diseases. However, the molecular mechanisms coupling ectopic fat accumulation with aging remain obscure. Here, we use nonlinear imaging modalities to visualize and quantify age-dependent ectopic lipid accumulation in Caenorhabditis elegans We find that aging is accompanied by pronounced deposition of lipids in nonadipose tissues, including the nervous system. Importantly, interventions that promote longevity such as low insulin signaling, germ-line loss, and dietary restriction, which effectively delay aging in evolutionary divergent organisms, diminish the rate of ectopic fat accumulation and the size of lipid droplets. Suppression of lipotoxic accumulation of fat in heterologous tissues is dependent on helix-loop-helix (HLH)-30/transcription factor EB (TFEB) and autophagy. Our findings in their totality highlight the pivotal role of HLH-30/TFEB and autophagic processes in the maintenance of lipid homeostasis during aging, in addition to establishing nonlinear imaging as a powerful tool for monitoring ectopic lipid droplet deposition in vivo.
The use of dopamine replacement therapies (DRT) in the treatment of Parkinson's disease (PD) can lead to the development of dopamine dysregulation syndrome (DDS) and impulse control disorders (ICD), behavioral disturbances characterized by compulsive DRT self-medication and development of impulsive behaviors. However, the mechanisms behind these disturbances are poorly understood. In animal models of PD, the assessment of the rewarding properties of levodopa (LD), one of the most common drugs used in PD, has produced conflicting results, and its ability to promote increased impulsivity is still understudied. Moreover, it is unclear whether acute and chronic LD therapy differently affects reward and impulsivity. In this study we aimed at assessing, in an animal model of PD with bilateral mesostriatal and mesocorticolimbic degeneration, the behavioral effects of LD therapy regarding reward and impulsivity. Animals with either sham or 6-hydroxydopamine (6-OHDA)-induced bilateral lesions in the substantia nigra pars compacta (SNc) and ventral tegmental area (VTA) were exposed to acute and chronic LD treatment. We used the conditioned place preference (CPP) paradigm to evaluate the rewarding effects of LD, whereas impulsive behavior was measured with the variable delay-to-signal (VDS) task. Correlation analyses between behavioral measurements of reward or impulsivity and lesion extent in SNc/VTA were performed to pinpoint possible anatomical links of LD-induced behavioral changes. We show that LD, particularly when administered chronically, caused the development of impulsive-like behaviors in 6-OHDA-lesioned animals in the VDS. However, neither acute or chronic LD administration had rewarding effects in 6-OHDA-lesioned animals in the CPP. Our results show that in a bilateral rat model of PD, LD leads to the development of impulsive behaviors, strengthening the association between DRT and DDS/ICD in PD.
The use of dopamine replacement therapies (DRT) in the treatment of Parkinson's disease (PD) can lead to the development of dopamine dysregulation syndrome (DDS) and impulse control disorders (ICD), behavioral disturbances characterized by compulsive DRT self-medication and development of impulsive behaviors. However, the mechanisms behind these disturbances are poorly understood. In animal models of PD, the assessment of the rewarding properties of levodopa (LD), one of the most common drugs used in PD, has produced conflicting results, and its ability to promote increased impulsivity is still understudied. Moreover, it is unclear whether acute and chronic LD therapy differently affects reward and impulsivity. In this study we aimed at assessing, in an animal model of PD with bilateral mesostriatal and mesocorticolimbic degeneration, the behavioral effects of LD therapy regarding reward and impulsivity. Animals with either sham or 6-hydroxydopamine (6-OHDA)-induced bilateral lesions in the (SNc) and ventral tegmental area (VTA) were exposed to acute and chronic LD treatment. We used the conditioned place preference (CPP) paradigm to evaluate the rewarding effects of LD, whereas impulsive behavior was measured with the variable delay-to-signal (VDS) task. Correlation analyses between behavioral measurements of reward or impulsivity and lesion extent in SNc/VTA were performed to pinpoint possible anatomical links of LD-induced behavioral changes. We show that LD, particularly when administered chronically, caused the development of impulsive-like behaviors in 6-OHDA-lesioned animals in the VDS. However, neither acute or chronic LD administration had rewarding effects in 6-OHDA-lesioned animals in the CPP. Our results show that in a bilateral rat model of PD, LD leads to the development of impulsive behaviors, strengthening the association between DRT and DDS/ICD in PD.
Aim of the study was to investigate whether high-intensity interval cycling performed immediately after resistance training would inhibit muscle strength increase and hypertrophy expected from resistance training per se. Twenty-two young men were assigned into either resistance training (RE; N = 11) or resistance training plus high-intensity interval cycling (REC; N = 11). Lower body muscle strength and rate of force development (RFD), quadriceps cross-sectional area (CSA) and vastus lateralis muscle architecture, muscle fiber type composition and capillarization, and estimated aerobic capacity were evaluated before and after 8 weeks of training (2 times per week). Muscle strength and quadriceps CSA were significantly and similarly increased after both interventions. Fiber CSA increased significantly and similarly after both RE (type I: 13.6 ± 3.7%, type IIA: 17.6 ± 4.4%, type IIX: 23.2 ± 5.7%, P < 0.05) and REC (type I: 10.0 ± 2.7%, type IIA: 14.8 ± 4.3% type IIX: 20.8 ± 6.0%, P < 0.05). In contrast, RFD decreased and fascicle angle increased (P < 0.05) only after REC. Capillary density and estimated aerobic capacity increased (P < 0.05) only after REC. These results suggest that high-intensity interval cycling performed after heavy-resistance exercise may not inhibit resistance exercise-induced muscle strength/hypertrophy after 2 months of training, while it prompts aerobic capacity and muscle capillarization. The addition of high-intensity cycling after heavy-resistance exercise may decrease RFD partly due to muscle architectural changes.
We investigated the effectiveness of two teaching formats that fall under the canopy of Mosston and Ashworth’s (2008) practice style, on fifth grade students’ motor skill performance and task engagement. Both formats are also known as station teaching or learning centers. In the teacher-rotated format (TR), the teacher decides the amount of time apportioned during practice at each station, whereas in the learner-rotated format (LR), each learner decides on task order and the amount of time spent at each station. Ten-year-old children (N = 60) were randomly assigned to the TR group (n = 20), the LR group (n = 25), and a control group (n = 15). A soccer dribbling test was employed to evaluate the soccer dribbling skill prior to and after the instructional intervention. The same soccer dribbling tasks were taught to the learners in both treatment groups in eight 30-min sessions. ANCOVA on the posttest scores showed a significant difference between the experimental groups and the control group (p < .001) and between the two experimental groups, favoring the LR group (p < .001). A 3 × 2 (Group × Test) repeated measures ANOVA showed a significant improvement of the soccer dribbling skill for both teaching formats (p < .001) but not for the control group. An ANOVA on the overall practice trial data yielded significant differences between the two formats, favoring the LR group (p < .001). Both formats were found to be effective, but the results indicate that given the opportunity to reapportion their practice time, learners in the LR format took advantage of this opportunity and improved their performance further.
Atomic carbon wires represent the ultimate one-atom-thick one-dimensional structure. We use a Tight-binding (TB) approach to determine the electronic structure of polyynic and cumulenic carbynes, in terms of their dispersion relations (for cyclic boundaries), eigenspectra (for fixed boundaries) and density of states (DOS). We further derive the transmission coefficient at zero-bias by attaching the carbynes to semi-infinite metallic leads, and demonstrate the effect of the coupling strength and asymmetry to the transparency of the system to incident carriers. Finally, we determine the current–voltage (I–V) characteristics of carbynes and study the effect of factors such as the weakening of the coupling of the system to one of the leads, the relative position of the Fermi levels of the carbyne and the leads, the leads' bandwidth and, finally, the difference in the energy structure between the leads. Our results confirm and reproduce some of the most recent experimental findings.
BACKGROUND AND AIM: The treatment of hepatitis C (HCV) with interferon (IFN)-free direct-acting antivirals (DAAs) is anticipated to change the future burden of disease. The aim of this study is to quantify the impact of IFN-free DAAs on HCV-related morbidity and mortality in Greece under different scenarios concerning treatment coverage and primary prevention, including the proposed by World Health Organization Global Hepatitis Strategy. METHODS: A previously described model was used to project the future disease burden up to 2030 under scenarios, which includes treatment based on the combination of pegylated-IFN with ribavirin (base case) and scenarios using DAAs therapies. RESULTS: Under the base case scenario, an increase in HCV-related morbidity and mortality is predicted in Greece (mortality in 2030: +23.6% compared with 2015). If DAAs are used with the same treatment coverage, the number of hepatocellular carcinoma cases and of liver related deaths are predicted to be lower by 4-7% compared with 2015. Under increased treatment coverage (from 2000 treated/year to approximately 5000/year in 2015-2020 and 2500/year subsequently), morbidity and mortality will decrease by 43-53% in 2030 compared with 2015. To achieve the WHO Global Hepatitis Strategy goals, a total number of 86 500 chronic hepatitis C patients will have to be treated during 2015-2030. CONCLUSIONS: Elimination of HCV in Greece by 2030 necessitates great improvements in primary prevention, implementation of large screening programs and high treatment coverage.
The authors who documented the lives of emperors –with very few exceptions, such as Procopius and Michael Psellos– were not particularly interested in their private or romantic aff airs. Occasionally they might mention imperial wives by name, but they would make extensive references to empresses only when their actions infl uenced political developments. What they neglected to do, however, was to approach those illicit incidents from a feminine point of view as well; as a result, certain questions emerge, revolving around the central reference point of the women concerned, both as parts of the regularity of marital relations and as agents leading to the disruption or circumvention. The aim of this study is to present, with the use of telling references from the above-mentioned sources, the distinct and/or interlocked “love” parts played by Byzantium’s crowned women, to attempt their categorization and to draw certain broad conclusions conerning their roles as wives, paramours and mistressess.
Social collaborative filtering recommender systems extend the traditional user-to-item interaction with explicit user-to-user relationships, thereby allowing for a wider exploration of correlations among users and items, that potentially lead to better recommendations. A number of methods have been proposed in the direction of exploring the social network, either locally (i.e. the vicinity of each user) or globally. In this paper, we propose a novel methodology for collaborative filtering social recommendation that tries to combine the merits of both the aforementioned approaches, based on the soft-clustering of the Friend-of-a-Friend (FoaF) network of each user. This task is accomplished by the non-negative factorization of the adjacency matrix of the FoaF graph, while the edge-centric logic of the factorization algorithm is ameliorated by incorporating more general structural properties of the graph, such as the number of edges and stars, through the introduction of the exponential random graph models. The preliminary results obtained reveal the potential of this idea.
This study reviews and synthesises existing information generated within the SCOPSCO (Scientific Collaboration on Past Speciation Conditions in Lake Ohrid) deep drilling project. The four main aims of the project are to infer (i) the age and origin of Lake Ohrid (Former Yugoslav Republic of Macedonia/Republic of Albania), (ii) its regional seismotectonic history, (iii) volcanic activity and climate change in the central northern Mediterranean region, and (iv) the influence of major geological events on the evolution of its endemic species. The Ohrid basin formed by transtension during the Miocene, opened during the Pliocene and Pleistocene, and the lake established de novo in the still relatively narrow valley between 1.9 and 1.3 Ma. The lake history is recorded in a 584 m long sediment sequence, which was recovered within the framework of the International Continental Scientific Drilling Program (ICDP) from the central part (DEEP site) of the lake in spring 2013. To date, 54 tephra and cryptotephra horizons have been found in the upper 460 m of this sequence. Tephrochronology and tuning biogeochemical proxy data to orbital parameters revealed that the upper 247.8 m represent the last 637 kyr. The multi-proxy data set covering these 637 kyr indicates long-term variability. Some proxies show a change from generally cooler and wetter to drier and warmer glacial and interglacial periods around 300 ka. Short-term environmental change caused, for example, by tephra deposition or the climatic impact of millennial-scale Dansgaard–Oeschger and Heinrich events are superimposed on the long-term trends. Evolutionary studies on the extant fauna indicate that Lake Ohrid was not a refugial area for regional freshwater animals. This differs from the surrounding catchment, where the mountainous setting with relatively high water availability provided a refuge for temperate and montane trees during the relatively cold and dry glacial periods. Although Lake Ohrid experienced significant environmental change over the last 637 kyr, preliminary molecular data from extant microgastropod species do not indicate significant changes in diversification rate during this period. The reasons for this constant rate remain largely unknown, but a possible lack of environmentally induced extinction events in Lake Ohrid and/or the high resilience of the ecosystems may have played a role.
Health information systems can improve the quality of services in healthcare. Evaluation of health information systems in hospitals is a very complex process, and it is more effective when different aspects are considered. The present work aims to develop an evaluation framework of an integrated HIS in order to improve the quality of healthcare services provided in Cyprus. Qualitative and quantitative methods were used, that measured three aspects of the system: technology, human factor and organization. Preliminary results showed that while participants seem to agree with the potential benefits of the integrated HIS, safety of data within the HIS is seen as a problem. Qualitatively, the study pointed to 3 factors that might improve the current integrated HIS: increasing interdisciplinary cooperation, training of staff, and upgrading the infrastructure. Further studies will provide a comprehensive picture for Cyprus, so that appropriate measures can be proposed and put in action.
ERA-Interim, ECMWF’s reanalysis product, includes wave and atmospheric characteristics, with high temporal andspatial scale, providing more information on the marine state. Even though their assimilation process has been validated and verified in numerous studies, their performance in more local scales is still under examination. This research focuses on the evaluation of performance of ERA-Interim reanalysis datasets in the Greek Seas for wind and wave characteristics in comparison to POSEIDON buoy data. The results prove fair to good correlation for wave height (r = 0.67-0.94) and wind speed (r= 0.71-0.83) and different error statistics per sub-region. The upper 10% analysis shows an underestimate of 10-15% for wind speed and wave height from ERA-Interim in relation to the buoy measurements. The ERA-Interim and the buoy monthly means and standard deviations are also presented and discussed according to seasonal patterns. The results of the study are compared to other researches of wave hindcasting and wind reanalysis data for the Greek Seas and globally. It is shown that ERA-Interim products could be regarded as representative for the Greek Seas, although their application should be made with caution regarding the assessment of extreme conditions (i.e. given in analyses of upper percentiles) and especially at nearshore locations due to complex coastline configuration enhanced by the great number of islands
{ERA-Interim, ECMWF's reanalysis product, includes wave and atmospheric characteristics, with high temporal and spatial scale, providing more information on the marine state. Even though their assimilation process has been validated and verified in numerous studies, their performance in more local scales is still under examination. This research focuses on the evaluation of performance of ERA-Interim reanalysis datasets in the Greek Seas for wind and wave characteristics in comparison to POSEIDON buoy data. The results prove fair to good correlation for wave height (r=0.67-0.94) and wind speed (r=0.71-0.83) and different error statistics per sub-region. The analysis of the upper 10% (90th percentile) shows an underestimate of 10-15% for wind speed and wave height from ERA-Interim in relation to the buoy measurements. The ERA-Interim and the buoy monthly means and standard deviations are also presented and discussed according to seasonal patterns. The results of the study are compared to other researches of wave hindcasting and wind reanalysis data for the Greek Seas and globally. It is shown that ERA-Interim products could be regarded as representative for the Greek Seas, although their application should be made with caution regarding the assessment of extreme conditions (i.e. given in analyses of upper percentiles) and especially at nearshore locations due to complex coastline configuration enhanced by the great number of islands.}
BACKGROUND: EORTC 24971 was a phase III trial demonstrating superiority of induction regimen TPF (docetaxel, cisplatin, 5-fluorouracil) over PF (cisplatin/5-fluorouracil), in terms of progression-free (PFS) and overall survival (OS) in locoregionally advanced unresectable head and neck squamous cell carcinomas. We conducted a retrospective analysis of prospectively collected data aiming to evaluate whether only HPV(-) patients (pts) benefit from adding docetaxel to PF, in which case deintensifying induction treatment in HPV(+) pts could be considered. PATIENTS AND METHODS: Pretherapy tumor biopsies (blocks or slides) were assessed for high-risk HPV by p16 immunohistochemistry, PCR and quantitative PCR. HPV-DNA+ and/or p16+ tumors were subjected to in situ hybridization (ISH) and HPV E6 oncogene expression qRT-PCR analysis. Primary and secondary objectives were to evaluate the value of HPV/p16 status as predictive factor of treatment benefit in terms of PFS and OS. The predictive effect was analyzed based on the model used in the primary analysis of the study with the addition of a treatment by marker interaction term and tested at two-sided 5% significance level. RESULTS: Of 358, 119 pts had available tumor samples and 58 of them had oropharyngeal cancer. Median follow-up was 8.7 years. Sixteen of 119 (14%) evaluable samples were p16+ and 20 of 79 (25%) evaluable tumors were HPV-DNA+. 13 of 40 pts (33%) assessed with HPV-DNA ISH and 12 of 28 pts (43%) assessed for HPV E6 mRNA were positive. The preplanned analysis showed no statistical evidence of predictive value of HPV/p16 status for PFS (P = 0.287) or OS (P = 0.118). CONCLUSIONS: The incidence of HPV positivity was low in the subset of EORTC 24971 pts analyzed. In this analysis only powered to detect a large treatment by marker interaction, there was no statistical evidence that treatment effect found overall was different in magnitude in HPV(+) or HPV(-) pts. These results do not justify selection of TPF versus PF according to HPV status.
Background: EORTC 24971 was a phase III trial demonstrating superiority of induction regimen TPF (docetaxel, cisplatin, 5-fluorouracil) over PF (cisplatin/5-fluorouracil), in terms of progression-free (PFS) and overall survival (OS) in locoregionally advanced unresectable head and neck squamous cell carcinomas. We conducted a retrospective analysis of prospectively collected data aiming to evaluate whether only HPV(-) patients (pts) benefit from adding docetaxel to PF, in which case deintensifying induction treatment in HPV(+) pts could be considered. Patients and methods: Pretherapy tumor biopsies (blocks or slides) were assessed for high-risk HPV by p16 immunohistochemistry, PCR and quantitative PCR. HPV-DNA+ and/or p16+ tumors were subjected to in situ hybridization (ISH) and HPV E6 oncogene expression qRT-PCR analysis. Primary and secondary objectives were to evaluate the value of HPV/p16 status as predictive factor of treatment benefit in terms of PFS and OS. The predictive effect was analyzed based on the model used in the primary analysis of the study with the addition of a treatment by marker interaction term and tested at two-sided 5% significance level. Results: Of 358, 119 pts had available tumor samples and 58 of them had oropharyngeal cancer. Median follow-up was 8.7 years. Sixteen of 119 (14%) evaluable samples were p16+ and 20 of 79 (25%) evaluable tumors were HPV-DNA+. 13 of 40 pts (33%) assessed with HPV-DNA ISH and 12 of 28 pts (43%) assessed for HPV E6 mRNA were positive. The preplanned analysis showed no statistical evidence of predictive value of HPV/p16 status for PFS (P = 0.287) or OS (P = 0.118). Conclusions: The incidence of HPV positivity was low in the subset of EORTC 24971 pts analyzed. In this analysis only powered to detect a large treatment by marker interaction, there was no statistical evidence that treatment effect found overall was different in magnitude in HPV(+) or HPV(-) pts. These results do not justify selection of TPF versus PF according to HPV status
This analysis assessed the utility of the limiting antigen avidity assay (LAg). Samples of people who inject drugs (PWID) in Greece with documented duration of HIV-1 infection were tested by LAg. A LAg-normalized optical density (ODn) 1.5 corresponds to a recency window period of 130 days. The proportion true recent (PTR) and proportion false recent (PFR) were estimated in 28 seroconverters and in 366 samples collected >6 months after HIV diagnosis, respectively. The association between LAg ODn and HIV RNA level was evaluated in 232 persons. The PTR was 85.7%. The PFR was 20.8% but fell to 5.9% in samples from treatment-naive individuals with long-standing infection (>1 year), and to 0 in samples with the circulating recombinant form CRF35 AD. A LAg-based algorithm with a PFR of 3.3% estimated a similar incidence trend to that calculated by analyses based on HIV-1 seroconversions. In recently infected persons indicated by LAg, the median log10 HIV RNA level was high (5.30, interquartile range 4.56-5.90). LAg can help identify highly infectious HIV(+) individuals as it accurately identifies recent infections and is correlated with the HIV RNA level. It can also produce reliable estimates of HIV-1 incidence.
This analysis assessed the utility of the limiting antigen avidity assay (LAg). Samples of people who inject drugs (PWID) in Greece with documented duration of HIV-1 infection were tested by LAg. A LAg-normalized optical density (ODn) ⩽1·5 corresponds to a recency window period of 130 days. The proportion true recent (PTR) and proportion false recent (PFR) were estimated in 28 seroconverters and in 366 samples collected >6 months after HIV diagnosis, respectively. The association between LAg ODn and HIV RNA level was evaluated in 232 persons. The PTR was 85·7%. The PFR was 20·8% but fell to 5·9% in samples from treatment-naive individuals with long-standing infection (>1 year), and to 0 in samples with the circulating recombinant form CRF35 AD. A LAg-based algorithm with a PFR of 3·3% estimated a similar incidence trend to that calculated by analyses based on HIV-1 seroconversions. In recently infected persons indicated by LAg, the median log10 HIV RNA level was high (5·30, interquartile range 4·56-5·90). LAg can help identify highly infectious HIV(+) individuals as it accurately identifies recent infections and is correlated with the HIV RNA level. It can also produce reliable estimates of HIV-1 incidence.
Context. The Exploring the X-ray Transient and variable Sky (EXTraS) project searches for coherent signals in the X-ray archival data of XMM-Newton. Aims: XMM-Newton performed more than 400 pointed observations in the region of the Large Magellanic Cloud (LMC). We inspected the results of the EXTraS period search to systematically look for new X-ray pulsators in our neighbour galaxy. Methods: We analysed the XMM-Newton observations of two sources from the 3XMM catalogue which show significant signals for coherent pulsations. Results: 3XMM J051259.8-682640 was detected as a source with a hard X-ray spectrum in two XMM-Newton observations, revealing a periodic modulation of the X-ray flux with 956 s. As optical counterpart we identify an early-type star with Hα emission. The OGLE I-band light curve exhibits a regular pattern with three brightness dips which mark a period of 1350 d. The X-ray spectrum of 3XMM J051034.6-670353 is dominated by a super-soft blackbody-like emission component (kT 70 eV) which is modulated by nearly 100% with a period of 1418 s. From GROND observations we suggest a star with r' = 20.9 mag as a possible counterpart of the X-ray source. Conclusions: 3XMM J051259.8-682640 is confirmed as a new Be/X-ray binary pulsar in the LMC. We discuss the long-term optical period as the likely orbital period which would be the longest known from a high-mass X-ray binary. The spectral and temporal properties of the super-soft source 3XMM J051034.6-670353 are very similar to those of RX J0806.3+1527 and RX J1914.4+2456 suggesting that it belongs to the class of double-degenerate polars and is located in our Galaxy rather than in the LMC.
The General Health Questionnaire – 28 (GHQ-28) is one of the most widely used screening instruments for psychiatric disorders. The aim of the present study was to examine the factor structure and the internal consistency of the Greek GHQ-28 in a sample of 501 university students (302 females; Mage = 20.5 years). Confirmatory factor analysis (CFA) did not provide an optimal fit for the original four-factor structure. Therefore, exploratory factor analysis (EFA) and principal component analysis (PCA) were conducted in search of alternative models with optimal fit. Models obtained from EFA and PCA were compared through further CFA. The model derived from EFA was the best-fitting solution and yielded three factors: Anxiety/Somatic Complaints (a unified factor), Social Dysfunction, and Severe Depression. In addition, internal consistency assessed through Cronbach's alpha was acceptable for all subscales both in the four- and in the new three-factor model. Possible explanations for the differentiations found in the structure of the Greek GHQ-28 are discussed in the framework of the existing literature on the use of this measure worldwide.
Hypnerotomachia Poliphili” (HP) is a literary source of semi-natural and human-induced landscapes that centred on seductive environmental attributes, such as gardens, groves, pergolas and dispersed ancient ruins, which represent knowledge derived from the tradition and experience of ages. HP is the short title of one of the most influential books of the Renaissance (the first edition was published by Aldus Manutius in Venice, in 1499); it is the story of a man (Poliphilos), who falls asleep and dreams of traversing landscapes and gardens containing plants, ancient ruins and allegorical figures, in order to be reunited with his beloved (Polia). Interest in this novel has been revived, due to substantial influence on landscape and garden design. In this study, attention has been given to the botanical section preserved in the text. Paying attention to narratives provides a source for (re)considering conceptions of landscapes, nature and culture, wild and domesticated plant life. Arboreal and bush plants here quoted disclose aspects of plant diversity and ecophysiology; among a large number of plant taxa, roses, myrtle, pines, cypresses, laurels, acanthus, oaks, olive trees, juniper, ivy, thyme, oregano, violets, wormwood and cyclamens were the most frequently cited. Also, the seasonality of Mediterranean landscapes, indicated by flowering and fruit-bearing indigenous plants, can be traced through textual passages. Its wood-engraved illustrations of portrayed landscapes and botanic sketches (e.g. ferns, palms, oaks, cypresses, box trees and foliage of laurel, acanthus, grapevine and ivy) constitute evidence for both plant diversity and natural history, at the end of the fifteenth-century.
The purpose of the study was to investigate the relationship between muscle fiber composition and the rate of force development (RFD) in well-trained individuals with different training background. Thirty-eight young males with different training background participated: 9 endurance runners, 10 power-trained, 9 strength-trained, and 10 sedentary. They performed maximal isometric leg press for the measurement of RFD. Body composition (dual x-ray absorptiometry) and vastus lateralis fiber type composition was also evaluated. When all participants were examined as a group, moderate correlations were found between the percent of type II muscle fibers and RFD between 100-600ms (r: 0.321 - 0.497, p<0.05). The correlation coefficients were higher for the cross-sectional area (CSA) and the %CSA of type II and IIx muscle fibers (r: 0.599 - 0.847, p<0.001). For the power group, RFD up to 250ms was highly correlated with % type IIx muscle fibers and type IIx fiber CSA (r: 0.670 - 0.826, p<0.05), as well as with %CSA of type IIx fibers (r: 0.714 - 0.975, p<0.05). Significant correlations were found between the relative RFD (·kg lower extremities lean mass) and CSA-%CSA of type II and IIx fibers for the power group (r: 0.676 - 0.903, p<0.05). No significant correlations were found between muscle morphology and RFD for the other groups. In conclusion, the present data suggest that there is a strong link between the type IIx muscle fibers and early RFD and relative RFD in power-trained participants. Type II fibers seem to be moderately linked with RFD in non-power-trained individuals.
Virtually omitted from established narratives of American modernism, yet central in the histories of the reception of European Surrealism in the US, Charles Henri Ford’s life and work have been recovered in important queer genealogies within Anglo-American modernism. Yet within this process or recovery, Ford’s poetic work is still largely overlooked, and this may have to do less with its marked Surrealist influences and/or derivative aspects than with the somewhat unclassifiable and composite texture of Ford’s poems. This article revisits Ford’s early poetry as a space of convergence and dialogue between distinct yet interrelated poetics: from the 1938 A Garden of Disorder to the 1949, Sleep in a Nest of Flames, a queer subjectivity assimilates concurrently Surrealist poetics and Djuna Barnes’s equally unclassifiable queer modernism with and against American poetic modernisms.
This article examines the nominal inflectional system of a group of Asia Minor Greek dialects (Dawkins 1910, 1916), which developed, in parallel with the fusional inflectional system, an agglutinative one due to language contact with Turkish. We argue that the ‘old’ fusional ending or the theme vowel was reanalyzed as part of the nominal stem. This novel structure was actualized by means of two competing options: in some dialects, the reanalysis was actualized transparently in all inflectional forms rendering an agglutinative pattern of inflection, whereas in dialects with limited agglutination the actualization took the form of a special type of vowel assimilation. More specifically, as part of the nominal stem, the ‘old’ theme vowel signals its merge with the root by allowing it to absorb some or all of its features. Formally, the phonological process is treated as an instance of indirect licensing (Walker 2011), according to which the theme vowel acts as a trigger due to its privileged position as a segment of the categorizer n, i.e. the head of the stem.
